An Optimized Extract, Named Self-Growth Colony, from Platelet-Rich Plasma Shows Robust Skin Rejuvenation and Anti-Ageing Properties: A Novel Technology in Development of Cosmetics

Abstract

<b><i>Introduction:</i></b> Inspired by application of platelet-rich plasma (PRP) in skin treatment during injuries, an extracting method was developed here to recover high amounts of cytokines and growth factors from PRP; this prepared extract was named as self-growth colony (SGC). <b><i>Methods:</i></b> In optimization of SGC preparation, various parameters were tested, for example, centrifugation force, freeze-thaw, sonication, and inclusion of calcium chelator. The amounts of cytokines and growth factors, including platelet factor 4, β-thromboglobulin, epidermal growth factor, vascular endothelial growth factor, platelet-derived growth factor, were measured by ELISA assay. <b><i>Results:</i></b> By comparing to PRP, the prepared SGC contained a significant higher amount of measured growth factors. In addition, the degradation of growth factors within SGC during the storage was calibrated, which showed better stability as compared to that of PRP preparation. Having possible application in skin care, the optimized SGC was chemically standardized by using the enrichment of growth factors. Application of SGC in cultured keratinocytes stimulated the wound healing of injured cultures. In line to this notion, SGC was applied onto human skin, and thereafter the robust improvement of skin properties was revealed. <b><i>Conclusions:</i></b> The potential application of SGC in treating skin rejuvenation and ageing, as well as its elaborated application for medical purpose, that is, wound healing, was illustrated.

A prepared platelet‐rich plasma extract, namely Self‐Growth Colony, inhibits melanogenesis by down‐regulating microphthalmia‐associated transcription factor in skin melanocyte

“…SGC extract contains greater amount of growth factors, as well as better efficacy in skin regeneration, as comparing to conventional PRP extract. 5 To further support the application of SGC in skin regeneration, we have provided different lines of evidence to illustrate the inhibitory effect of SGC in melanogenesis. In cultured melanocyte or skin ex vivo, the applied SCG has been shown: (i) to reduce melanin production; (ii) to reduce expression of melanogenic enzymes; and (iii) to reduce the expression of the master transcription factor, MITF, in directing transcription of melanogenic enzymes.…”

“…In addition, SGC at high concentration was safe and effective in culture, ex vivo skin, and human skin. 5 Melanin synthesis takes place in melanocytes from the epidermis, which is known as the outermost layer of the skin. Melanogenesis occurs as a response to UV radiation, in which melanin is produced as a protective agent against photo-damage due to sun light.…”

“…The quantification of cytokine and growth factors in SGC was described as before. 5 ELISA kits (Abcam) detecting platelet factor-4 (PF-4), beta-thromboglobulin (β-TG), EGF, VEGF, and PDGF were deployed. Protocols attached were followed.…”

“…By optimizing the preparation method of PRP, the preparation of SGC is able to significantly increase the content of growth factors being released from platelets, and this extract is shown to have better efficacy in potentiating dermal tissue repairing, both in vitro and in vivo models. 5 Melanogenesis is defined by the production of melanin from melanocytes, leading to pigmentation of hair, eye, and skin. 6,7 Ultraviolet (UV) radiation is one of the major stimuli that induces melanogenesis in epidermal melanocytes, in which the process of melanogenesis serves as a physiological barrier of skin to protect DNA from photo-damage.…”

“…6,8,9 The application of SGC has been shown to have an excellent function in improving skin rejuvenation; however, other functions of SGC are yet to be discovered. 5 Here, we aim to reveal the novel function of SGC in anti-melanogenesis in skin cells. The expression of melanogenesis-related enzymes was determined under the influence of SGC extract.…”