“…The various parameters are computationally independent (Liao et al, 2000;Hackett et al, 2007). Such structural computations predict elements of DNA three-dimensional structure, such as local degrees of double helix flexibility versus rigidity (Ornstein et al, 1978;Brukner et al, 1995;Gorin et al, 1995;Olson et al, 1998), and have been used to predict in genomic DNA sequence biologically functional regions, including promoters and transcription start sites (Ohler et al, 2002;Wang and Benham, 2006;Abeel et al, 2008), as well as to profile insertion sites of P element transposons in Drosophila melanogaster and of retroviral and transposon vectors in transgenic animals (Liao et al, 2000;Vigdal et al, 2002;Hackett et al, 2007).…”