Serial femtosecond crystallography (SFX) enables the determination of the room-temperature crystal structure of macromolecules without causing radiation damage and provides time-resolved molecular dynamics by pump-probe experiments. In the SFX experiment, the injector-based sample delivery method continuously provides fresh crystals to X-rays, and the fixed-target scanning method can be programmed to move the crystals to the desired location. This study introduces a combination of the inject-and-transfer system (BITS) method for sample delivery for SFX experiments, a hybrid injection, and a fixed-target scanning method. The crystal sample was embedded in a viscous lard medium and injected at a 50-100 nl/min flow rate through a syringe needle onto an ultraviolet ozone (UVO)-treated polyimide film mounted on a fixed-target scan stage. The injected sample was deposited with a width of approximately 300 μm on the film, and the loaded sample was raster scanned to XFEL by motion stage in the horizontal and vertical directions. Using this method, we successfully determined the room-temperature structure of lysozyme. This method can be applied to the SFX experiments.