An L012@PAni-PAAm hydrogel composite based-electrochemiluminescence biosensor for in situ detection of H2O2 released from cardiomyocytes

Guo, Xiaojin; Li, Yabei; Li, Yingchun; Ye, Zhaoyang; Zhang, Junjie; Zhu, Tong; Li, Fēi

doi:10.1016/j.electacta.2020.136763

Cited by 28 publications

(22 citation statements)

References 63 publications

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“…Then, the H 2 O 2 oxidation current reduced to its initial value within 15 min, which was attributed to the rebalance of the cellular homeostatic system, for example, nicotinamide adenine dinucleotide phosphate, thioredoxin, and glutathione. , For the cardiac tissue treated with propranolol, except for the fluctuation at 15 min due to the addition of propranolol in the solution, no significant change in current was observed, suggesting that the addition of propranolol would not induce H 2 O 2 generation from the cardiac tissue within 30 min. The possible reason for this phenomenon is that the average concentration of H 2 O 2 in cardiac tissues under normal physiological is at nanomolar ranges. , After the activation of β-receptors of cardiomyocytes by isoproterenol, mitochondrial oxidative phosphorylation is enhanced to meet the ATP demand of cardiac tissues with promoted metabolic activity, in which the H 2 O 2 secretion from the cardiac tissues increases. Meanwhile, for the cardiac tissues treated with propranolol, the β-receptors of cardiomyocytes is blocked, which induces the decrease of the metabolic activity of the cardiac tissues, further reducing the release of H 2 O 2 .…”

Section: Resultsmentioning

confidence: 99%

“…For the cardiac tissue treated with propranolol, except for the fluctuation at 15 min due to the addition of propranolol in the solution, no significant change in current was observed, suggesting that the addition of propranolol would not induce H 2 O 2 generation from the cardiac tissue within 30 min. The possible reason for this phenomenon is that the average concentration of H 2 O 2 in cardiac tissues under normal physiological is at nanomolar ranges 51,52. After the activation of β-receptors of cardiomyocytes by isoproterenol, mitochondrial oxidative phosphorylation is enhanced to meet the ATP demand of cardiac tissues with promoted metabolic activity, in which the H 2 O 2 secretion from the cardiac tissues increases.…”

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confidence: 99%

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In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

Zhang

et al. 2022

Anal. Chem.

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In vitro cardiac tissue model holds great potential as a powerful platform for drug screening. Respiratory activity, contraction frequency, and extracellular H2O2 levels are the three key parameters for determining the physiological functions of cardiac tissues, which are technically challenging to be monitored in an in situ and quantitative manner. Herein, we constructed an in vitro cardiac tissue model on polyacrylamide gels and applied a pulsatile electrical field to promote the maturation of the cardiac tissue. Then, we built a scanning electrochemical microscopy (SECM) platform with programmable pulse potentials to in situ characterize the dynamic changes in the respiratory activity, contraction frequency, and extracellular H2O2 level of cardiac tissues under both normal physiological and drug (isoproterenol and propranolol) treatment conditions using oxygen, ferrocenecarboxylic acid (FcCOOH), and H2O2 as the corresponding redox mediators. The SECM results showed that isoproterenol treatment induced enhanced oxygen consumption, accelerated contractile frequency, and increased released H2O2 level, while propranolol treatment induced dynamically decreased oxygen consumption and contractile frequency and no obvious change in H2O2 levels, suggesting the effects of activation and inhibition of β-adrenoceptor on the metabolic and electrophysiological activities of cardiac tissues. Our work realizes the in situ and quantitative monitoring of respiratory activity, contraction frequency, and secreted H2O2 level of living cardiac tissues using SECM for the first time. The programmable SECM methodology can also be used to real-time and quantitatively monitor electrochemical and electrophysiological parameters of cardiac tissues for future drug screening studies.

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Section: Resultsmentioning

confidence: 99%

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confidence: 99%

In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

Zhang

et al. 2022

Anal. Chem.

