An optical high-pressure cell for transient grating measurements of biological substance with a high reproducibility

Hoshihara, Yuji; Kimura, Yoshifumi; Matsumoto, Masamitsu; Nagasawa, Michiyasu; Terazima, Masahide

doi:10.1063/1.2894331

Cited by 14 publications

(21 citation statements)

References 26 publications

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“…In all measurements, the internal temperature was set to be 295.5 K, and the applied pressure range was from 0.1 to 200 MPa. It has been validated that this high-pressure cell can achieve the complete reproducibility of a TG signal by applying high pressure and the sample replacement operation (39).…”

Section: Methodsmentioning

confidence: 99%

“…Details of the high-pressure apparatus used in this study have been described elsewhere (39). The pressure resistance of this cell is up to 500 MPa.…”

Section: Methodsmentioning

confidence: 99%

“…The most serious problem is ensuring the quantitative performance of the intensity of TG signals measured under the high-pressure condition. On this point, our group has developed a new high-pressure cell specially designed for TG spectroscopy (39) and overcome this problem. In this paper, by applying this high-pressure TG system to the BLUF protein TePixD, we report the first measurement, to our knowledge, of ΔK T of short-lived intermediates to investigate the mechanism underlying signal transmission by BLUF proteins, from the view point of the transient fluctuation.…”

Section: Significancementioning

confidence: 99%

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Transient conformational fluctuation of TePixD during a reaction

Kuroi

Okajima

Ikeuchi

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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Knowledge of the dynamical behavior of proteins, and in particular their conformational fluctuations, is essential to understanding the mechanisms underlying their reactions. Here, transient enhancement of the isothermal partial molar compressibility, which is directly related to the conformational fluctuation, during a chemical reaction of a blue light sensor protein from the thermophilic cyanobacterium Thermosynechococcus elongatus BP-1 (TePixD, Tll0078) was investigated in a time-resolved manner. The UV-Vis absorption spectrum of TePixD did not change with the application of high pressure. Conversely, the transient grating signal intensities representing the volume change depended significantly on the pressure. This result implies that the compressibility changes during the reaction. From the pressure dependence of the amplitude, the compressibility change of two short-lived intermediate (I 1 and I 2 ) states were determined to be +(5.6 ± 0.6) × 10 −2 cm 3 ·mol −1 ·MPa −1 for I 1 and +(6.6 ± 0.7)×10 −2 cm 3 ·mol −1 ·MPa −1 for I 2 . This result showed that the structural fluctuation of intermediates was enhanced during the reaction. To clarify the relationship between the fluctuation and the reaction, the compressibility of multiply excited TePixD was investigated. The isothermal compressibility of I 1 and I 2 intermediates of TePixD showed a monotonic decrease with increasing excitation laser power, and this tendency correlated with the reactivity of the protein. This result indicates that the TePixD decamer cannot react when its structural fluctuation is small. We concluded that the enhanced compressibility is an important factor for triggering the reaction of TePixD. To our knowledge, this is the first report showing enhanced fluctuations of intermediate species during a protein reaction, supporting the importance of fluctuations. P roteins often transfer information through changes in domaindomain (or intermolecular) interactions. Photosensor proteins are an important example. They have light-sensing domains and function by using the light-driven changes in domain-domain interactions (1). The sensor of blue light using FAD (BLUF) domain is a light-sensing module found widely among the bacterial kingdom (2). The BLUF domain initiates its photoreaction by the light excitation of the flavin moiety inside the protein, which changes the domain-domain interaction, causing a quaternary structural change and finally transmitting biological signals (3, 4). It has been an important research topic to elucidate how the initial photochemistry occurring in the vicinity of the chromophore leads to the subsequent large conformation change in other domains, which are generally apart from the chromophore.It may be reasonable to consider that the conformation change in the BLUF domain is the driving force in its subsequent reaction; that is, the change in domain-domain interaction. However, sometimes, clear conformational changes have not been observed for the BLUF domain; its conformation is very similar before and after p...

