An optimized live imaging and growth analysis approach for Arabidopsis Sepals
Avilash Singh Yadav,
Adrienne H.K. Roeder
Abstract:Background Arabidopsis thaliana sepals are excellent models for analyzing growth of entire organs due to their relatively small size, which can be captured at a cellular resolution under a confocal microscope [1]. To investigate how growth of different tissue layers generates unique organ morphologies, it is necessary to live-image deep into the tissue. However, imaging deep cell layers of the sepal is practically challenging, as it is hindered by the presence of extracellular air spaces between mesophyll cell… Show more
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