An Optical Multifrequency Phase-Modulation Method Using Microbeads for Measuring Intracellular Oxygen Concentrations in Plants

Schmälzlin, Elmar; Dongen, Joost T. van; Klimant, Ingo; Marmodée, Bettina; Steup, Martin; Fisahn, Joachim; Geigenberger, Peter; Löhmannsröben, Hans‐Gerd

doi:10.1529/biophysj.105.063453

Cited by 97 publications

(83 citation statements)

References 35 publications

(40 reference statements)

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“…The first indicative in-process measurements of oxygen via the use of fluorescence quenching and an optical fibre-assisted read-out system (Presense GmbH, Regensburg, Germany) is described elsewhere. 19,20 The oxygen consumption has been explored via the easy optical access to each and every position within the MOC. Another future development target, which is currently in progress, is to improve test throughput through process automation.…”

Section: Performance Of Transwell1 Multi-tissue Cultures Over 28 Daysmentioning

confidence: 99%

A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture

et al. 2013

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Dieser Beitrag ist mit Zustimmung des Rechteinhabers aufgrund einer (DFG geförderten) Allianz- bzw. Nationallizenz frei zugänglich.This publication is with permission of the rights owner freely accessible due to an Alliance licence and a national licence (funded by the DFG, German Research Foundation) respectively.Current in vitro and animal tests for drug development are failing to emulate the systemic organ complexity of the human body and, therefore, to accurately predict drug toxicity. In this study, we present a multi-organ-chip capable of maintaining 3D tissues derived from cell lines, primary cells and biopsies of various human organs. We designed a multi-organ-chip with co-cultures of human artificial liver microtissues and skin biopsies, each a 1/100 000 of the biomass of their original human organ counterparts, and have successfully proven its long-term performance. The system supports two different culture modes: i) tissue exposed to the fluid flow, or ii) tissue shielded from the underlying fluid flow by standard Transwell® cultures. Crosstalk between the two tissues was observed in 14-day co-cultures exposed to fluid flow. Applying the same culture mode, liver microtissues showed sensitivity at different molecular levels to the toxic substance troglitazone during a 6-day exposure. Finally, an astonishingly stable long-term performance of the Transwell®-based co-cultures could be observed over a 28-day period. This mode facilitates exposure of skin at the air–liquid interface. Thus, we provide here a potential new tool for systemic substance testing.BMBF, 0315569, GO-Bio 3: Multi-Organ-Bioreaktoren für die prädiktive Substanztestung im Chipforma

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Section: Performance Of Transwell1 Multi-tissue Cultures Over 28 Daysmentioning

confidence: 99%

A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture

et al. 2013

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“…The fluid flow within the micro channels was observed using a micro-ParticleImage-Velocimetry setting [14] at the marked spots in Figure b [15]. The Oxygen content can be measured and controlled by means of fluorescence decay detection [16] combined with a process gas-mixing unit [17]. This setup can be used to establish well-defined oxygen milieus in cell cultures e.g.…”

Section: Microfluidic System Developmentmentioning

confidence: 99%

Microfluidic system for enhanced cardiac tissue formation

Busek

Kolanowski

Grünzner

et al. 2017

Current Directions in Biomedical Engineering

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Hereby a microfluidic system for cell cultivation is presented in which human pluripotent stem cell-derived cardiomyocytes were cultivated under perfusion. Besides micro-perfusion this system is also capable to produce welldefined oxygen contents, apply defined forces and has excellent imaging characteristics. Cardiomyocytes attach to the surface, start spontaneous beating and stay functional for up to 14 days under perfusion. The cell motion was subsequently analysed using an adapted video analysis script to calculate beating rate, beating direction and contraction or relaxation speed.

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“…In presence of oxygen, the fluorescence lifetime of several fluorescent dyes is decreased due to quenching [14]. This decay in the fluorescence lifetime can be utilized to measure the oxygen content in the microfluidic system as shown in Figure 5 .…”

Section: Optical Oxygen Sensingmentioning

confidence: 99%

Hypoxia-on-a-chip

Busek

Grünzner

Steege

et al. 2016

Current Directions in Biomedical Engineering

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Abstract:In this work a microfluidic cell cultivation device for perfused hypoxia assays as well as a suitable controlling unit are presented. The device features active components like pumps for fluid actuation and valves for fluid direction as well as an oxygenator element to ensure a sufficient oxygen transfer. It consists of several individually structured layers which can be tailored specifically to the intended purpose. Because of its clearness, its mechanical strength and chemical resistance as well as its well-known biocompatibility polycarbonate was chosen to form the fluidic layers by thermal diffusion bonding. Several oxygen sensing spots are integrated into the device and monitored with fluorescence lifetime detection. Furthermore an oxygen regulator module is implemented into the controlling unit which is able to mix different process gases to achieve a controlled oxygenation. First experiments show that oxygenation/deoxygenation of the system is completed within several minutes when pure nitrogen or air is applied to the oxygenator. Lastly the oxygen input by the pneumatically driven micro pump was quantified by measuring the oxygen content before and after the oxygenator.

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An Optical Multifrequency Phase-Modulation Method Using Microbeads for Measuring Intracellular Oxygen Concentrations in Plants

Cited by 97 publications

References 35 publications

A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture

A dynamic multi-organ-chip for long-term cultivation and substance testing proven by 3D human liver and skin tissue co-culture

Microfluidic system for enhanced cardiac tissue formation

Hypoxia-on-a-chip

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