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Section: Methodsmentioning

confidence: 99%

“…Details of the high-pressure apparatus used in this study have been described elsewhere (39). The pressure resistance of this cell is up to 500 MPa.…”

Section: Methodsmentioning

confidence: 99%

Section: Significancementioning

confidence: 99%

See 1 more Smart Citation

Transient conformational fluctuation of TePixD during a reaction

Kuroi

Okajima

Ikeuchi

et al. 2014

Proc. Natl. Acad. Sci. U.S.A.

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“…conventional spectroscopy (Kimura et al 2006;Watanabe et al 2013), Fourier transform infrared spectroscopy (Dzwolak et al 2002), nuclear magnetic resonance ), small-angle neutron scattering Filabozzi et al 2010), small-angle X-ray scattering ), X-ray crystallography (Colloc'h et al 2006;, timeresolved spectroscopy (Hoshihara et al 2008), and time-resolved fluorescence anisotropy (Usui et al 2012). conventional spectroscopy (Kimura et al 2006;Watanabe et al 2013), Fourier transform infrared spectroscopy (Dzwolak et al 2002), nuclear magnetic resonance ), small-angle neutron scattering Filabozzi et al 2010), small-angle X-ray scattering ), X-ray crystallography (Colloc'h et al 2006;, timeresolved spectroscopy (Hoshihara et al 2008), and time-resolved fluorescence anisotropy (Usui et al 2012).…”

Section: Discussionmentioning

confidence: 99%

High Pressure Bioscience

Akasaka¹,

Matsuki²

2015

Subcellular Biochemistry

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The book series SUBCELLULAR BIOCHEMISTRY is a renowned and well recognized forum for disseminating advances of emerging topics in Cell Biology and related subjects. All volumes are edited by established scientists and the individual chapters are written by experts on the relevant topic. The individual chapters of each volume are fully citable and indexed in Medline/Pubmed to ensure maximum visibility of the work.

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“…Details of the high-pressure cell used for the TG measurement in this study were described elsewhere. 26 Pressure resistance of this cell was up to 500 MPa. Although the TG signal intensity is generally very sensitive to any external perturbations, such as small movement of the cell or vibration of the sample, it was confirmed that the TG signal intensity was stable and reproducible for changing pressure and the sample exchange operation.…”

Section: Experimental Methodsmentioning

confidence: 99%

Pressure-Sensitive Reaction Yield of the TePixD Blue-Light Sensor Protein

et al. 2015

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The effect of pressure on the dissociation reaction of the TePixD decamer was investigated by high-pressure transient grating (TG). The TG signal intensity representing the dissociation reaction of the TePixD decamer significantly decreased by applying a relatively small pressure. On the other hand, the reaction rate increased with increasing pressure. The equilibrium between the pentamer and the decamer was investigated by high-pressure dynamic light scattering. The results indicated that the fraction of the decamer slightly increased in the high-pressure region. From these measurements, it was concluded that the pressure-dependent signal intensity originated from the decrease of the quantum yield of the dissociation reaction of the decamer, indicating that this reaction efficiency is very sensitive to pressure. Using densimetry at high pressures, the compressibility was found to be pressure dependent even in a relatively low pressure range. We attributed the origin of the pressure-sensitive reaction yield to the decrease of compressibility at high pressure. Because the compressibility is related to the volume fluctuation, this observation suggests that the driving force for this reaction is fluctuation of the protein. The relationship between the cavities at the interfaces of the monomer units and the reactivity is also discussed.

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An optical high-pressure cell for transient grating measurements of biological substance with a high reproducibility

Cited by 14 publications

References 26 publications

Transient conformational fluctuation of TePixD during a reaction

Transient conformational fluctuation of TePixD during a reaction

High Pressure Bioscience

Pressure-Sensitive Reaction Yield of the TePixD Blue-Light Sensor Protein

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