An LC Method for the Determination of Bupropion and Its Main Metabolite, Hydroxybupropion in Human Plasma

Yeniceli, Duygu; Doğrukol-Ak, Dilek

doi:10.1365/s10337-009-1361-z

Cited by 22 publications

(16 citation statements)

References 16 publications

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“…The extraction methods of BUP and its metabolites from biological matrix include liquid-liquid extraction (LLE) [16–18], solid phase extraction (SPE) [11, 14, 19] and protein precipitation (PP) [12,22]. In this investigation, LLE and PP methods were used.…”

Section: Resultsmentioning

confidence: 99%

“…Therefore, the combination of acetonitrile and TCA was used instead, which resulted in high recovery for all four analytes in plasma (~89–96%, see Table 5). In the previous reports [12, 22], 3–4% TCA (w/v) was used for rat plasma protein precipitation for the determination of BUP and OH-BUP. However, TCA acts as an ion-pairing reagent in the chromatographic method [23] and its final concentration in the samples had a significant effect on the retention time of BUP and its metabolites.…”

Section: Resultsmentioning

confidence: 99%

“…Analytical methods using liquid chromatography for separation of the drug and metabolites and detection by UV (LC-UV) at 254 nm (for bupropion) and 214 nm (for its metabolites), mass spectrometry (LC-MS) or LC-MS/MS have been developed and validated for quantitative determination of BUP and its major metabolite OH-BUP in human plasma [11,12,13,14,15] and urine [14]. Simultaneous quantitative determination of BUP and all metabolites in plasma using LC-MS/MS method coupled with monolithic column [16] or LC-UV [17,18], and in whole blood using ultra-LC-MS/MS [19] have been also reported.…”

Section: Introductionmentioning

confidence: 99%

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Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography–tandem mass spectrometry

Wang¹,

Vernikovskaya²,

Abdelrahman³

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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A liquid chromatography in tandem with electro-spray ionization mass spectrometry method has been developed and validated for the quantitative determination of BUP and its major metabolites (hydroxybupropion, threo- and erythrohydrobupropion) in human umbilical cord plasma and placental tissue. The samples were acidified with trichloroacetic acid, and protein precipitated by adding acetonitrile. Chromatographic separation of drug and metabolites was achieved by using a Waters Symmetry C18 column, with an isocratic elution of 31% methanol and 69% formic acid (0.04%, v/v) aqueous solution at a flow rate of 1.0 mL/min. Detection was carried out by mass spectrometry using positive electro-spray ionization mode, and the compounds were monitored using multiple reactions monitoring method. Deuterium-labeled isotopes of the compounds were used as internal standards. Calibration curves were linear (r2 >0.99) in the tested ranges. The lower limit of quantification of analytes in umbilical cord plasma samples is < 0.72 ng/mL and 0.92 ng/g in placental tissue samples. The relative deviation of this method was < 15% for intra- and inter-day assays, and the accuracy ranged between 88 and 105%. The extraction recovery of the four analytes ranged between 89 and 96% in umbilical cord plasma, and 64 and 80% in placental tissue. No significant matrix effect was observed in the presented method.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography–tandem mass spectrometry

Wang¹,

Vernikovskaya²,

Abdelrahman³

et al. 2012

Journal of Pharmaceutical and Biomedical Analysis

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“…The analysis of BUP in biological fluids [6][7][8][9][10][11][12] and in pharmaceuticals has been performed with liquid chromatography, liquid chromatography-mass spectrometry electrokinetic chromatography and thin layer chromatography [13][14][15] . Also, several studies including chiral separations of BUP and HBUP enantiomers were described [16][17][18] .…”

Section: Research Articlementioning

confidence: 99%

Adsorptive Stripping Voltammetric Determination of Bupropion in Pharmaceuticals

2017

Chem Sci Trans

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Electrochemical studies of bupropion by voltammetric techniques were carried out. The bupropion gave one well-defined reduction peak at -1.2 to -1.3V vs. Ag/AgCl electrode in Britton Robinson buffer BR of pH 9. Under optimized conditions, a linear response was obtained between 1.0x10 -6 M to 5.6x10 -6 M in aqueous media for square wave and differential pulse cathodic adsorptive stripping voltammetric techniques. The electrochemical process was diffusion controlled. The value of limit of detection (LOD) are 1.28x10

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“…graphy (LC) [13][14][15], liquid chromatography mass spectrometry (LC-MS) [16], liquid chromatography--tandem mass spectrometry (LC-MS/MS) [17][18][19], liquid chromatography electrospray ionization-tandem mass spectrometry (LC/ESI/MS/MS) [20], capillary electrophoresis [21] and radioimmunoassay [22]. Several methods have been reported for the determination of BUPH in pharmaceuticals such as non--aqueous titration [23], HPLC [23][24][25][26], gas liquid chromatography (GLC) [27], LC [28], thin-layer chromatography (TLC) [29], capillary electrophoresis [21], polarography [30], potentiometry [31], conductometry [31], UV-spectrophotometry [31,32] and visible spectrophotometry [33,34].…”

mentioning

confidence: 99%

Application of ion-association titration for the assay of bupropion hydrochloride in pharmaceuticals

Abdulrahman

Basavaiah

2011

CI&CEQ

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Two simple and rapid titrimetric methods are described for the determination of bupropion hydrochloride (BUPH) in pharmaceuticals. The proposed methods are based on the solvent extraction-titration of BUPH with two ion-association reagents, i.e., sodium lauryl sulphate (SLS) and tetraphenylborate (TPB). In method A, SLS was used as titrant and the titration was carried out in the presence of dilute sulphuric acid and chloroform using dimethyl yellow as indicator whereas in method B, the titrant was TPB and the titration was done in borate-phosphate buffer of pH 6.0 and 1,2-dichloroethane with tetrabromophenolphthalein ethyl ester (TBPE) as indicator. In the two-phase titrations, the proposed procedures give sharp end points as the color of the organic phase changes from yellow to pink in method A and from red-violet to yellow in method B. The methods are applicable over the ranges of 1.0-20.0 and 3.0-10.0 mg of BUPH for methods A and B, respectively. The accuracy and precision of the methods are good. The methods were applied successfully to the determination of BUPH in tablets and the results were in agreement with the label claim and those of the comparison method.

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An LC Method for the Determination of Bupropion and Its Main Metabolite, Hydroxybupropion in Human Plasma

Cited by 22 publications

References 16 publications

Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography–tandem mass spectrometry

Simultaneous quantitative determination of bupropion and its three major metabolites in human umbilical cord plasma and placental tissue using high-performance liquid chromatography–tandem mass spectrometry

Adsorptive Stripping Voltammetric Determination of Bupropion in Pharmaceuticals

Application of ion-association titration for the assay of bupropion hydrochloride in pharmaceuticals

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