An optically actuated surface scanning probe

Phillips, David B.; Gibson, Graham M.; Bowman, Richard; Padgett, Miles J.; Hanna, Simon; Carberry, David M.; Miles, Mervyn J; Simpson, Stephen H.

doi:10.1364/oe.20.029679

Cited by 76 publications

(64 citation statements)

References 34 publications

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“…In the recent years the application of extended objects in indirect optical manipulation experiments is becoming more and more popular [10] owed to the possibility of the total 6 degrees of freedom motion. Their optimal trapping conditions and behavior has been extensively studied [11], [12] , and a few applications have also surfaced [13], [14], [15] . In this paper we introduce precise and fast indirect cell manipulation with extended intermediate object that is actuated by a holographic optical tweezer (HOT) and which is capable of fast 6 degrees of freedom motion.…”

Section: Introductionmentioning

confidence: 99%

High accuracy indirect optical manipulation of live cells with functionalized microtools

Vizsnyiczai

Aekbote

Búzás³

et al. 2016

SPIE Proceedings

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Optical micro manipulation of live cells has been extensively used to study a wide range of cellular phenomena with relevance in basic research or in diagnostics. The approaches span from manipulation of many cells for high throughput measurement or sorting, to more elaborated studies of intracellular events on trapped single cells when coupled with modern imaging techniques. In case of direct cell trapping the damaging effects of light-cell interaction must be minimized, for instance with the choice of proper laser wavelength. Microbeads have already been used for trapping cells indirectly thereby reducing the irradiation damage and increasing trapping efficiency with their high refractive index contrast. We show here that such intermediate objects can be tailor-made for indirect cell trapping to further increase cell-to-focal spot distance while maintaining their free and fast maneuverability. Carefully designed structures were produced with two-photon polymerization with shapes optimized for effective manipulation and cell attachment. Functionalization of the microstructures is also presented that enables cell attachment to them within a few seconds with strength much higher that the optical forces. Fast cell actuation in 6 degrees of freedom is demonstrated with the outlook to possible applications in cell imaging.

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Section: Introductionmentioning

confidence: 99%

High accuracy indirect optical manipulation of live cells with functionalized microtools

Vizsnyiczai

Aekbote

Búzás³

et al. 2016

SPIE Proceedings

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“…4 One typical example is an optically actuated surface scanning probe to investigate surface topography. 5 Another example is a hybrid nanorobot consisting of a photoresist and silicon nanowire for temperature sensing. 6 Aside from tightly focused gradient traps, counter-propagating beams can also be used in trapping.…”

Section: Introductionmentioning

confidence: 99%

Generalized phase contrast-enhanced diffractive coupling to light-driven microtools

Villangca

Bañas²,

Palima

et al. 2015

Opt. Eng

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Abstract. We have previously demonstrated on-demand dynamic coupling to optically manipulated microtools coined as wave-guided optical waveguides using diffractive techniques on a "point and shoot" approach. These microtools are extended microstructures fabricated using two-photon photopolymerization and function as freefloating optically trapped waveguides. Dynamic coupling of focused light via these structures being moved in three-dimensional space is done holographically. However, calculating the necessary holograms is not straightforward when using counter-propagating trapping geometry. The generation of the coupling spots is done in real time following the position of each microtool with the aid of an object tracking routine. This approach allows continuous coupling of light through the microtools which can be useful in a variety of biophotonics applications. To complement the targeted-light delivery capability of the microtools, the applied spatial light modulator has been illuminated with a properly matched input beam cross section based on the generalized phase contrast method. Our results show a significant gain in the output at the tip of each microtool as measured from the fluorescence signal of the trapping medium. The ability to switch from on-demand to continuous addressing with efficient illumination leverages our microtools for potential applications in stimulation and near-fieldbased biophotonics on cellular scales. © The Authors. Published by SPIE under a Creative Commons Attribution 3.0 Unported License. Distribution or reproduction of this work in whole or in part requires full attribution of the original publication, including its DOI.

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“…Using 2PP fabrication allows full 3D flexibility in the design of desired structures so that a variety of forms and tasks can be carried out. An example is the optically-actuated surface scanning probe for investigating surface topography 4 . Instead of tightly focused traps, more softly focussed counter-propagating (CP) beams can also be used in multi-beam trapping.…”

Section: Introductionmentioning

confidence: 99%

Holographic 3D tracking of microscopic tools

et al. 2015

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We originally proposed and experimentally demonstrated the targeted-light delivery capability of so-called Wave-guided Optical Waveguides (WOWs) three years ago. As these WOWs are maneuvered in 3D space, it is important to maintain efficient light coupling through their integrated waveguide structures. In this work we demonstrate the use of real-time diffractive techniques to create focal spots that can dynamically track and couple to the WOWs during operation in a volume. This is done by using a phase-only spatial light modulator to encode the needed diffractive phase patterns to generate a plurality of dynamic coupling spots. In addition, we include our proprietary GPC Light Shaper before the diffractive setup to efficiently illuminate the rectangular shaped spatial light modulator by a Gaussian laser beam. The method is initially tested for a single WOW and we have experimentally demonstrated dynamic tracking and coupling for both lateral and axial displacements of the WOWs. The ability to switch from on-demand to continuous addressing with efficient illumination leverages our WOWs for potential applications in near-field stimulation and nonlinear optics at small scales.

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An optically actuated surface scanning probe

Cited by 76 publications

References 34 publications

High accuracy indirect optical manipulation of live cells with functionalized microtools

High accuracy indirect optical manipulation of live cells with functionalized microtools

Generalized phase contrast-enhanced diffractive coupling to light-driven microtools

Holographic 3D tracking of microscopic tools

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