An LC-MS/MS method for creatine and creatinine analysis in paraquat-intoxicated patients

Sukhang, Mukdawan; Junkuy, Anongphan; Buckley, Nicholas A.; Mohamed, Fahim; Wunnapuk, Klintean

doi:10.1080/03601234.2019.1690342

Cited by 6 publications

(5 citation statements)

References 38 publications

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“…Lao's study points to the urgent need of augmenting the existing calibration materials to creatinine measurements as low as 0.05 mg/dL. Calibration at the lower limit of detection is a problem as there is always a higher imprecision at both ends of the spectrum of measurements for a given biomarker [12]. Apart from the need for calibrated reference materials, new methodologies may have to be utilized to improve the lower limit of detection, and there may be a need to measure creatinine with novel methods necessary for precision at the lower limit of detection [12].…”

Section: Opportunities For Future Research and Translation Into Clinical Practicementioning

confidence: 99%

“…Calibration at the lower limit of detection is a problem as there is always a higher imprecision at both ends of the spectrum of measurements for a given biomarker [12]. Apart from the need for calibrated reference materials, new methodologies may have to be utilized to improve the lower limit of detection, and there may be a need to measure creatinine with novel methods necessary for precision at the lower limit of detection [12]. This is especially true in the most vulnerable population, prematurely born and term infants, who require accurate assessment of kidney function [13], not only for the diagnosis of acute kidney injury, but also for proper drug dosing of the over 60% renally excreted drugs [14].…”

Section: Opportunities For Future Research and Translation Into Clinical Practicementioning

confidence: 99%

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Still trouble with serum creatinine measurements

2022

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Section: Opportunities For Future Research and Translation Into Clinical Practicementioning

confidence: 99%

Section: Opportunities For Future Research and Translation Into Clinical Practicementioning

confidence: 99%

Still trouble with serum creatinine measurements

2022

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“…11 Various analytical techniques have been applied to detect creatinine in blood and urine. 12 Several approaches, including liquid chromatography-isotope dilution mass spectrometry, 13 surface-enhanced Raman scattering, 14 electrochemistry, 15 capillary electrophoresis, and colorimetry, 16 have been developed to detect Cn. Because several of the preceding approaches have drawbacks, such as intricate and costly materials and apparatus, it is desired to design a simple, highly sensitive, and selective Cn detection method.…”

Section: ■ Introductionmentioning

confidence: 99%

High-Sensitivity Creatinine Detection via a Dual-Emission Ratiometric Fluorescence Probe Incorporating Amino-MIL-53@Mo/ZIF-8 and Rhodamine B

Bereyhi,

Zare-Dorabei

2024

Langmuir

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Quantifying creatinine (Cn) in biological fluids is crucial for clinically assessing renal insufficiency, thyroid irregularities, and muscle damage. Therefore, it is crucial for human health to have a simple, quick, and accurate Cn analysis technique. In this study, we have successfully synthesized a 3D ratiometric dual-metal−organic framework, namely, the amino-MIL-53@Mo/ ZIF-8 and rhodamie B heterostructure, using an internal strategy for sustained growth. The dual-MOF functions as an adsorbent and preconcentrates Cn. The pH, reaction time, and volume ratio of amino-MIL-53@Mo/ZIF-8/rhodamie B were optimized using the one-variable-at-a-time technique in this study. The quantitative study of the Cn concentration for this RF biosensor was obtained under ideal conditions (R 2 = 0.9962, n = 3), encompassing the linear range of 0.35−11.1 μM. The detection and quantitation limits were 0.18 and 0.54 nM, respectively. Both intra-and interday reproducibility showed high repeatability of the RF biosensor, UV−vis, and ZETA potential studies, and the Stern−Volmer relationship was used to clarify the fluorescence quenching process. These superior sensing capabilities and the benefits of simple manufacturing, acceptable stability, and practicality make the RF biosensor intriguing for ultrasensitive Cn detection in practical applications.

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“…There are a number of reported liquid chromatography-tandem mass spectrometry (LC–MS/MS) based methods that quantify creatinine in serum 7 , 8 , plasma 9 and dried blood spots (DBS) 10 with excellent specificity and sensitivity. LC–MS/MS eliminates interferences that react with the color reagent of colorimetric assays.…”

Section: Introductionmentioning

confidence: 99%

Assessment of creatinine concentration in whole blood spheroids using paper spray ionization–tandem mass spectrometry

Sham

Badu‐Tawiah

McWilliam

et al. 2022

Sci Rep

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Accurate quantification of blood creatinine is important to estimate the glomerular filtration rate. Existing techniques using liquid chromatography tandem mass spectrometry (LC–MS/MS) have a high accuracy and eliminate most interferences encountered in routine enzymatic and Jaffé methods. However, they require laborious and time-consuming sample treatment and data acquisition. The aim of this study is to develop a fast and simple method to enable a direct analysis of whole blood creatinine with performance measures that are comparable to conventional LC–MS/MS. 5μL whole blood is formed as a three-dimensional spheroid on hydrophobic silanized paper substrates which then undergoes paper-spray ionization—tandem mass spectrometry (PSI–MS/MS). The method is validated using real human samples and compared with LC–MS/MS. PSI–MS/MS whole blood analysis exhibited a lower limit of quantification of 2.5 μg/mL, precision ≤ 6.3%, recovery in the range of 88–94% and excellent linearity (R2 > 0.99; 2.5—20 μg/mL) covering the normal range for creatinine levels. Creatinine levels were comparable to those measured by LC–MS/MS with small deviations of less than 0.3 μg/mL. This simple, fast and accurate microsampling technique for direct analysis of creatinine from whole blood shows promise for routine clinical screening and monitoring. This approach can be readily extended for other analytes of interest and, due to inherent advantages relating to cost, storability, speed, and simplicity, it can be especially advantageous for use in resource-limited settings.

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An LC-MS/MS method for creatine and creatinine analysis in paraquat-intoxicated patients

Cited by 6 publications

References 38 publications

Still trouble with serum creatinine measurements

Still trouble with serum creatinine measurements

High-Sensitivity Creatinine Detection via a Dual-Emission Ratiometric Fluorescence Probe Incorporating Amino-MIL-53@Mo/ZIF-8 and Rhodamine B

Assessment of creatinine concentration in whole blood spheroids using paper spray ionization–tandem mass spectrometry

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