An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center

Terasaka, Naohiro; Hayashi, Gosuke; Katoh, Takayuki; Suga, Hiroaki

doi:10.1038/nchembio.1549

Cited by 65 publications

(77 citation statements)

References 25 publications

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“…An attractive, general method to form aminoacyl-tRNA was developed by Suga and collaborators [9] ; a catalytic RNA (Flexizyme) recognizes and aminoacylates tRNA using an amino acid cyanomethyl ester with an aromatic side-chain as the amino acid donor. Further improvement led to dinitro-flexizyme (dFx), which utilizes amino acid 3,5-dinitrobenzyl esters (DBE) as the aminoacyl-donor without dependence on the amino acid side-chain [9a] . Flexizyme was used to produce N-terminal peptides of histone H3 containing methyl-lysine and acetyl-lysine residues; however, to date this has not been reported for full-length histone proteins [9b] .…”

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confidence: 99%

“…Further improvement led to dinitro-flexizyme (dFx), which utilizes amino acid 3,5-dinitrobenzyl esters (DBE) as the aminoacyl-donor without dependence on the amino acid side-chain [9a] . Flexizyme was used to produce N-terminal peptides of histone H3 containing methyl-lysine and acetyl-lysine residues; however, to date this has not been reported for full-length histone proteins [9b] .…”

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Dual Genetic Encoding of Acetyl‐lysine and Non‐deacetylatable Thioacetyl‐lysine Mediated by Flexizyme

et al. 2016

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Acetylation of lysine residues is an important post-translational protein modification. Lysine acetylation in histones and its crosstalk with other post-translational modifications in histone and non-histone proteins are crucial to DNA replication, DNA repair, and transcriptional regulation. We incorporated acetyl-lysine (AcK) and the non-hydrolyzable thioacetyl-lysine (ThioAcK) into full-length proteins in vitro, mediated by flexizyme. ThioAcK and AcK were site-specifically incorporated at different lysine positions into human histone H3 either individually or in pairs. We demonstrate that the thioacetyl group in histone H3 could not be removed by the histone deacetylase sirtuin type 1. This method provides a powerful tool to study protein acetylation and its role in crosstalk between posttranslational modifications.

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Dual Genetic Encoding of Acetyl‐lysine and Non‐deacetylatable Thioacetyl‐lysine Mediated by Flexizyme

et al. 2016

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“…Furthermore, the production of ribosomes in vitro may accommodate extensive modifications that could otherwise compromise fitness in vivo [50]. Recent reports of orthogonal 16S rRNA [51], orthogonal tRNAs [52], tethering 16S to 23S rRNA [53], provide the infrastructure to evolve ribosomes with radically modified functions.…”

Section: Engineering Expanded Genetic Codesmentioning

confidence: 99%

“…Additionally, orthogonal ribosomes have been engineered to exhibit useful properties that would also be deleterious for translating the rest of the proteome. An orthogonal 16S rRNA with a modified anti-Shine-Dalgarno sequence has been evolved to promote better UAG [51] and AGGA [15] suppression, an orthogonal 23S rRNA has been modified to accept orthogonal tRNAs with modified 3’ ends [52], and a 16S-23S tethered rRNA has been developed to facilitate engineering of peptidyl transferase activity [53]. Finally, release factors have been engineered to recognize additional codons [98], and EF-Tu has been modified to accept highly charged amino acids [87].…”

Section: Figurementioning

confidence: 99%

Overcoming Challenges in Engineering the Genetic Code

Lajoie

Söll

Church

2016

Journal of Molecular Biology

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Withstanding 3.5 billion years of genetic drift, the canonical genetic code remains such a fundamental foundation for the complexity of life that it is highly conserved across all three phylogenetic domains. Genome engineering technologies are now making it possible to rationally change the genetic code, offering resistance to viruses, genetic isolation from horizontal gene transfer, and prevention of environmental escape by genetically modified organisms. We discuss the biochemical, genetic, and technological challenges that must be overcome in order to engineer the genetic code.

