AN ODORANT‐BINDING PROTEIN INVOLVED IN PERCEPTION OF HOST PLANT ODORANTS IN LOCUST <i>Locusta migratoria</i>

Li, Jia; Zhang, Long; Wang, Xiaoqi

doi:10.1002/arch.21319

Cited by 24 publications

(15 citation statements)

References 18 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Amongst the OBPs in this group (Group D), only the olfactory function of Lo. migratoria OBP1, which may be involved in detecting plant volatiles, has been investigated so far (Zheng et al ., ; Li et al ., ). PameOBP1 and PameOBP2 may participate in general odorant reception, having been shown to have affinities with some plant volatiles in vitro (Li et al ., ).…”

Section: Resultsmentioning

confidence: 99%

Identification of odorant‐binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana

Zhang

et al. 2017

Insect Molecular Biology

View full text Add to dashboard Cite

The American cockroach (Periplaneta americana) is an urban pest with a precise chemosensory system that helps it achieve complex physiological behaviours, including locating food and mating. However, its chemosensory mechanisms have not been well studied. Here, we identified 71 putative odorant carrier protein genes in P. americana, including 57 new odorant-binding proteins (OBPs) and 11 chemosensory proteins (CSPs). To identify their physiological functions, we investigated their tissue expression patterns in antennae, mouthparts, legs, and the remainder of the body of both sexes, and determined that most of these genes were expressed in chemosensory organs. A phylogenetic tree showed that the putative pheromone-binding proteins of P. americana were in different clades from those of moths. Two genes, PameOBP24 and PameCSP7, were expressed equally in antennae of both sexes and highly expressed amongst the OBPs and CSPs. These genes were expressed in Escherichia coli and the resultant proteins were purified. The binding affinities of 74 common odorant compounds were tested with recombinant PameOBP24 and PameCSP7. Both proteins bound a variety of ligands. Our findings provide a foundation for future research into the chemosensory mechanisms of P. americana and help in identifying potential target genes for managing this pest.

show abstract

Section: Resultsmentioning

confidence: 99%

Identification of odorant‐binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana

Zhang

et al. 2017

Insect Molecular Biology

View full text Add to dashboard Cite

show abstract

“…Similarly, another leaf volatile, Z-3-Hexenol elicited a significantly higher POR percentage over solvent (Figure 2C). Z-3-Hexenyl acetate (HA) is sensed by antenna with high sensitivity based on electroantennogram responses 28. However, similar to paraffin oil, 5% HA was unable to stimulate palps extension (Figure 2D).…”

Section: Resultsmentioning

confidence: 99%

Two Olfactory Pathways to Detect Aldehydes on Locust Mouthpart

Zhang¹,

Li²,

Zhang³

2017

Int. J. Biol. Sci.

Self Cite

View full text Add to dashboard Cite

Sensing chemical cues is crucial for insects through their olfactory systems to adapt the environments. The receptors employed in insect olfactory system belong to the Odorant Receptor (ORs) and Ionotropic Receptor (IRs) families. In general, ORs and IRs are present in distinct olfactory sensory neurons and function independently. Here, we present evidence that in locust, the abundant host plant odor Hexanal is detected by both IR- and OR-expressing neurons. Use of the palp opening response (POR) as a simple behavioral paradigm in conjunction with RNA interference (RNAi) revealed that these two pathways are both needed for the detection of Hexanal. Two-color fluorescence in situ hybridization showed that OR2 and odorant-binding protein 1 (obp1) were co-localized in palps sensilla basiconica. Obp2a and IR8a were co-localized as well, but associated with sensilla chaetica on the palps. Furthermore, both OR2- and obp1-knockdowns showed reduced POR responses to Hexanal and E-2-Hexenal, and the same was true for Hexanal with IR8a- and obp2a-knockdowns. Detection to E-2-Hexenal was independent of IR8a-mediated gene silencing. Besides, Hexanal and E-2-Hexenal evoked dose-dependent responses in palp basiconica via extracellular recordings. Our results indicate that both OR and IR pathways are involved in the detection of one aldehyde.

show abstract

“…Recently, several studies have used the RNA interference (RNAi) technique to identify OBP functions in insects (Biessmann et al, 2010; Pelletier et al, 2010; Zhang et al, 2016). Moreover, it might be possible to link behavior to OBP(s) by gene silencing (He et al, 2011; Swarup et al, 2011; Deng et al, 2013; Li et al, 2016; Shorter et al, 2016; Lin et al, 2017). In fact, RNAi based studies have shown that OBPs are involved in the detection of oviposition attractants (Biessmann et al, 2010; Pelletier et al, 2010), plant volatiles (He et al, 2011; Li et al, 2016; Zhang et al, 2016), host molecules (Deng et al, 2013), in the survival of insects (He et al, 2011; Lin et al, 2017), and regulates mating behavior (Shorter et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

et al. 2018

View full text Add to dashboard Cite

Olfactory proteins mediate a wide range of essential behaviors for insect survival. Odorant binding proteins (OBPs) are small soluble olfactory proteins involved in the transport of odor molecules (=odorants) through the sensillum lymph to odorant receptors, which are housed on the dendritic membrane of olfactory sensory neurons also known as olfactory receptor neurons. Thus, a better understanding of the role(s) of OBPs from Rhodnius prolixus, one of the main vectors of Chagas disease, may ultimately lead to new strategies for vector management. Here we aimed at functionally characterize OBPs from R. prolixus. Genes of interest were selected using conventional bioinformatics approaches and subsequent quantification by qPCR. We screened and estimated expression in different tissues of 17 OBPs from R. prolixus adults. These analyses showed that 11 OBPs were expressed in all tissues, whereas six OBP genes were specific to antennae. Two OBP genes, RproOBP6 and RproOBP13, were expressed in both male and female antennae thus suggesting that they might be involved in the recognition of semiochemicals mediating behaviors common to both sexes, such host finding (for a blood meal). Transcripts for RproOBP17 and RproOBP21 were enriched in female antennae and possibly involved in the detection of oviposition attractants or other semiochemicals mediating female-specific behaviors. By contrast, RproOBP26 and RproOBP27 might be involved in the reception of sex pheromones given that their transcripts were highly expressed in male antennae. To test this hypothesis, we silenced RproOBP27 using RNAi and examined the sexual behavior of the phenotype. Indeed, adult males treated with dsOBP27 spent significantly less time close to females as compared to controls. Additionally, docking analysis suggested that RproOBP27 binds to putative sex pheromones. We therefore concluded that RproOBP27 might be a pheromone-binding protein.

show abstract

AN ODORANT‐BINDING PROTEIN INVOLVED IN PERCEPTION OF HOST PLANT ODORANTS IN LOCUST Locusta migratoria

Cited by 24 publications

References 18 publications

Identification of odorant‐binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana

Identification of odorant‐binding and chemosensory protein genes and the ligand affinity of two of the encoded proteins suggest a complex olfactory perception system in Periplaneta americana

Two Olfactory Pathways to Detect Aldehydes on Locust Mouthpart

Functional Characterization of Odorant Binding Protein 27 (RproOBP27) From Rhodnius prolixus Antennae

Contact Info

Product

Resources

About