An oncornavirus isolated from human cancer cell line

Ilyin, K. V.; Bykovsky, A. F.; Zhdanov, V. M.

doi:10.1002/1097-0142(197307)32:1<89::aid-cncr2820320112>3.0.co;2-i

Cited by 38 publications

(10 citation statements)

References 7 publications

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“…All other retroviruses tested in our laboratory up to now appeared to suppress the lymphocyte mitogen response (It, DE~EI~ et al, unpublished). Among the viruses tested were the bovine leukemia virus and two other type D viruses isolated from human cell lines, the HeLa-virus (2) and the HEp-2 virus (20). On the other hand, using a macrophage function test, CIA~ClOLO et al (6) found a number of viruses, including the feline leukemia virus (FeLV), the mouse mammary tumor virus and the Gibbon ape leukemia virus, which did not inhibit murine macrophages.…”

Section: Interspecies Reactivity O/pme V-induced Suppression O/lymphomentioning

confidence: 99%

“…After the isolation of the Mason-Pfizer monkey virus (MPMV) from a female rhesus monkey (9), morphologically similar viruses have been isolated from several continuous human cell lines (2,18,20). Based on electron microscopic and biochemical criteria these viruses have been classified as type D retroviruses (for review see 16,32).…”

Section: Introductionmentioning

confidence: 99%

“…So fax no oncogenic potential of MPMV (i5) and the other type D retroviruses was demonstrated and the human origin of the isolates from human cell lines (2,18,20) is still ama, tter of dispute (24). However, some immunological indications for the presence of type D retroviruses in human cancer have been reported (19).…”

Section: Introductionmentioning

confidence: 99%

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Suppression of human lymphocyte mitogen response by retroviruses of type D

Denner¹,

Indzhiia²,

Wunderlich³

et al. 1985

Archives of Virology

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In contrast to the human cell line derived type D retrovirus PMFV, the Mason-Pfizer monkey virus (MPMV) does not suppress the mitogen response of normal human lymphocytes. Both viruses have been propagated on the same cell lines and purified by the same methods. MPMV did not contain a factor able to abolish PMFV-induced suppression of the mitogen response. Neither could MPMV suppress the mitogen response of lymphocytes from rhesus monkeys or baboons. PMFV however inhibited their reactivity.

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Section: Interspecies Reactivity O/pme V-induced Suppression O/lymphomentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Suppression of human lymphocyte mitogen response by retroviruses of type D

Denner¹,

Indzhiia²,

Wunderlich³

et al. 1985

Archives of Virology

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“…However, a number of isolated reports have appeared over the last 15 years describing serological reactivity of human sera to type D virus antigens (22,34,35,44,45). In addition, a number of established human cell lines producing type D retroviruses have been described (11,16,24,25,37,47 cell line-derived type D isolates have been physically mapped with restriction endonucleases, and at least two of them have been molecularly cloned and sequenced. One cloned isolate was closely related to SRV-1 (30), and the other was very similar to the squirrel monkey retrovirus (36).…”

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confidence: 99%

Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Bohannon

Donehower

Ford

1991

J Virol

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An atypical syncytial variant of a high-grade Burkitt's-type B-cell lymphoma from a patient with AIDS who was seropositive for human immunodeficiency virus type 1 was studied. A productive type D retrovirus infection was identified in early-passage cell lines derived from two lymphomas from this patient. Nucleotide and amino acid sequence analysis as well as immunologic reactivity indicated that the isolated virus was highly related to Mason-Pfizer monkey virus (MPMV). MPMV is an immunosuppressive type D retrovirus that causes an AIDS-like syndrome in rhesus macaques. Amplification of DNA from the patient's diagnostic bone marrow biopsy specimen by polymerase chain reaction generated the appropriate MPMV-specific fragments and indicated that the patient was infected with the MPMV-like retrovirus. In addition, the patient's serum contained antibodies which recognized type D viral env proteins (gp7O and gp2O) and gag proteins (p27 and p14). Although there have been reports of human cell lines infected with type D retroviruses and of type D-reactive human sera, this is the first evidence of a type D retrovirus infection in a human confirmed by virus isolation, serum reactivity, and viral DNA identification in tumor tissue.

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“…MPMVp26 vs SMRV '%p36,RPMl 8226V,SMRV and Several laboratories demonstrated D-type virus particles in various established human cell lines (Bauer et al, 1974;Gelderblom et al, 1974;Ilyin et al, 1973;Zhdanov et al, 1973). Most of these virus particles were detected in cell lines exhibiting HeLa cell characteristics (Nelson-Rees et al, 1971) and the isolated viruses (designated also as "HeLa virus") were indistinguishable morphologically, biologically, and biochemically from Mason-Pfizer monkey virus (MPMV) (Bauer et al, 1974;Priori et al, 1976).…”

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confidence: 99%

Identification of the RPMI 8226 retrovirus and its dissemination as a significant contaminant of some widely used human and marmoset cell lines

Popovič

Kalyanaraman

Reitz

et al. 1982

Intl Journal of Cancer

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A retrovirus designated RPMI 8226V, isolated in 1973 from the human myeloma cell line RPMI 8226 has been characterized by competition radioimmunoassay (RIA) for the major viral structural protein and by nucleic acid hybridization analysis using cDNA of the virus. The virus is highly related to the squirrel monkey type D retrovirus, SMRV. In the homologous RIA using rabbit anti-RPMI 8226V and 125I-labelled p37 of RPMI 8226V, RPMI 8226V and SMRV exhibited competition of 81% and 73% respectively. Similarly, in the homologous system for SMRV p36, these viruses competed 98 and 100%. Reagents made from the type D retrovirus. Mason Pfizer Monkey Virus (MPMV), known to be related but distinct from SMRV, were used in assays designed to detect interspecies determinants of type D retroviruses. In assays using goat anti-MPMVp26 vs SMRV 125I-p36, RPMI 8226V, SMRV and MPMV competed to the same extent (93%). Hybridization analysis of RPMI 8226V cDNA showed significant homology to cellular RNA and DNA of mink, bat, and human cell infected with RPMI 8226V and to DNA or SMRV infected cells but not to uninfected cells or cells infected with other viruses. These results taken together clearly indicate that RPMI 8226V and SMRV are very closely related to each other. The finding of a type D retrovirus in this human myeloma cell line that had been used in EBV studies (the usual source of EBV being the marmoset cell line B95-8) prompted a survey of RPMI 8226V in some human and marmoset cell lines. The assays included the RIA for p36, nucleic acid hybridization using cDNA of RPMI 8226V, reverse transcriptase analysis and electron microscopy (EM). The results clearly show that in addition to RPMI 8226, human Burkitt lymphoma cells BJAB/B-95-8/K which were supertransformed by EBV from B-95-8/K marmoset cells as well as marmoset cell lines [(B-95-8/K and B-95-8/N) obtained from Stockholm and Uppsala, Sweden] were positive for the RPMI 8226V. Similar lines obtained elsewhere were negative. The results obtained clearly indicate that RPMI 8226V is a serious laboratory contamination in some widely used human cell lines. The possible impact of this viral contamination for some virological and cell biological studies is discussed.

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An oncornavirus isolated from human cancer cell line

Cited by 38 publications

References 7 publications

Suppression of human lymphocyte mitogen response by retroviruses of type D

Suppression of human lymphocyte mitogen response by retroviruses of type D

Isolation of a type D retrovirus from B-cell lymphomas of a patient with AIDS

Identification of the RPMI 8226 retrovirus and its dissemination as a significant contaminant of some widely used human and marmoset cell lines

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