An Optimal mRNA Marker for OSNA (One-step Nucleic Acid Amplification) Based Lymph Node Metastasis Detection in Colorectal Cancer Patients

Abstract

We found that CK19 mRNA has the best diagnostic performance and its cut-off value for discriminating positive from negative lymph nodes can be set in the range of 75-500 copies/µl with 96.4% sensitivity and 100% specificity.

Molecular staging of lymph node-negative colon carcinomas by one-step nucleic acid amplification (OSNA) results in upstaging of a quarter of patients in a prospective, European, multicentre study

“…These findings confirm the technical feasibility of lymph node staging of colon carcinoma patients as recently described (Croner et al , 2010; Guller et al , 2012; Yamamoto et al , 2013). OSNA appears to be a more accurate method of staging colon carcinoma patients, and identifies pN0 individuals who may benefit from adjuvant treatment.…”

“…Hence, methods are urgently needed that enable the investigation of the whole lymph node in a timely and feasible manner. OSNA is a novel molecular technology for CK19 mRNA-based identification of lymph node metastases in breast cancer and colorectal carcinomas (Tsujimoto et al , 2007; Croner et al , 2010; Yamamoto et al , 2011, 2013; Guller et al , 2012). The enormous advantage of this method is that the complete lymph node can be homogenised and transferred to OSNA without prior preparation, thus reducing the risk of potential contamination.…”

“…In contrast to current routine histopathological procedures, it enables the workup of the whole lymph node in sufficient time under clinical conditions. Lymph node metastases in breast and colorectal carcinomas can be correctly identified by this procedure (Croner et al , 2010; Cserni, 2012; Guller et al , 2012; Yamamoto et al , 2013). …”

Rapid detection of CEA mRNA in peritoneal washes using One-Step Nucleic acid Amplification (OSNA®) for gastric cancer patients

“…Previously, we and other researchers have reported a new rapid gene detection system, One-Step Nucleic acid Amplification (OSNA), for lymph node metastasis detection in breast, colorectal, gastric, and lung cancer patients [21][22][23][24]. This method consists of a simple assay sample preparation and automated rapid gene detection using a reverse transcription loop-mediated amplification (RT-LAMP) method [25].…”

Lymph Node Positivity in One-Step Nucleic Acid Amplification is a Prognostic Factor for Postoperative Cancer Recurrence in Patients with Stage II Colorectal Cancer: A Prospective, Multicenter Study

“…Subsequent studies have further demonstrated the high sensitivity of OSNA in detecting LN metastasis of breast, gastric, lung, and colorectal cancers. [9][10][11][12][13][14][15][16] Our own previous prospective, multicenter studies conducted in Japan demonstrated the usefulness of the OSNA assay as a complementary tool for diagnosing LN metastasis and CRC staging. 17,18 The later study revealed that 17.6% (13/74) of patients with histologically node-negative stage II CRC were nodal positive by the OSNA assay.…”