An oral dsRNA delivery system based on chitosan induces G protein-coupled receptor kinase 2 gene silencing for Apolygus lucorum control

Qiao, Heng; Zhao, Jing; Xiao, Liubin; Zhu‐Salzman, Keyan; Lei, Jiaxin; Xu, Donghui; Xu, Guangchun; Tan, Yongan; Hao, Dejun

doi:10.1016/j.pestbp.2023.105481

Cited by 3 publications

(1 citation statement)

References 48 publications

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“…This feature allows it use as a nanocarrier for nucleic acid delivery. In addition to studies on mammalian cells [135][136][137], there is evidence of the successful use of chitosan/dsRNA complexes to induce the silencing of different genes in insects [138,139], including plant-feeding pests [140][141][142]. Chitosan-based nanoparticles protect dsRNA (or DNA) from degradation and unfavorable environments.…”

Section: Chitosan-based Nanocarriersmentioning

confidence: 99%

Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells

Komarova,

Ilina,

Taliansky

et al. 2023

IJMS

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Nanocarriers are widely used for efficient delivery of different cargo into mammalian cells; however, delivery into plant cells remains a challenging issue due to physical and mechanical barriers such as the cuticle and cell wall. Here, we discuss recent progress on biodegradable and biosafe nanomaterials that were demonstrated to be applicable to the delivery of nucleic acids into plant cells. This review covers studies the object of which is the plant cell and the cargo for the nanocarrier is either DNA or RNA. The following nanoplatforms that could be potentially used for nucleic acid foliar delivery via spraying are discussed: mesoporous silica nanoparticles, layered double hydroxides (nanoclay), carbon-based materials (carbon dots and single-walled nanotubes), chitosan and, finally, cell-penetrating peptides (CPPs). Hybrid nanomaterials, for example, chitosan- or CPP-functionalized carbon nanotubes, are taken into account. The selected nanocarriers are analyzed according to the following aspects: biosafety, adjustability for the particular cargo and task (e.g., organelle targeting), penetration efficiency and ability to protect nucleic acid from environmental and cellular factors (pH, UV, nucleases, etc.) and to mediate the gradual and timely release of cargo. In addition, we discuss the method of application, experimental system and approaches that are used to assess the efficiency of the tested formulation in the overviewed studies. This review presents recent progress in developing the most promising nanoparticle-based materials that are applicable to both laboratory experiments and field applications.

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Section: Chitosan-based Nanocarriersmentioning

confidence: 99%

Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells

Komarova,

Ilina,

Taliansky

et al. 2023

IJMS

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RNA interference (RNAi) mediated technique for combating plant diseases: Harnessing nanoparticles for effective delivery and enhanced efficacy

Verma,

Modgil

2024

Plant Cell Tiss Organ Cult

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Exploring chitin: novel pathways and structures as promising targets for biopesticides

Kaur,

Nagpal,

Dhingra

et al. 2024

Zeitschrift Für Naturforschung C

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Chitin, the most prevalent polymer in nature, a significant structural polysaccharide that comes in second only to cellulose. Chitin is a crucial component of fungal cell walls and also present in many other creatures, such as viruses, plants, animals, insect exoskeletons, and crustacean shells. Chitin presents itself as a promising target for the development of biopesticides. It focuses on unraveling the unique structures and biochemical pathways associated with chitin, aiming to identify vulnerabilities that can be strategically leveraged for effective and environmentally sustainable pest control. It involves a comprehensive analysis of chitinase enzymes, chitin biosynthesis, and chitin-related processes across diverse organisms. By elucidating the molecular intricacies involved in chitin metabolism, this review seeks to unveil potential points of intervention that can disrupt essential biological processes in target pests without harming non-target species. This holistic approach to understanding chitin-related pathways aims to inform the design and optimization of biopesticides with enhanced specificity and reduced ecological impact. The outcomes of this study hold great promise for advancing innovative and eco-friendly pest management strategies. By targeting chitin structures and pathways, biopesticides developed based on these findings may offer a sustainable and selective alternative to conventional chemical pesticides, contributing to the ongoing efforts towards more environmentally conscious and effective pest control solutions.

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An oral dsRNA delivery system based on chitosan induces G protein-coupled receptor kinase 2 gene silencing for Apolygus lucorum control

Cited by 3 publications

References 48 publications

Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells

Nanoplatforms for the Delivery of Nucleic Acids into Plant Cells

RNA interference (RNAi) mediated technique for combating plant diseases: Harnessing nanoparticles for effective delivery and enhanced efficacy

Exploring chitin: novel pathways and structures as promising targets for biopesticides

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