An optimized protocol for the generation of HBV viral antigen-specific T lymphocytes from pluripotent stem cells

Haque, Mohammad; Xiong, Xiaofang; Lei, Fengyang; Das, Jugal Kishore; Song, Jianxun

doi:10.1016/j.xpro.2020.100264

Cited by 2 publications

(1 citation statement)

References 9 publications

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“…Alternatively, myeloid cell-derived iPSCs carrying TCR expression cassettes have overcome the hurdle of mispaired TCRαβ. Song et al also established a solid protocol for PSC-to-iT based on the OP9-DLL1/PSC monolayer co-culture system, which helped to harvest functional hepatitis B virus (HBV) Ag-specific T lymphocytes and target HBV Ag + cells in a mouse model [ 90 ]. In summary, without a well-organized thymus-like microenvironment, the designed program of T lineage from PSCs is disrupted by unpredictable factors, such as PSC-derived unfavorable cells, abnormal TCR signal, or endogenous RAG gene expression.…”

Section: Generation Of T Lymphocytes From Pscs In Vitromentioning

confidence: 99%

Generation and clinical potential of functional T lymphocytes from gene-edited pluripotent stem cells

Guo

et al. 2022

Exp Hematol Oncol

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Engineered T cells have been shown to be highly effective in cancer immunotherapy, although T cell exhaustion presents a challenge for their long-term function. Additional T-cell sources must be exploited to broaden the application of engineered T cells for immune defense and reconstitution. Unlimited sources of pluripotent stem cells (PSCs) have provided a potential opportunity to generate precise-engineered therapeutic induced T (iT) cells. Single-cell transcriptome analysis of PSC-derived induced hematopoietic stem and progenitor cells (iHSPC)/iT identified the developmental pathways and possibilities of generating functional T cell from PSCs. To date, the PSC-to-iT platforms encounter several problems, including low efficiency of conventional T subset specification, limited functional potential, and restrictions on large-scale application, because of the absence of a thymus-like organized microenvironment. The updated PSC-to-iT platforms, such as the three-dimensional (3D) artificial thymic organoid (ATO) co-culture system and Runx1/Hoxa9-enforced iT lymphopoiesis, provide fresh perspectives for coordinating culture conditions and transcription factors, which may greatly improve the efficiency of T-cell generation greatly. In addition, the improved PSC-to-iT platform coordinating gene editing technologies will provide various functional engineered unconventional or conventional T cells. Furthermore, the clinical applications of PSC-derived immune cells are accelerating from bench to bedside.

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Section: Generation Of T Lymphocytes From Pscs In Vitromentioning

confidence: 99%