An organotypic model of oral mucosa cells for the biological assessment of 3D printed resins for interim restorations

Álamo, Larissa; Cassiano, Fernanda Balestrero; Stuani, Vitor de Toledo; Pacheco, Leandro Edgar; Gallinari, Marjorie de Oliveira; Costa, Ciniro; Mondelli, Rafael Francisco Lia; Soares, Diana Gabriela

doi:10.1016/j.prosdent.2022.04.017

Cited by 3 publications

(2 citation statements)

References 41 publications

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“…In addition, they must have sufficient elasticity to retain a 3D structure with highly interconnected pores and appropriate mechanical characteristics. The final stability is also influenced by the gelation time, which typically depends on the crosslinking strategy [ 97 ]. Hydrogel crosslinking is often controlled by click chemistry, which minimizes side reactions and toxicity [ 98 ].…”

Section: Crosslinking Of Hamentioning

confidence: 99%

3D printed scaffolds based on hyaluronic acid bioinks for tissue engineering: a review

Chen,

Xue,

Zeng

et al. 2023

Biomater Res

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Hyaluronic acid (HA) is widely distributed in human connective tissue, and its unique biological and physicochemical properties and ability to facilitate biological structure repair make it a promising candidate for three-dimensional (3D) bioprinting in the field of tissue regeneration and biomedical engineering. Moreover, HA is an ideal raw material for bioinks in tissue engineering because of its histocompatibility, non-immunogenicity, biodegradability, anti-inflammatory properties, anti-angiogenic properties, and modifiability. Tissue engineering is a multidisciplinary field focusing on in vitro reconstructions of mammalian tissues, such as cartilage tissue engineering, neural tissue engineering, skin tissue engineering, and other areas that require further clinical applications. In this review, we first describe the modification methods, cross-linking methods, and bioprinting strategies for HA and its derivatives as bioinks and then critically discuss the strengths, shortcomings, and feasibility of each method. Subsequently, we reviewed the practical clinical applications and outcomes of HA bioink in 3D bioprinting. Finally, we describe the challenges and opportunities in the development of HA bioink to provide further research references and insights. Graphical Abstract

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Section: Crosslinking Of Hamentioning

confidence: 99%

3D printed scaffolds based on hyaluronic acid bioinks for tissue engineering: a review

Chen,

Xue,

Zeng

et al. 2023

Biomater Res

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“…Tissue-engineered 3D models of a human oral mucosa have also been used in recent years for the biological assessment of dental materials [12], to evaluate gingival softtissue attachment to different implant and abutment surfaces made of ceramic, metal, and plastic [13,14], and for studying the biological interactions between a human oral mucosa and electronic cigarettes [15].…”

Section: Introductionmentioning

confidence: 99%

Biological Evaluation of Oral Care Products Using 3D Tissue-Engineered In Vitro Models of Plaque-Induced Gingivitis

Barker,

AlQobaly,

Shaikh

et al. 2024

Dentistry Journal

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Background: The aim of this study was to investigate and visualize the anti-inflammatory and anti-bacterial effects of different oral care products using an infected and inflamed 3D tissue-engineered gingival mucosal model. Methods: A 3D full-thickness oral mucosal model was engineered inside tissue culture inserts using collagen hydrogels populated with human gingival fibroblasts and THP-1 monocytes and layered with oral epithelial cell lines. Oral saliva bacteria were cultured and added to the surface of the models and inflammation was further simulated with lipopolysaccharide (LPS) of Escherichia coli. The 3D models were exposed to three different types of toothpastes, a chlorhexidine antiseptic mouthwash, different antibiotics, and a mechanical rinse with phosphate-buffered saline (PBS) prior to biological evaluation using the PrestoBlue tissue viability assay, histology, optical coherence tomography (OCT), confocal microscopy, and measurement of the release of the inflammatory markers IL-1β, IL-6, and IL-8 with ELISA. Results: Multiple-endpoint analyses of the infected oral mucosal models treated with different anti-bacterial agents showed consistent outcomes in terms of tissue viability, histology, OCT, and confocal microscopy findings. In terms of anti-inflammatory testings, the positive control group showed the highest level of inflammation compared with all other groups. Depending on the anti-bacterial and anti-inflammatory potential of the test groups, different levels of inflammation were observed in the test groups. Conclusions: The inflamed 3D oral mucosal model developed in this study has the potential to be used as a suitable in vitro model for testing the biocompatibility, anti-inflammatory, and anti-bacterial properties of oral care products including mouthwashes and toothpastes. The results of this study indicate that the chlorhexidine mouthwash has both anti-bacterial and cytotoxic effects on the 3D oral mucosal model. Hyaluronic-acid-containing toothpaste has significant anti-bacterial and anti-inflammatory effects on the 3D oral mucosal model.

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Safety of 3D-Printed Acrylic Resins for Prosthodontic Appliances: A Comprehensive Cytotoxicity Review

Arossi,

Abdou,

Hung

et al. 2024

Applied Sciences

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Additive manufacturing resins used in dental prosthetics may retain uncured monomers post-polymerization, posing potential long-term patient exposure risks. Understanding the biological safety of these materials is crucial, particularly for 3D-printed acrylic-based prosthodontic devices such as occlusal nightguards, complete and partial dentures, and temporary fixed prostheses. This paper reviews the literature evaluating the cytotoxicity of such materials. Following the Preferred Reporting Items for Systematic Reviews and Meta-Analysis (PRISMA) guidelines, we conducted a scoping review using the MESH keywords related to population (P), intervention (I), comparison (C), and outcome (O) across databases, including OVID Medline, EMBASE, and SCOPUS. Our search, limited to peer-reviewed English language articles from 2015 to 2023, resulted in 22 papers. These studies, utilizing digital light processing (DLP) or stereolithography (SLA) printing methods, varied in examining different 3D-printed materials, as well as washing and post-curing protocols. The primary experimental cells used were human gingival fibroblasts (HGF) and mouse fibroblasts (L929). There are no statistical differences in biocompatibility regarding different commercially available resins, washing solutions, or methods. Improvements in cell viability were related to an increase in washing time, as well as post-curing time. After the polishing procedure, 3D resin-based printed occlusal devices perform similarly to milled and conventionally processed ones. Our findings underline the importance of appropriate washing and post-curing protocols in minimizing the cytotoxic risks associated with these 3D-printed resin-based devices.

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An organotypic model of oral mucosa cells for the biological assessment of 3D printed resins for interim restorations

Cited by 3 publications

References 41 publications

3D printed scaffolds based on hyaluronic acid bioinks for tissue engineering: a review

3D printed scaffolds based on hyaluronic acid bioinks for tissue engineering: a review

Biological Evaluation of Oral Care Products Using 3D Tissue-Engineered In Vitro Models of Plaque-Induced Gingivitis

Safety of 3D-Printed Acrylic Resins for Prosthodontic Appliances: A Comprehensive Cytotoxicity Review

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