An oral chitosan DNA vaccine against nodavirus improves transcription of cell-mediated cytotoxicity and interferon genes in the European sea bass juveniles gut and survival upon infection

Valero, Yulema; Awad, Elham; Buonocore, Francesco; Arizcún, Marta; Esteban, M. Ángeles; Meseguer, José; Chaves-Pozo, Elena

doi:10.1016/j.dci.2016.06.021

Cited by 61 publications

(39 citation statements)

References 47 publications

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“…The IgT increase was more evident in gills, an important mucosal tissue, and less in spleen. The involvement of sea bass IgT in the immune response against bacterial [49] and virus [50] pathogens has been previous evidenced in gut, but this is the first time of a similar investigation in gills and spleen. It has to be noted that in rainbow trout a robust clonal response involving few variable heavy chain domain repertories has been evidenced in spleen after challenge with a rhabdovirus [29] and that it has been demonstrated that in rainbow trout, after pathogen exposure, IgT + B cells proliferate and generate specific IgT in the gills and in the skin [27, 51].…”

Section: Discussionmentioning

confidence: 85%

Immunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection

et al. 2017

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BackgroundImmunoglobulins (Igs) are fundamental components of the adaptive immune system of vertebrates, with the IgT/IgZ isotype specific of Teleosts. In this paper we describe the identification of an IgT heavy chain from the European sea bass (Dicentrarchus labrax L.), its molecular characterization and tissue mRNA localization by in situ hybridization.ResultsSea bass IgT consists of 552 aa (Accession Number KM410929) and it contains a putative 19 amino acids long signal peptide and one potential N-glycosylation site. The C-region consists of four CH domains; each contains the cysteine and tryptophan residues required for their correct folding. Based on the recent sequencing of sea bass genome, we have identified five different genomic contigs bearing exons unequivocally pertaining to IgT (CH2, CH3 and CH4), but none corresponded to a complete IgH locus as IgT sequences were found in the highly fragmented assembled genomic regions which could not be assigned to any major scaffold. The 3D structure of sea bass IgT has been modelled using the crystal structure of a mouse Ig gamma as a template, thus showing that the amino acid sequence is suitable for the expected topology referred to an immunoglobulin-like architecture. The basal expression of sea bass IgT and IgM in different organs has been analysed: gut and gills, important mucosal organs, showed high IgT transcripts levels and this was the first indication of the possible involvement of sea bass IgT in mucosal immune responses. Moreover, sea bass IgT expression increased in gills and spleen after infection with nodavirus, highlighting the importance of IgT in sea bass immune responses. In situ hybridization confirmed the presence of IgT transcripts in the gut and it revealed a differential expression along the intestinal tract, with a major expression in the posterior intestine, suggesting the hindgut as a site for the recruitment of IgT+ cells in this species. IgT transcripts were also found in gill filaments and parallel lamellae and, for the first time, we identified scattered IgT positive cells in the liver, with a strong signal in the hepatic parenchyma.ConclusionsIn conclusion, we performed a full molecular characterization of IgT in sea bass that points out its possible involvement in mucosal immune responses of this species.

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Section: Discussionmentioning

confidence: 85%

Immunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection

et al. 2017

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“…No protection was observed in turbot [346]. Whereas specific anti-NNV antibodies were only detected in grouper and Asian sea bass [347,349], the expression of cell-mediated cytotoxicity-related genes was observed in grouper and European sea bass [348,349], but it was not tested in Asian seabass [347].…”

Section: Preventionmentioning

confidence: 94%

“…Finally, the potential use of DNA vaccines to prevent VER outbreaks has been explored in turbot, grouper, European and Asian seabass [346][347][348][349][350]. The protection obtained was moderate in grouper and European sea bass (RPS: 43%-47% and 45%, respectively) [348,349], but considerably higher in Asian sea bass (RPS 77%) [350]. No protection was observed in turbot [346].…”

Section: Preventionmentioning

confidence: 99%

“…injection [289,328,329,331,332,349]. Injection has also been used for DNA vaccines [347,349] and subunit vaccines [335,336,338,[340][341][342] and oral immunization strategies have been used either for inactivated, subunit or DNA vaccines [225,[333][334][335]341,343,348,351,352]. The oral vaccine was given to the fish mixed with food [343] or by oral gavage [333,341] and encapsulated using either chemical compounds [225,348] or Artemia [353].…”

Section: Preventionmentioning

confidence: 99%

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Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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“…Chitosan has also been reported to possess natural adjuvant effects, capable of inducing DC maturation through the stimulation of type I interferon (IFN) release which has the added benefit of inducing antigen-specific Th1 responses [32]. Chitosan-based NP delivery technology has been developed over the years and has resulted in numerous developments including a potential therapeutic anti-tumour human papillomavirus (HPV) DNA vaccine [33], treatments for influenza A [34,35], viral myocarditis [36], and a host of animal-borne diseases including Newcastle disease virus (NDV) [37][38][39][40], Nodavirus [41], and Trueperella pyogenes [42]. As mentioned previously, one method to improve the efficacy of DNA vaccines is through targeted delivery to APCs which can be achieved through the functionalization of NPs.…”

Section: Polymer Nanoparticlesmentioning

confidence: 99%

Engineered Nanodelivery Systems to Improve DNA Vaccine Technologies

et al. 2020

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DNA vaccines offer a flexible and versatile platform to treat innumerable diseases due to the ease of manipulating vaccine targets simply by altering the gene sequences encoded in the plasmid DNA delivered. The DNA vaccines elicit potent humoral and cell-mediated responses and provide a promising method for treating rapidly mutating and evasive diseases such as cancer and human immunodeficiency viruses. Although this vaccine technology has been available for decades, there is no DNA vaccine that has been used in bed-side application to date. The main challenge that hinders the progress of DNA vaccines and limits their clinical application is the delivery hurdles to targeted immune cells, which obstructs the stimulation of robust antigen-specific immune responses in humans. In this updated review, we discuss various nanodelivery systems that improve DNA vaccine technologies to enhance the immunological response against target diseases. We also provide possible perspectives on how we can bring this exciting vaccine technology to bedside applications.

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An oral chitosan DNA vaccine against nodavirus improves transcription of cell-mediated cytotoxicity and interferon genes in the European sea bass juveniles gut and survival upon infection

Cited by 61 publications

References 47 publications

Immunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection

Immunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection

Betanodavirus and VER Disease: A 30-year Research Review

Engineered Nanodelivery Systems to Improve DNA Vaccine Technologies

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