An operational method for the real-time monitoring of E. coli numbers in bathing waters

Lebaron, Philippe; Henry, A.; Lepeuple, A; Peña, Gisuel Consuelo Atamari; Servais, Pierre

doi:10.1016/j.marpolbul.2005.01.016

Cited by 46 publications

(45 citation statements)

References 14 publications

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“…CV decreased from 15.6% at the lowest E. coli MPN (624) to 2.6% for the highest E. coli MPN (14,136). These figures agree with the work of Lebaron who in a much more extensive study using the discontinuous 4-MUG method for measuring GUS activity reported CVs less than 15% [25]. By comparison, culture based MPN methods typically report CVs of 15% to 30% [32].…”

Section: Field Trialsupporting

confidence: 88%

“…2d. The system featured three sample chambers for performing assays in triplicate as recommended by Lebaron due to the high Coefficient of Variance (CV) (15%) of enzymatic methods [25]. Each chamber was set to incubate at 44 °C and control to within 0.5 °C.…”

Section: Prototype Design and Constructionmentioning

confidence: 99%

“…100 mL 1 as established by MPN method). Lebaron [25] using the same method reports GUS activities per culturable E. coli of approximately 20 fmol (4-MUG) min 1 per culturable E. coli for seawater samples.…”

Section: Introductionmentioning

confidence: 99%

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ColiSense, today's sample today: A rapid on-site detection of β-d-Glucuronidase activity in surface water as a surrogate for E. coli

Heery

Briciu-Burghina

Zhang

et al. 2016

Talanta

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ab s t r a c t A sensitive field-portable fluorimeter with incubating capability and triplicate sample chambers was designed and built. The system was optimised for the on-site analysis of E. coli in recreational waters using fluorescent based enzyme assays. The target analyte was β-D-Glucuronidase (GUS) which hydrolyses a synthetic substrate 6-Chloro-4-Methyl-Umbelliferyl-β-D-Glucuronide (6-CMUG) to release the fluorescent molecule 6-Chloro-4-Methyl-Umbelliferyl (6-CMU). The system was calibrated with 6-CMU standards. A LOD of 5 nM and a resolution of less than 1 nM was determined while enzyme kinetic tests showed detection of activities below 1 pmol min 1 mL 1 of sample. A field portable sample preparation, enzyme extraction protocol and continuous assay were applied with the system to analyse freshwater and marine samples. Results from a one day field trial are shown which demonstrated the ability of the system to deliver results on-site within a 75 min period.

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Section: Field Trialsupporting

confidence: 88%

Section: Prototype Design and Constructionmentioning

confidence: 99%

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ColiSense, today's sample today: A rapid on-site detection of β-d-Glucuronidase activity in surface water as a surrogate for E. coli

Heery

Briciu-Burghina

Zhang

et al. 2016

Talanta

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“…The direct measurement of b-D-galactosidase or b-D-glucuronidase activity with fluorogenic substrates has been used as rapid surrogate parameters for estimating the level of coliform bacteria and E. coli in water samples. Some studies (Caruso et al 2002;Lebaron et al 2005) showed that microbial assessments of water given by this method and the ISO standard methods were in relatively good agreement for quantification of E. coli in marine waters. However, the enzymatic results were somewhat higher than those from the cultural procedure.…”

Section: Enzymatic Methodsmentioning

confidence: 91%

“…They are capable of aerobic and facultative anaerobic growth. They ferment lactose with gas production at 35°C within 48 h and are b-galactosidase positive (Lebaron et al 2005). While some members of the coliform group are common in the intestinal tract of mammals and birds (e.g., E. coli), others occur in soil and on the surface of plants (e.g., Klebsiella, Enterobacter aerogenes, Enterobacter cloacae) and are not directly associated with fecal contamination.…”

Section: Conventional Indicators Of Fecal Contaminationmentioning

confidence: 99%

Assessment of the microbiological quality of recreational waters: indicators and methods

Rodrigues

Cunha

2017

Euro-Mediterr J Environ Integr

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The microbiological quality of recreational waters (RW) has been assessed through the use of microbial indicators. Usually, three typical fecal bacteria, namely fecal coliforms, Escherichia coli and Enterococcus spp., are deemed by legislation and regulatory agencies for routine monitoring of the fecal contamination in RW. However, these traditional indicators have some limitations, as they underestimate health risks and do not determine the sources of contamination. Currently, the increasing concern with the minimization of human health risks associated with recreational water use and the protection of water resources outweigh the actual normative perspective. The potentials and limitations of conventional and alternative fecal contamination detection systems must be carefully evaluated, taking into account the specificities of each recreational water body and the natural or anthropogenic sources of environmental variability. The careful choice of analysis for detection and quantification of indicators in the light of their potential and shortcomings is of major importance for the timely evaluation of risks and implementation of strategies that effectively protect users and preserve water quality. This paper presents a critical literature review of the traditional and innovative methodologies for the analysis of fecal indicators in recreational water and intends to discuss their contribution for the management of the microbiological quality and safety of leisure surface waters.

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Sensors for Monitoring Faecal Indicator Bacteria in Bathing Waters

Briciu-Burghina

Regan

2023

The Handbook of Environmental Chemistry

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An operational method for the real-time monitoring of E. coli numbers in bathing waters

Cited by 46 publications

References 14 publications

ColiSense, today's sample today: A rapid on-site detection of β-d-Glucuronidase activity in surface water as a surrogate for E. coli

ColiSense, today's sample today: A rapid on-site detection of β-d-Glucuronidase activity in surface water as a surrogate for E. coli

Assessment of the microbiological quality of recreational waters: indicators and methods

Sensors for Monitoring Faecal Indicator Bacteria in Bathing Waters

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