An optimized ligation-mediated PCR method for chromosome walking and fusion gene chromosomal breakpoints identification
Jrhau Lung,
Ming-Szu Hung,
Chao-Yu Chen
et al.
Abstract:Molecular techniques that recover unknown sequences next to a known sequence region have been widely applied in various molecular studies, such as chromosome walking, identification of the insertion site of transposon mutagenesis, fusion gene partner, and chromosomal breakpoints, as well as targeted sequencing library preparation. Although various techniques have been introduced for efficiency enhancement, searching for relevant single molecular event present in a large-sized genome remains challenging. Here, … Show more
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