An optical biosensor assay for the determination of folate in milk and nutritional dairy products

Determination of Folate in Infant Formula and Adult/Pediatric Nutritional Formula by Optical Biosensor Assay: First Action 2011.05

“…The results of the SLV indicate that this method is a suitable routine alternative to methods currently used to determine folate content in milk, and milk-based pediatric and adult nutritional products (2). According to previously published data, the performance of the method shows an instrumental LOD of 0.1 ng/mL (equivalent to 2.5 μg/100 g for a typical infant formula).…”

Lack of data on folate in convenience foods: Should ready-to-eat products be considered relevant for folate intake? The European challenge

“…However, as with the microbiological assay, it is not possible to distinguish between the single vitamers or show different responses to them (Arcot and Shrestha, 2005). Biosensors incorporating surface plasmon resonance (SPR) optics provide a viable analytical alternative in applications related to food composition, safety and compliance (Indyk, 2011;Lavecchia et al, 2011). The modern photosynthetic proteins-based devices, an SPR biosensor immunoassay utilising an anti-folic acid monoclonal antibody, is a viable alternative technique that unambiguously quantifies supplemental FA, due to its specificity and sensitivity to folic acid (Indyk, 2010).…”

Preparation of a new restricted access molecularly imprinted hybrid adsorbent for the extraction of folic acid from milk powder samples

“…2 The European Union (EU) recommends the daily intake of FA as being 400 mg d À1 for adults. 8,9 Therefore, owing to its reliability and excellent analytical performance for vitamin determination, HPLC is still the technique of choice for folic acid determination. 5, 6 One should note that in Brazil, lacteous products, chocolate and powder milk samples have been increasingly fortied with FA as a way of compensating the loss of vitamins during processing or simply to increase the nutritional value, once this food is classied as decient in vitamins C, D and those of the B group.…”