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“…Другой путь состоит в поддержании активности eNOS и за-висимом от эндотелия расширении сосудов, опосредованными ABCG1 в ЭК, и включает извлечение ХС зрелыми α-ЛПВП [63]. Оба ЛПВП2 и ЛПВП3 стимулируют секрецию ПГI 2 ЭК [64,65].…”

Section: терапевтический архив 9 2016unclassified

The biological activity of high-density lipoprotein fractions and their role in the development of cardiovascular diseases

Babintseva

Camont

Chapman

et al. 2016

Terapevticheskii arkhiv

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1 ФГБНУ «НИИ общей патологии и патофизиологии», Москва, россия; 2 Национальный институт здоровья и медицинских исследова-ний, подразделение 1166, Госпиталь Питье-Сальпетриер, Университет Пьера и Марии Кюри, Париж, Франция; 3 ФГБоУ ВПо «рос-сийский экономический университет им. Г.В. Плеханова» Минобрнауки россии, Москва, россия Аннотация Увеличение концентрации липопротеидов высокой плотности (лПВП) в плазме крови человека может являться частью стратегии борьбы с сердечно-сосудистыми заболеваниями (ССз). частицы лПВП неоднородны по структуре, метаболизму и биологической активности. В обзоре описаны основные фракции (субпопуляции) лПВП и обсуждаются новые данные об антиатерогенных свойствах частиц лПВП. Весь спектр фракций лПВП, маленьких плотных богатых белком липопро-теидов, обладает атеропротективными свойствами, которые определяются наличием специализированных групп белков и липидов, однако такая активность может быть понижена в условиях атерогенного поражения. Всесторонний структурный и композиционный анализ лПВП позволяет обеспечить ключевую информацию для выявления фракций, обладающих характерными биологическими свойствами и теряющих функциональность при ССз. Эти фракции также могут являться биомаркерами риска развития ССз и как следствие фармакологическими мишенями. Increasing the human plasma concentration of high-density lipoproteins (hDL) may be part of strategy for control of cardiovascular diseases (cVD). hDL particles vary in their structure, metabolism, and biological activity. The review describes major hDL fractions (subpopulations) and discusses new findings on the antiatherogenic properties of hDL particles. The whole spectrum of hDL fractions, small, dense, protein-rich lipoproteins, has atheroprotective properties that are determined by the presence of specialized groups of proteins and lipids; however, this activity may be decreased in atherogenic lesion. comprehensive structural and compositional analysis of hDL may provide key information to identify the fractions that have characteristic biological properties and lose their functionality in cVD. These fractions may be also biomarkers for the risk of cVD and hence represent pharmacological targets. Keywords: cardiovascular diseases, atherosclerosis, reverse cholesterol transport, high-density lipoprotein fractions.апо -аполипопротеид апоА1 -аполипопротеид A1 ИБС -ишемическая болезнь сердца ЛПВП -липопротеиды высокой плотности ЛПНП -липопротеиды низкой плотности ЛПС -липополисахариды ЛХАТ -лецитинхолестеринацилтрансфераза ОТХС -обратный транспорт холестерина рЛПВП -реконструированные частицы ЛПВП Липопротеиды высокой плотности (ЛПВП) играют важную роль в обратном транспорте холестерина -ХС (ОТХС) -процес-се, при котором осуществляется перенос ХС от периферических тканей, в том числе артериальных стенок, в печень для дальней-шей элиминации с желчью. Данный механизм считается основ-ным, обеспечивающим опосредованную ЛПВП защиту [1,2]. Бо-лее того, частицы ЛПВП обладают целым рядом других биологи-чески активных свойств, в том числе антиоксидант...

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An orthogonal ribosome-tRNA pair via engineering of the peptidyl transferase center

Cited by 65 publications

References 25 publications

Dual Genetic Encoding of Acetyl‐lysine and Non‐deacetylatable Thioacetyl‐lysine Mediated by Flexizyme

Dual Genetic Encoding of Acetyl‐lysine and Non‐deacetylatable Thioacetyl‐lysine Mediated by Flexizyme

Overcoming Challenges in Engineering the Genetic Code

The biological activity of high-density lipoprotein fractions and their role in the development of cardiovascular diseases

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