An oligogenic architecture underlying ecological and reproductive divergence in sympatric populations

Brisevac, Dusica; Peralta, Carolina M; Kaiser, Tobias S

doi:10.1101/2022.08.30.505825

Cited by 3 publications

(4 citation statements)

References 49 publications

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“…Thus, if the two zeitgebers set the phase differently, losing sensitivity to one of them can be an evolutionary strategy to set the phase according to the most informative zeitgeber. In line with that, we find the same QTL locus harboring STAT-1 and CG022363 as one of the QTLs responsible for the phase-difference between Ros-2FM and Ros-2NM (manuscript submitted, Briševac et al 2022).…”

Section: Evolution Of Differential Sensitivity To Circalunar Synchron...supporting

confidence: 84%

“…However, several non-recombining regions were found in both families and one in which the marker order was inverted as compared to the reference (Figure2 B and D). These regions are thought to be large polymorphic inversions (Michailova 1980) which are limiting mapping resolution on the first chromosome and in the right arm of the second chromosome (manuscript submitted, Briševac et al 2022).…”

Section: Resultsmentioning

confidence: 99%

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Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock

Brisevac

Prakash

Kaiser

2022

Preprint

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Genetic variants underlying traits that become either non-adaptive or selectively neutral are expected to have altered evolutionary trajectories. Uncovering genetic signatures associated with phenotypic loss presents the opportunity to discover the molecular basis for the phenotype in populations where it persists. Here we study circalunar clocks in populations of marine midge Clunio marinus. The circalunar clock synchronizes development to the lunar phase, and it is set by moonlight and tidal cycles of mechanical agitation. Two out of ten studied populations have lost their sensitivity to mechanical agitation while preserving sensitivity to moonlight. Intriguingly, the F1 offspring of the two insensitive populations regained the sensitivity to mechanical entrainment, implying a genetically independent loss of the phenotype. By combining quantitative trait locus mapping and genome-wide screens, we explored the genetics of this phenotypic loss. QTL analysis suggested an oligogenic origin with one prevalent additive locus in one of the strains. In addition, it confirmed a distinct genetic architecture in the two insensitive populations. Genomic screens further uncovered several candidate genes underlying QTL regions. The strongest signal under the most prominent QTL contains a duplicated STAT1 gene, which has a well-established role in development, and CG022363, an ortholog of the Drosophila melanogaster CG32100 gene, which plays a role in gravitaxis. Our results support the notion that adaptive phenotypes have a complex genetic basis with mutations occurring at several loci. By dissecting the most prevalent signals, we started to reveal the molecular machinery responsible for the entrainment of the circalunar clock.

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Section: Evolution Of Differential Sensitivity To Circalunar Synchron...supporting

confidence: 84%

Section: Resultsmentioning

confidence: 99%

Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock

Brisevac

Prakash

Kaiser

2022

Preprint

Self Cite

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“…In whole-genome screens, core circadian clock genes have been identified as associated with the presence and absence of circasemilunar rhythms (Fuhrmann et al, 2023). The circadian clock gene period was identified as a candidate involved in determining the phase of a circalunar rhythm by a combination of QTL mapping and whole-genome screens (Briševac et al, 2023). The current study provides a mechanistic framework for those genomic findings, explaining how the circadian clock could be involved in circasemilunar time-keeping of C. marinus.…”

Section: The Functional Principle Of Clunio's Circasemilunar Clock La...mentioning

confidence: 78%

The Free-Running Circasemilunar Period Is Determined by Counting Circadian Clock Cycles in the Marine Midge Clunio Marinus

Neumann,

Rajendra,

Kaiser

2024

J Biol Rhythms

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Semilunar rhythms are found in numerous marine organisms, but the molecular mechanism and functional principles of endogenous circasemilunar clocks remain elusive. Here, we explore the connection between the free-running circasemilunar clock and the circadian clock in the marine midge Clunio marinus with three different chronobiological assays. First, we found that the free-running circasemilunar period of the adult emergence rhythm in C. marinus changes linearly with diel T-cycle length, supporting a day-counting mechanism. Second, under LD 6:6, periods of circasemilunar and circadian emergence were comparable to those under LD 12:12, indicating that the circasemilunar counter in C. marinus relies on endogenous circadian oscillations rather than external T-cycles. Finally, when desynchronizing the circadian clock with constant light, the free-running circasemilunar emergence rhythm disappeared as well, suggesting that it requires a synchronized circadian clock. These results oppose the long-held view that C. marinus’ free-running circasemilunar clock operates independently of the circadian clock. In a broader evolutionary context, our results strengthen the idea that the circasemilunar clocks of dipterous insects are based on different functional principles compared to the circasemilunar or circalunar clocks of marine annelids and algae. These divergent clock principles may indicate multiple evolutionary origins of circasemilunar and circalunar clocks.

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“…However, several non-recombining regions were found in both families and one in which the marker order was inverted as compared to the reference ( Fig 2B and 2D ). These regions are thought to be large polymorphic inversions [ 43 ] which are limiting mapping resolution on the first chromosome and in the right arm of the second chromosome [ 44 ].…”

Section: Resultsmentioning

confidence: 99%

Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock

2023

Self Cite

View full text Add to dashboard Cite

Genetic variants underlying traits that become either non-adaptive or selectively neutral are expected to have altered evolutionary trajectories. Uncovering genetic signatures associated with phenotypic loss presents the opportunity to discover the molecular basis for the phenotype in populations where it persists. Here we study circalunar clocks in populations of the marine midge Clunio marinus. The circalunar clock synchronizes development to the lunar phase, and it is set by moonlight and tidal cycles of mechanical agitation. Two out of ten studied populations have lost their sensitivity to mechanical agitation while preserving sensitivity to moonlight. Intriguingly, the F1 offspring of the two insensitive populations regained the sensitivity to mechanical entrainment, implying a genetically independent loss of the phenotype. By combining quantitative trait locus mapping and genome-wide screens, we explored the genetics of this phenotypic loss. QTL analysis suggested an oligogenic origin with one prevalent additive locus in one of the strains. In addition, it confirmed a distinct genetic architecture in the two insensitive populations. Genomic screens further uncovered several candidate genes underlying QTL regions. The strongest signal under the most prominent QTL contains a duplicated STAT1 gene, which has a well-established role in development, and CG022363, an ortholog of the Drosophila melanogaster CG32100 gene, which plays a role in gravitaxis. Our results support the notion that adaptive phenotypes have a complex genetic basis with mutations occurring at several loci. By dissecting the most prevalent signals, we started to reveal the molecular machinery responsible for the entrainment of the circalunar clock.

show abstract

An oligogenic architecture underlying ecological and reproductive divergence in sympatric populations

Cited by 3 publications

References 49 publications

Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock

Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock

The Free-Running Circasemilunar Period Is Determined by Counting Circadian Clock Cycles in the Marine Midge Clunio Marinus

Genetic analysis of a phenotypic loss in the mechanosensory entrainment of a circalunar clock

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