An Mspl and Pstl RFLP detected by probe pFMS22-1.4 [D20S22]

Abstract

Source/Description: The phage clone FMS22, containing a 0.8 and a 1.4 kb Hindlll insert, was isolated from a human chromosome 20-specific gene library (Lawrence Livermore National Laboratory, California, LL20NSO1, ATCC #57712). The 1.4 kb HindIll fragment was subcloned into the HindIII site of pBR322, giving rise to pFMS22-1.4. Polymorphisms: MspI identifies a three-allele polymorphism with bands at 4.5 (a), 2.6 (b) and 2.0 kb (c). Pst I detects two alleles with bands at 1.6 (y) and 1.1 kb (z) and an additional constant band at 1.2 kb. The DNA source is blood lymphocytes. Frequencies: MspI: a (4.5 kb) : 0.17 b (2.6 kb) : 0.46 c (2.0 kb) : 0.37 Studied in 49 unrelated European Caucasians. PIC = 0.55