An olfactory receptor from Apolygus lucorum (Meyer-Dur) mainly tuned to volatiles from flowering host plants

Yan, Shuwei; Zhang, J.; Liu, Yang; Li, Guoqing; Wang, Guirong

doi:10.1016/j.jinsphys.2015.06.002

Cited by 31 publications

(37 citation statements)

References 37 publications

(39 reference statements)

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“…Five previously described ORs ( AlucOR30, AlucOR18, AlucOR12, AlucOR28 , and AlucOrco ) with completed ORF were identified again in our dataset with a high level of identity (95–100%)1831, and the remaining OR genes were named as “AlucORx” (x = 1–11, 13–17, 19–27, 29, 30–109), which was consistent with the general naming of OR genes. All the full length OR genes showed 3–8 predicted transmembrane domains and the majority of incomplete OR genes also showed multiple transmembrane domains (Table 2), which is a typical characteristic of insect OR genes, indicating that these proteins were located in the membrane of the neuron cells.…”

Section: Resultssupporting

confidence: 89%

“…In field experiments, six electrophysiological active compounds including m-xylene, butyl acrylate, butyl propionate, butyl butyrate, ( Z )-3-hexen-1-ol, and ( Z )-3-hexenyl acetate from flowering Artemisia plants were considered to be the effective substance to attract A. lucorum adults30. Moreover, an antenna highly expressed olfactory receptor gene, AlucOR28 , was identified to sensitively tune to ( Z )-3-hexenyl acetate and several flowering compounds31. Further study on the molecular mechanisms underlying the detections of these compounds is needed.…”

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confidence: 99%

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Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

Sun

Liu

et al. 2016

Sci Rep

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Olfactory receptors are believed to play a central role in insects host-seeking, mating, and ovipositing. On the basis of male and female antennal transcriptome of adult Apolygus lucorum, a total of 110 candidate A. lucorum odorant receptors (AlucOR) were identified in this study including five previously annotated AlucORs. All the sequences were validated by cloning and sequencing. Tissue expression profiles analysis by RT-PCR indicated most AlucORs were antennal highly expressed genes. The qPCR measurements further revealed 40 AlucORs were significantly higher in the antennae. One AlucOR was primarily expressed in the female antennae, while nine AlucORs exhibited male-biased expression patterns. Additionally, both the RPKM value and RT-qPCR analysis showed AlucOR83 and AlucOR21 were much higher abundant in male antennae than in female antennae, suggesting their different roles in chemoreception of gender. Phylogenetic analysis of ORs from several Hemipteran species demonstrated that most AlucORs had orthologous genes, and five AlucOR-specific clades were defined. In addition, a sub-clade of potential male-based sex pheromone receptors were also identified in the phylogenetic tree of AlucORs. Our results will facilitate the functional studies of AlucORs, and thereby provide a foundation for novel pest management approaches based on these genes.

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Section: Resultssupporting

confidence: 89%

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confidence: 99%

Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

Sun

Liu

et al. 2016

Sci Rep

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“…RNA interference (RNAi) experiments have shown that Orco is required for olfaction in A. lucorum [19]. In our previous study, we have cloned four OR genes from this species, one of which was tuned to ( Z )-3-Hexenyl acetate and several other floral compounds [20]. …”

Section: Introductionmentioning

confidence: 99%

A Female-Biased Odorant Receptor from Apolygus lucorum (Meyer-Dür) Tuned to Some Plant Odors

Zhang

Yan

et al. 2016

IJMS

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Apolygus lucorum (Meyer-Dür) (Hemiptera: Miridae) is a serious pest of cotton, jujube, grape and many other crops around the world. Understanding how olfactory information directs this insect to its host plants may provide environment-friendly approaches to the control of its population in agriculture. In our study, we cloned an odorant receptor gene, AlucOR46, that was specifically expressed in antennae and female-biased. Functional expression of AlucOR46 in Xenopus oocytes showed that it is tuned to six plant volatiles (S)-(−)-Limonene, (R)-(+)-Limonene, (E)-2-Hexenal, (E)-3-Hexenol, 1-Heptanol and (1R)-(−)-Myrtenol. Electroantennogram (EAG) recordings revealed that all six compounds could elicit electrophysiological responses from the antennae of A. lucorum, higher in females. Our results are in agreement with previous reports showing that (E)-2-Hexenal could attract female A. lucorum in behavior experiments. These results suggest that AlucOR46 might play an important role in locating the host plants of A. lucorum and therefore represents a suitable target for green pest control.

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“…However, only six ORs have been deorphanized so far. AlucOR28, 30, 40 and 46 narrowly tuned to floral scents whereas AlucOR12 and AlucOR18 did not respond to any tested odorant …”

Section: Introductionmentioning

confidence: 95%

“…Insect ORs are highly divergent seven‐transmembrane ligand‐specific proteins which have been described as heteromeric ligand‐gated ion channels and work as complexes together with a highly conserved OR called OR co‐receptor (Orco) . Since the first report of ORs in Drosophila , several methods have been developed for functional studies of ORs, of which heterologous expression in Xenopus oocyte and two‐electrode voltage‐clamp system (TEVC) have been adopted for several years . Recently, based on transcriptome and genome sequencing, a large number of insect OR repertoires have been identified .…”

Section: Introductionmentioning

confidence: 99%

Deorphanization of an odorant receptor revealed new bioactive components for green mirid bug Apolygus lucorum (Hemiptera: Miridae)

Khashaveh

Shan

et al. 2019

Pest Management Science

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BACKGROUND The Apolygus lucorum is one of the most destructive insect pests in China with a wide range of host plants. Interaction of A. lucorum with surrounding environment heavily relies on chemical communication. Deorphanization of receptors involved in odors detection elevates our understanding of the olfactory system of this pest and may help to develop a chemical ecology‐based control strategy. RESULTS AlucOR80, an odorant receptor (OR) in A. lucorum was newly cloned. Gene expression analysis showed that this receptor was mainly expressed in the antennae and head of both sexes but with a male bias. The Xenopus oocytes heterologous expression system coupled with the two‐electrode voltage‐clamp (TEVC) recording revealed that AlucOR80 was tuned to 21 selected compounds. Furthermore, electroantennogram (EAG) tests confirmed that all 21 ligands of AlucOR80 were electrophysiologically active in antennae of both sexes. Behavioral trials in a three‐cage olfactometer indicated that 16 compounds were behaviorally active, amongst which, 12 components were attractants and four components were repellents for adults of both sexes. Butyl butyrate and Dimethyl disulfide (DMDS) were the strongest attractive and repellant compounds, respectively. Importantly, we found the repellency of 1, 8‐Cineole, S‐(−)‐cis‐Verbenol and (1S)–(1)‐beta‐Pinene against adults of A. lucorum. CONCLUSION Although AlucOR80 is a general OR, may play important role in the olfactory perception of A. lucorum. Screening of AlucOR80 ligands by behavioral assay provided valuable insights by which olfactory‐based management approaches could be developed by utilizing the behaviorally active components as attractants or repellents. © 2019 Society of Chemical Industry

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An olfactory receptor from Apolygus lucorum (Meyer-Dur) mainly tuned to volatiles from flowering host plants

Cited by 31 publications

References 37 publications

Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

Identification and expression analysis of an olfactory receptor gene family in green plant bug Apolygus lucorum (Meyer-Dür)

A Female-Biased Odorant Receptor from Apolygus lucorum (Meyer-Dür) Tuned to Some Plant Odors

Deorphanization of an odorant receptor revealed new bioactive components for green mirid bug Apolygus lucorum (Hemiptera: Miridae)

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