An optical fiber based immunosensor for localized detection of IL-1β in rat spinal cord

Zhang, Kaixin; Arman, Azim; Anwer, Ayad G.; Hutchinson, Mark R.; Goldys, Ewa M.

doi:10.1016/j.snb.2018.11.054

Cited by 16 publications

(14 citation statements)

References 26 publications

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“…This means that our SS wire biosensor has the capability for cytokine detection in whole blood which is an advance over our previous study. 15 3.6. Surgical Optimization and in Vivo Detection of Intrathecal IL-1β in Rats.…”

Section: Resultsmentioning

confidence: 99%

“…14 Furthermore, the same approach was applied for cytokine detection in rat spinal cord during total dorsal laminectomy under anesthesia. 15 We also developed optical fiber based aptasensor for realtime monitoring of cytokines. 16 These previously reported devices have the capability to detect spatially localized analytes.…”

Section: Introductionmentioning

confidence: 99%

“…After capturing the IL-1β in rat spinal cord, a fluorescent readout was applied using a fluorescent detection antibody, following our earlier reports. 15 Repeatable IL-1β detection was realized in the spinal cord of live rats maintained under anesthesia. This sensing device provides a universal platform for a quantitative, spatially resolved detection of cytokines in vivo in a spectrum of health and disease states where neurokine signaling is involved.…”

Section: Introductionmentioning

confidence: 99%

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A Method for in Vivo Quantification Of Cytokine IL-1β In The Rat Intrathecal Space

Deng

Arman

Goldys

et al. 2019

ACS Appl. Bio Mater.

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IL-1β is a potent pro-inflammatory cytokine critical to multiple pathologies in the central nervous system (CNS). Quantification of IL-1β in vivo is challenging due to pM range of IL-1β released in the spinal cord and also the terminal nature of cerebrospinal fluid (CSF) sampling in rodents. Herein we developed a robust in vivo device on stainless steel suitable for detection of IL-1β in the spinal cord of rats. This approach offers high sensitivity (3.2 pg mL −1 ) and specificity to IL-1β. Also, a modified lumbar puncture method was employed to implant the device in the intrathecal space of male Sprague−Dawley (SD) rats under short-acting anesthesia, allowing minimal invasiveness, which provided the possibility of repeated measurement of IL-1β in the same animal. Our biosensing technology and the surgical method provide a universal platform for in vivo detection of diverse analytes in longitudinal, within-subject studies in the intrathecal space of rats to reduce the required number of experimental animals.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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A Method for in Vivo Quantification Of Cytokine IL-1β In The Rat Intrathecal Space

Deng

Arman

Goldys

et al. 2019

ACS Appl. Bio Mater.

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“…Measurement in-vivo was realised by introducing the immunocapture device into a perforated guide cannula in rat. Localised detection of IL-1β in the rat spinal cord was also realised using a stable immunocapture surface based on a biotinstreptavidin coupling strategy and fluorescent carboxylated supermagnetic iron oxide (SPIO)-IL-1β detection of antibody conjugates (Zhang et al, 2019). The response time and utility for continuous monitoring are key parameters to evaluate the feasibility of new affinity sensors.…”

Section: Implantable Soft and Flexible Material-based Affinity Sensorsmentioning

confidence: 99%

Soft and flexible material-based affinity sensors

Meng

Turner

Mak

2020

Biotechnology Advances

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Recent advances in biosensors and point-of-care (PoC) devices are poised to change and expand the delivery of diagnostics from conventional lateral-flow assays and test strips that dominate the market currently, to newly emerging wearable and implantable devices that can provide continuous monitoring. Soft and flexible materials are playing a key role in propelling these trends towards real-time and remote health monitoring. Affinity biosensors have the capability to provide for diagnosis and monitoring of cancerous, cardiovascular, infectious and genetic diseases by the detection of biomarkers using affinity interactions. This review tracks the evolution of affinity sensors from conventional lateral-flow assay and test strips to wearable/implantable devices enabled by soft and flexible materials. Initially, we highlight conventional affinity sensors exploiting membrane and paper materials which have been so successfully applied in point-of-care tests, such as lateral-flow immunoassay strips and emerging microfluidic paper-based devices. We then turn our attention to the multifarious polymer designs that provide both the base materials for sensor designs, such as PDMS, and more advanced functionalised materials that are capable of both recognition and transduction, such as conducting and molecularly imprinted polymers. The subsequent content discusses wearable soft and flexible material-based affinity sensors, classified as flexible and skinmountable, textile materials-based and contact lens-based affinity sensors. In the final sections, we explore the possibilities for implantable/injectable soft and flexible affinity sensors, including hydrogels, microencapsulated sensors and optical fibers. This area is truly a work in 2 progress and we trust that this review will help pull together the many technological streams that are contributing to the field.

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“…Thus, the load of antibodies on the sensor and the detection sensitivity would be affected. In order to expand the application of optical fiber immunosensors in the detection of low-level biomarkers, many studies have been devoted in recent years to improve the response of the sensors by increasing the surface area, mainly through strategies of surface modification or using porous optical fibers [ 20 , 21 , 22 , 23 , 24 , 25 , 26 , 27 , 28 , 29 , 30 , 31 , 32 , 33 , 34 ].…”

Section: Introductionmentioning

confidence: 99%

Chemiluminescent Optical Fiber Immunosensor Combining Surface Modification and Signal Amplification for Ultrasensitive Determination of Hepatitis B Antigen

Nie

Huang

et al. 2020

Sensors

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Optical fiber based immunosensors are very attractive for biomarker detection. In order to improve the sensor response, we propose a promising strategy which combines porous-layer modification of the fiber surface and streptavidin-biotin-peroxidase nano-complex signal amplification in chemiluminescent detection. Two hepatitis B antigens, hepatitis B surface antigen (HBsAg) and hepatitis B e antigen (HBeAg), are used as the targets for analysis using the proposed sensor. Comparing to immunoassays using normal optical fiber sensors, the response of the present sensor is enhanced by a factor of 4.8 and 6.7 for detection of HBsAg and HBeAg, respectively. The limit-of-quantitation of the proposed method is as low as 0.3 fg/mL (0.01 fg/mL) with a wide linear response range of 3 fg/mL–150 ng/mL (0.1 fg/mL–160 ng/mL) for sensing HBsAg (HBeAg). Quantitative determination of HBsAg and HBeAg in human serum samples is performed, showing the applicability of the proposed method for biomarker detection.

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An optical fiber based immunosensor for localized detection of IL-1β in rat spinal cord

Cited by 16 publications

References 26 publications

A Method for in Vivo Quantification Of Cytokine IL-1β In The Rat Intrathecal Space

A Method for in Vivo Quantification Of Cytokine IL-1β In The Rat Intrathecal Space

Soft and flexible material-based affinity sensors

Chemiluminescent Optical Fiber Immunosensor Combining Surface Modification and Signal Amplification for Ultrasensitive Determination of Hepatitis B Antigen

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