An Oomycete-Specific Leucine-Rich Repeat-Containing Protein Is Involved in Zoospore Flagellum Development in <i>Phytophthora sojae</i>

Zhang, Baiyu; Zhang, Zhichao; Yong, Saijiang; Yu, Shuyang; Feng, Hui; Yin, Mao-Zhu; Ye, Wenwu; Wang, Yuanchao; Qu, Min

doi:10.1094/phyto-12-21-0523-r

Cited by 4 publications

(3 citation statements)

References 41 publications

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“…In plants, this type of protein is well known for regulating the immune system [56]. However, LRR-containing proteins are also involved in development and pathogenicity in Phytophthora sojae [57,58]. Thus, the LRR-containing protein of BDLA may undergo selection pressure because it affects B. dothidea's development and pathogenicity.…”

Section: Discussionmentioning

confidence: 99%

The Comparative Genomics of Botryosphaeriaceae Suggests Gene Families of Botryosphaeria dothidea Related to Pathogenicity on Chinese Hickory Tree

Liang,

Jiang,

Zhang

et al. 2024

JoF

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Trunk canker poses a major threat to the production of Chinese hickory tree (Carya cathayensis Sarg.), which is primarily determined by Botryosphaeriaceae. In our previous work, we identified Botryosphaeria dothidea as the predominant pathogen of this disease. However, it is still unclear about corresponding gene families and mechanisms associated with B. dothidea’s pathogenicity on Chinese hickory tree. Here, we present a comparative analysis of high-quality genome assemblies of Botryosphaeria dothidea and other isolated pathogens, showing highly syntenic relationships between B. dothidea and its closely related species and the conservative evolution of the Botryosphaeriaceae family. Higher GC contents were found in the genomes of B. dothidea and three other isolated pathogens (Botryshaeria cortices, Botryshaeria fabicerciana, and Botryshaeria qingyuanensis) compared to Macrophomina phaseolina, Neofusicoccum parvum, Diplodia corticola, and Lasiodiplodia theobromae. An investigation of genes specific to or expanded in B. dothidea revealed that one secreted glucanase, one orsellinic acid biosynthesis enzyme, and two MFS transporters positively regulated B. dothidea’s pathogenicity. We also observed an overrepresentation of viral integrase like gene and heterokaryon incompatibility proteins in the B. dothidea’s genome. In addition, we observed one LRR-domain-containing protein and two Sec-domain-containing proteins (Sec_1 and Sec_7) that underwent positive selection. This study will help to understand B. dothidea’s pathogenicity and potential influence on the infection of Chinese hickory, which will help in the development of disease control and ensure the security of Chinese hickory production.

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Section: Discussionmentioning

confidence: 99%

The Comparative Genomics of Botryosphaeriaceae Suggests Gene Families of Botryosphaeria dothidea Related to Pathogenicity on Chinese Hickory Tree

Liang,

Jiang,

Zhang

et al. 2024

JoF

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“…The ankyrin-repeat domain (found among up-and downregulated DEGs) has a similar function [69]. Among the DEGs with cutoffs 1.5-2, downregulated genes included, e.g., two additional WD40 repeat domains and three genes with Leucine-rich repeat (whose function is largely unknown in Oomycetes but reported to be involved in zoospore flagellum development in P. sojae [70]).…”

Section: Viral Effects On the Host Gene Expression And Protein Abundancementioning

confidence: 99%

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

Poimala

Raco

Haikonen

et al. 2022

Viruses

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Phytophthora cactorum is an important oomycetous plant pathogen with numerous host plant species, including garden strawberry (Fragaria × ananassa) and silver birch (Betula pendula). P. cactorum also hosts mycoviruses, but their phenotypic effects on the host oomycete have not been studied earlier. In the present study, we tested polyethylene glycol (PEG)-induced water stress for virus curing and created an isogenic virus-free isolate for testing viral effects in pair with the original isolate. Phytophthora cactorum bunya-like viruses 1 and 2 (PcBV1 & 2) significantly reduced hyphal growth of the P. cactorum host isolate, as well as sporangia production and size. Transcriptomic and proteomic analyses revealed an increase in the production of elicitins due to bunyavirus infection. However, the presence of bunyaviruses did not seem to alter the pathogenicity of P. cactorum. Virus transmission through anastomosis was unsuccessful in vitro.

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“…In its asexual reproductive cycle, P. sojae produces zoosporangia and zoospores. Zoospores, equipped with two flagella [ 18 ], can swim in water or transition into resting spores. Notably, these zoospores exhibit a strong attraction to isoflavonoids soy glycosides released by soybean roots [ 1 , 19 ].…”

Section: Introductionmentioning

confidence: 99%

A novel method for extraction of high purity and high production Phytophthora sojae oospores

Chu,

Yin,

Yue

et al. 2024

Plant Methods

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Background Phytophthora sojae, a soil-borne oomycete pathogen, has been a yield limiting factor for more than 60 years on soybean. The resurgence of P. sojae (Phytophthora sojae) is primarily ascribed to the durable oospores found in soil and remnants of the disease. P. sojae is capable of infesting at any growth periods of the soybean, and the succeed infestation of P. sojae is predominantly attributed to long-lived oospores present in soil. Comprehending the molecular mechanisms that drive oospores formation and their significance in infestation is the key for effective management of the disease. However, the existing challenges in isolating and extracting significant quantities of oospores pose limitations in investigating the sexual reproductive stages of P. sojae. Results The study focused on optimizing and refining the culture conditions and extraction process of P. sojae, resulting in establishment of an efficient and the dependable method for extraction. Novel optimized approach was yielded greater quantities of high-purity P. sojae oospores than traditional methods. The novel approach exceeds the traditional approaches with respect to viability, survival ability, germination rates of new oospores and the pathogenicity of oospores in potting experiments. Conclusion The proposed method for extracting P. sojae oospores efficiently yielded a substantial quantity of highly pure, viable, and pathogenic oospores. The enhancements in oospores extraction techniques will promote the research on the sexual reproductive mechanisms of P. sojae and lead to the creation of innovative and effective approaches for managing oomycete diseases.

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An Oomycete-Specific Leucine-Rich Repeat-Containing Protein Is Involved in Zoospore Flagellum Development in Phytophthora sojae

Cited by 4 publications

References 41 publications

The Comparative Genomics of Botryosphaeriaceae Suggests Gene Families of Botryosphaeria dothidea Related to Pathogenicity on Chinese Hickory Tree

The Comparative Genomics of Botryosphaeriaceae Suggests Gene Families of Botryosphaeria dothidea Related to Pathogenicity on Chinese Hickory Tree

Bunyaviruses Affect Growth, Sporulation, and Elicitin Production in Phytophthora cactorum

A novel method for extraction of high purity and high production Phytophthora sojae oospores

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