An oocyte‐specific Cas9‐expressing mouse for germline CRISPR/Cas9‐mediated genome editing
Denise G. Lanza,
Jianqiang Mao,
Isabel Lorenzo
et al.
Abstract:SummaryCas9 transgenes can be employed for genome editing in mouse zygotes. However, using transgenic instead of exogenous Cas9 to produce gene‐edited animals creates unique issues including ill‐defined transgene integration sites, the potential for prolonged Cas9 expression in transgenic embryos, and increased genotyping burden. To overcome these issues, we generated mice harboring an oocyte‐specific, Gdf9 promoter driven, Cas9 transgene (Gdf9‐Cas9) targeted as a single copy into the Hprt1 locus. The X‐linked… Show more
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