An optical DNA-based biosensor for the analysis of bioactive constituents with application in drug and herbal drug screening

Convection Effects in the BIAcore Dextran Layer: Surface Reaction Model

“…Varadarajan et al (2005) used the BIAcore to characterize antibody-antigen reactions in the immune response, advancing their work towards an HIV vaccine. Because of the real-time nature of the device, Minunnia et al (2005) are using the BIAcore to automate and greatly speed the process of identifying useful pharmaceutical compounds from plant samples. The speed with which the BIAcore can obtain measurements is exploited by Samsonova et al (2004) to create a preliminary screen for shellfish exposure to environmental toxins.…”

Simultaneous ligand fishing and identification of human serum albumin binders from Eucommia ulmoides bark using surface plasmon resonance-high performance liquid chromatography–tandem mass spectrometry

“…Up to now, SPR has been used as a new analytical device to fish out binders of drug targets from pure synthetic or natural molecules and natural product extracts for drug screening, such as protein tyrosine phosphatase 1b (PTP1B) binders [23], ␣-glucosidase binders [24], DNA binders [18], etc. Most ligands bind to HSA in a reversible manner by means of weak chemical bonds such as ionic, van der Waals, hydrogen and hydrophobic bonds with the hydroxyl, carboxyl or other reversible sites available in the amino acids of HSA [25].…”

“…Notwithstanding its clear advantages, SPR suffers from the inherent drawback that no structural information is generated. In this respect, SPR has been combined off-line and on-line with MS [17], off-line or in parallel with HPLC-MS [18], and on-line with HPLC-MS [19,20]. Nevertheless, the above mentioned hyphenated technologies were mostly focused on the macrobiomolecules analysis and the interfaces for on-line technology were complicated.…”

Sensitive and Label-Free Detection of DNA by Surface Plasmon Resonance

“…Carrasco et al studied the DNA sequence recognition using bispyrazinonaphthalimides antitumor agents [67]. They also probed the DNA binding properties of the indolocarbazole antitumor drug [3]. Georgiadis and coworkers demonstrated the quantitative characterization of DNA-drug interaction by angel-resolved SPRI (Fig.…”