An Optimised MS-Based Versatile Untargeted Metabolomics Protocol

Marques, Cátia; Justino, Gonçalo C.

doi:10.3390/separations10050314

Cited by 3 publications

(1 citation statement)

References 44 publications

(61 reference statements)

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“…Cells were cultured for 48 h and prepared for analysis as routinely for adherent cells [ 29 ]. Briefly, cells were harvested by trypsinization, washed with PBS, and centrifuged at 400× g for 5 min The pellets were stored at −80 °C overnight.…”

Section: Methodsmentioning

confidence: 99%

Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes

et al. 2023

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The growing worldwide cancer incidence, coupled to the increasing occurrence of multidrug cancer resistance, requires a continuous effort towards the identification of new leads for cancer management. In this work, two C-scorpionate complexes, [FeCl2(κ3-Tpm)] (1) and [Co(κ3-TpmOH)2](NO3)2 (2), (Tpm = hydrotris(pyrazol-1-yl)methane and TpmOH = 2,2,2-tris(pyrazol-1-yl)ethanol), were studied as potential scaffolds for future anticancer drug development. Their cytotoxicity and cell migration inhibitory activity were analyzed, and an untargeted metabolomics approach was employed to elucidate the biological processes significantly affected by these two complexes, using two tumoral cell lines (B16 and HCT116) and a non-tumoral cell line (HaCaT). While [FeCl2(κ3-Tpm)] did not display a significant cytotoxicity, [Co(κ3-TpmOH)2](NO3)2 was particularly cytotoxic against the HCT116 cell line. While [Co(κ3-TpmOH)2](NO3)2 significantly inhibited cell migration in all tested cell lines, [FeCl2(κ3-Tpm)] displayed a mixed activity. From a metabolomics perspective, exposure to [FeCl2(κ3-Tpm)] was associated with changes in various metabolic pathways involving tyrosine, where iron-dependent enzymes are particularly relevant. On the other hand, [Co(κ3-TpmOH)2](NO3)2 was associated with dysregulation of cell adhesion and membrane structural pathways, suggesting that its antiproliferative and anti-migration properties could be due to changes in the overall cellular adhesion mechanisms.

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Section: Methodsmentioning

confidence: 99%

Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes

et al. 2023

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Elucidating of the metabolic impact of risperidone on brain microvascular endothelial cells using untargeted metabolomics-based LC-MS

Ngamratanapaiboon,

Srikornvit,

Hongthawonsiri

et al. 2024

Toxicology Reports

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Exploring the Mechanisms Behind the Anti-tumoral Effects of Model C-scorpionate Complexes

Pinheiro¹,

Camões²,

Ribeiro³

et al. 2023

Preprint

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The growing worldwide cancer incidence, coupled to the increasing occurrence of multidrug cancer resistance, requires a continuous effort towards the identification of new leads for cancer management. In this work, two C-scorpionate complexes, [FeCl2(κ3-Tpm)] (1) and [Co(κ3-TpmOH)2] (2), (Tpm = hydrotris(pyrazol-1-yl)methane and TpmOH = 2,2,2-tris(pyrazol-1-yl)ethanol), are studied as potential scaffolds for future anti-cancer drug development. Their cytotoxicity and cell migration inhibitory activity are analyzed, and an untargeted metabolomics approach is em-ployed to elucidate the biological processes significantly affected by these two complexes, using two tumoral cell lines (B16 and HCT116) and a non-tumoral cell line (HaCaT). While [FeCl2(κ3-Tpm)] did not display a significant cytotoxicity, [Co(κ3-TpmOH)2] was particularly cy-totoxic against the B16 cell line. While [Co(κ3-TpmOH)2] significantly inhibited cell migration in all tested cell lines, [FeCl2(κ3-Tpm)] displayed a mixed activity. From a metabolomics perspective, exposure to [FeCl2(κ3-Tpm)] is associated with changes in various metabolic pathways involving tyrosine, where iron-dependent enzymes are particularly relevant. On the other hand, [Co(κ3-TpmOH)2] is associated with dysregulation of cell adhesion and membrane structural pathways, suggesting its antiproliferative and anti-migration properties can be due to changes in the overall cellular adhesion mechanisms.

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An Optimised MS-Based Versatile Untargeted Metabolomics Protocol

Cited by 3 publications

References 44 publications

Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes

Exploring the Mechanisms behind the Anti-Tumoral Effects of Model C-Scorpionate Complexes

Elucidating of the metabolic impact of risperidone on brain microvascular endothelial cells using untargeted metabolomics-based LC-MS

Exploring the Mechanisms Behind the Anti-tumoral Effects of Model C-scorpionate Complexes

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