An LC-MS/MS based candidate reference method for the quantification of total gentamicin in human serum and plasma using NMR characterized calibrator material

Lucha, Stephanie; Taibon, Judith; Pongratz, Stephan; Geletneky, Christian; Huber, E.M.; Wintterle-Roehm, Christine; Lang, R.; Grimm, Stefanie; Duelffer, Thomas; Tarasov, Kirill V.; Zander, Johannes; Vogeser, Michael; Kobold, Uwe

doi:10.1016/j.cca.2016.11.029

Cited by 19 publications

(10 citation statements)

References 16 publications

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“…After that, the supernatant was collected after centrifugation 12,000× g for 10 min twice. For gentamicin samples, cell lysate, in 110 µL, was added to serial dilutions of gentamicin, 20 µL internal standard solutions (Kanamycin 100 µg/mL), and 50 µL 30% trichloroacetic acid (TCA) [ 50 , 51 ]. For SA-558 samples, 2 µL of cell lysate were added to serial dilutions of SA-558 work solutions and 20 µL internal standard solutions (M21, 3.85 µg/mL).…”

Section: Methodsmentioning

confidence: 99%

Identification of a Small Molecule Compound Active against Antibiotic-Tolerant Staphylococcus aureus by Boosting ATP Synthesis

Fong

Yam

et al. 2023

IJMS

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Antibiotic tolerance poses a threat to current antimicrobial armamentarium. Bacteria at a tolerant state survive in the presence of antibiotic treatment and account for persistence, relapse and recalcitrance of infections. Antibiotic treatment failure may occur due to antibiotic tolerance. Persistent infections are difficult to treat and are often associated with poor prognosis, imposing an enormous burden on the healthcare system. Effective strategies targeting antibiotic-tolerant bacteria are therefore highly warranted. In this study, small molecule compound SA-558 was identified to be effective against Staphylococcus aureus that are tolerant to being killed by conventional antibiotics. SA-558 mediated electroneutral transport across the membrane and led to increased ATP and ROS generation, resulting in a reduction of the population of antibiotic-tolerant bacteria. In a murine chronic infection model, of which vancomycin treatment failed, we demonstrated that SA-558 alone and in combination with vancomycin caused significant reduction of MRSA abundance. Our results indicate that SA-558 monotherapy or combinatorial therapy with vancomycin is an option for managing persistent S. aureus bacteremia infection and corroborate that bacterial metabolism is an important target for counteracting antibiotic tolerance.

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Section: Methodsmentioning

confidence: 99%

Identification of a Small Molecule Compound Active against Antibiotic-Tolerant Staphylococcus aureus by Boosting ATP Synthesis

Fong

Yam

et al. 2023

IJMS

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“…LC-MS/MS was developed for total Gentamicin quantification to be used for routine assays applied for therapeutic drug monitoring of this compound. Sample preparation was based on sample dilution with an aqueous internal standard solution followed by protein precipitation [115] .…”

Section: Chromatographic Methodsmentioning

confidence: 99%

Analytical methods for the determination of certain antibiotics used in critically ill patients

Ibrahim

El-Adl

Baraka

et al. 2020

J Pharm Biopharm Res

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“…An LC-MS/MS-based RMP for the quantification of gentamicin in plasma has been published and listed by the Joint Committee for Traceability in Laboratory Medicine. 21,22 The method covers a wide range of concentrations (130-12,000 ng/mL) and shows an expected uncertainty (level of confidence 95%) of 14% at 100 ng/mL and 1.9% at 12,000 ng/mL. The composition of the calibration material for gentamicin was analyzed by nuclear magnetic resonance (NMR).…”

Section: Reference Measurement Procedures For the Detection Of Antibi...mentioning

confidence: 99%

“…Composition depends on the conditions during synthesis by bacteria. 47 If the composition of the used calibration material is known by NMR, it would be possible to achieve an improved standardization by the use of isotope dilution mass spectrometry, as described for the candidate RMP by Lucha et al 21 For this purpose, the calibration material would have to be characterized by NMR or another suitable technique. In addition, due to the complexity of gentamicin, the measurand has to be defined to ensure the comparability of an RMP using LC-MS/ MS and the immunoassays used in the routine.…”

Section: Tdm and Reference Measurement Proceduresmentioning

confidence: 99%

Quantification of Antibiotics in Patient Samples: State of the Art in Standardization and Proficiency Testing

Schneider

Heß

Keßler

et al. 2022

Therapeutic Drug Monitoring

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Background: For many antibiotics, the convenient one-fits-all dosing regimen had to be abandoned. Owing to highly variable pharmacokinetics, therapeutic drug monitoring has become an indispensable prerequisite. It is based on a suitable measuring method, sample materials, and standardization. Appropriate quality control including external quality assessment (EQA) is essential. For many antibiotics, EQAs have been established for many decades, whereas others have only lately been introduced. This article gives an insight into the state of the art regarding the therapeutic drug monitoring of antibiotics regarding standardization, EQAs, and reference measurement procedures (RMPs).Methods: An overview of the currently available international EQA schemes for antibiotics and a literature overview of available RMPs are given. EQAs including gentamicin and vancomycin have been offered by German providers for more than 25 years. The period 2000-2020 was selected for a detailed analysis. The experiences with a new EQA including linezolid, meropenem, and piperacillin are described.Results: EQAs for gentamicin and vancomycin are provided in many countries. Those for linezolid, meropenem, and piperacillin do not seem to be very common. Most of the antibiotics monitored for decades are measured by commercially available assays. EQAs for linezolid, meropenem, and piperacillin introduced in 2018 were rapidly accepted in Germany. Methods reported in this study were HPLC based either with UV or mass spectrometric detection. The number of participants succeeding was comparable between UV and mass spectrometry. Candidate RMPs for gentamicin, vancomycin, and linezolid based on isotope dilution mass spectrometry were published. Conclusions:EQAs are offered regularly for many antibiotics worldwide. The results of EQAs in Germany generally compare well, but there is potential for improvement. Both immunoassays and HPLC-based methods work properly in EQAs evaluated in Germany. From a quality control perspective, fast and inexpensive methods may be selected without endangering the patient's health based on clinical needs.

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An LC-MS/MS based candidate reference method for the quantification of total gentamicin in human serum and plasma using NMR characterized calibrator material

Cited by 19 publications

References 16 publications

Identification of a Small Molecule Compound Active against Antibiotic-Tolerant Staphylococcus aureus by Boosting ATP Synthesis

Identification of a Small Molecule Compound Active against Antibiotic-Tolerant Staphylococcus aureus by Boosting ATP Synthesis

Analytical methods for the determination of certain antibiotics used in critically ill patients

Quantification of Antibiotics in Patient Samples: State of the Art in Standardization and Proficiency Testing

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