An optimised phylogenetic method sheds more light on the main branching events of rhodopsin-like superfamily

Mickael, Michel‐Edwar; Rajput, Ashish; Steyn, Jannetta S.; Wiemerslage, Lyle; Bürglin, Thomas R.

doi:10.1016/j.cbd.2016.08.005

Cited by 18 publications

(21 citation statements)

References 64 publications

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“…First, we used Clustalw to align the investigated proteins in Seaview [24,25]. After that, we utilized ProtTest to determine the best amino acid replacement model as described previously [26,27]. Thirdly, we constructed the trees by using PHYML in Seaview with five random starts for each tree [28].…”

Section: Data Used and Phylogenetic Treementioning

confidence: 99%

Paracellular and Transcellular Leukocytes Diapedesis Are Divergent but Interconnected Evolutionary Events

Mickael

Kubick

Klimovich³

et al. 2021

Genes

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Infiltration of the endothelial layer of the blood-brain barrier by leukocytes plays a critical role in health and disease. When passing through the endothelial layer during the diapedesis process lymphocytes can either follow a paracellular route or a transcellular one. There is a debate whether these two processes constitute one mechanism, or they form two evolutionary distinct migration pathways. We used artificial intelligence, phylogenetic analysis, HH search, ancestor sequence reconstruction to investigate further this intriguing question. We found that the two systems share several ancient components, such as RhoA protein that plays a critical role in controlling actin movement in both mechanisms. However, some of the key components differ between these two transmigration processes. CAV1 genes emerged during Trichoplax adhaerens, and it was only reported in transcellular process. Paracellular process is dependent on PECAM1. PECAM1 emerged from FASL5 during Zebrafish divergence. Lastly, both systems employ late divergent genes such as ICAM1 and VECAM1. Taken together, our results suggest that these two systems constitute two different mechanical sensing mechanisms of immune cell infiltrations of the brain, yet these two systems are connected. We postulate that the mechanical properties of the cellular polarity is the main driving force determining the migration pathway. Our analysis indicates that both systems coevolved with immune cells, evolving to a higher level of complexity in association with the evolution of the immune system.

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Section: Data Used and Phylogenetic Treementioning

confidence: 99%

Paracellular and Transcellular Leukocytes Diapedesis Are Divergent but Interconnected Evolutionary Events

Mickael

Kubick

Klimovich³

et al. 2021

Genes

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“…They are considered as the most ancient within the class A (or rhodopsin-like) G-protein-coupled receptor (GPCR) family. Moreover, phylogenetic studies suggest that adenosine receptors were the first to start diverging [1] from the MECA receptor cluster [2] , which is formed by the melanocortin, endothelial differentiation sphingolipid, and cannabinoid receptors. There are four identified mammalian adenosine receptors (humans included) whose cognate heterotrimeric G proteins are G i (A 1 and A 3 ) or G s /G olf (A 2A and A 2B ) [3] .…”

Section: Introductionmentioning

confidence: 99%

Experimental and computational analysis of biased agonism on full-length and a C-terminally truncated adenosine A2A receptor

Navarro

González

Campanacci

et al. 2020

Computational and Structural Biotechnology Journal

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“…Interestingly, ASIP serves as anof the MC1R and the MC4R receptors. It seems melancortin system diverged from adenosine receptors around the time of divergence of Hydra vulgaris [5]. Serotonin receptors that are known to play a distinctive role in aggression seemed to have evolved as they have been found in Trichoplax adhereans [5].…”

Section: Introductionmentioning

confidence: 99%

“…It seems melancortin system diverged from adenosine receptors around the time of divergence of Hydra vulgaris [5]. Serotonin receptors that are known to play a distinctive role in aggression seemed to have evolved as they have been found in Trichoplax adhereans [5]. While dopamine receptors diverged afterwards around the time of emergence of Ciona intestinalis [5].…”

Section: Introductionmentioning

confidence: 99%

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Asip (Agouti-signaling protein) aggression gene regulate auditory processing genes in mice

Mickael¹,

Klimovich²,

Henckel³

et al. 2020

Preprint

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Covid-19 strategy of lockdown has affected the lives of millions. The strict actions to enclose the epidemic have exposed many households to inner tensions. Domestic violence has been reported to increase during the lockdown. However, the reasons for this phenomenon have not been thoroughly investigated. Melanocortin GPCRs family contribution to aggression is well documented. ASIP (nonagouti) gene plays a vital role in regulating the melanocortin GPCRs family function, and it is responsible for regulating aggression in mice. We conducted a selection analysis of ASIP. We found that it negatively purified from Shark to humans. In order to better asses the effect of this gene in mammals, we performed RNA-seq analysis of a knockout of an ASIP crisper-cas mouse model. We found that ASIP KO in mice upregulates several genes controlling auditory function, including Phox2b, Mpk13, Fat2, Neurod2, Slc18a3, Gon4l Gbx2, Slc6a3(Dat1) Aldh1a7 Tyrp1 and Lbx1. Interestingly, we found that Slc6a5, and Lamp5 as well as IL33, which are associated with startle disease, are also upregulated in response to knocking out ASIP. These findings are indicative of a direct autoimmune effect between aggression-associated genes and startle disease. Furthermore, in order to validate the link between aggression and auditory inputs processing. We conducted psychological tests of persons who experienced lockdown. We found that aggression has risen by 16 % during the lockdown. Furthermore, 3% of the subjects interviewed reported a change in their hearing abilities. Our data shed light on the importance of the auditory input in aggression and open perceptions to interpret how hearing and aggression interact at the molecular neural circuit level.

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An optimised phylogenetic method sheds more light on the main branching events of rhodopsin-like superfamily

Cited by 18 publications

References 64 publications

Paracellular and Transcellular Leukocytes Diapedesis Are Divergent but Interconnected Evolutionary Events

Paracellular and Transcellular Leukocytes Diapedesis Are Divergent but Interconnected Evolutionary Events

Experimental and computational analysis of biased agonism on full-length and a C-terminally truncated adenosine A2A receptor

Asip (Agouti-signaling protein) aggression gene regulate auditory processing genes in mice

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