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“…[26] Qualitative cell viability can be determined by bright-field and scanning electron microscopy to observe uniform adhesion of cells on the polymer fibers. [20,27] Spindle formation, pseudopodia extension, flattened cells, and spreading morphologies demonstrate high adhesion to the polymer network, indicating that cells are exploring the space and entering the porous structures to increase the area of detection (Figure 2a). [20,28] Fluorescent imaging using double-stained experiments can demonstrate cytotoxicity by determining relative numbers of live to dead cells.…”

Section: Biocompatibility Cell Viability and Cytotoxicitymentioning

confidence: 99%

Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Shafique

Vries

Strauss

et al. 2022

Adv Healthcare Materials

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Novel biomaterials for bio‐ and chemical sensing applications have gained considerable traction in the diagnostic community with rising trends of using biocompatible and lowly cytotoxic material. Hydrogel‐based electrochemical sensors have become a promising candidate for their swellable, nano‐/microporous, and aqueous 3D structures capable of immobilizing catalytic enzymes, electroactive species, whole cells, and complex tissue models, while maintaining tunable mechanical properties in wearable and implantable applications. With advances in highly controllable fabrication and processability of these novel biomaterials, the possibility of bio‐nanocomposite hydrogel‐based electrochemical sensing presents a paradigm shift in the development of biocompatible, “smart,” and sensitive health monitoring point‐of‐care devices. Here, recent advances in electrochemical hydrogels for the detection of biomarkers in vitro, in situ, and in vivo are briefly reviewed to demonstrate their applicability in ideal conditions, in complex cellular environments, and in live animal models, respectively, to provide a comprehensive assessment of whether these biomaterials are ready for point‐of‐care translation and biointegration. Sensors based on conductive and nonconductive polymers are presented, with highlights of nano‐/microstructured electrodes that provide enhanced sensitivity and selectivity in biocompatible matrices. An outlook on current challenges that shall be addressed for the realization of truly continuous real‐time sensing platforms is also presented.

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“…The combination of the transient nature and matrix effects of H 2 O 2 makes monitoring this molecule in biological samples a challenge. In many current studies, most of the detection of H 2 O 2 is to make the cells into a suspension, which undoubtedly increases the diffusion distance of H 2 O 2 between the cell and the sensing interface. − In situ culture on the cells on electrodes can effectively shorten the electron transfer process transfer between the cells and electrodes. An ideal electrochemical bioplatform for the in situ detection of H 2 O 2 should possess excellent catalytic performance, accurate selectivity, good biocompatibility, and a quasiphysiological environment favorable for cell growth. − Carbon fiber cloth (CFC) with microscale size and fascinating mechanical properties can be selected as a rich and flexible microelectrode substrate in the electrochemical biosensor system − in which the ordered nanoarray with unique physical and chemical properties serves as “electronic wires”, effectively enhancing the electron transfer between the electrodes and cells. , However, bare CFC has poor catalytic ability, so it often needs to be combined with other nanomaterials.…”

Section: Introductionmentioning

confidence: 99%

Au and Pt Nanoparticles Grown on Flexible Carbon Fiber Cloth Supports Decorated with Cerium Metal Organic Frameworks for the Real-Time Detection of H₂O₂ in Live Cancer Tissue

Yang

Dong

et al. 2022

ACS Appl. Nano Mater.

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Flexible electrochemical biosensors have ignited great interest because they possess the potential to provide continuous and real-time physiological information via dynamic and noninvasive measurements of biochemical markers in biofluids. In this work, a cerium metal−organic framework (Ce-UiO-66-4,4-biphenyl dicarboxylic acid (BPDC)) was first grown in situ on flexible carbon fiber cloth (CFC) by a one-step hydrothermal method. To further shorten the response time of the biosensor, we used the controllable growth of gold nanoparticles (NPs) and the peroxidase activity of platinum NPs to simultaneously deposit Au and Pt NPs on the surface of Ce-UiO-66-BPDC/CFC, and constructed a supersensitive sensing interface with good electrocatalytic activity. Under the optimal experimental conditions, the detection limit of the enzyme-free H 2 O 2 electrochemical sensor was as low as 89 nM (S/N = 3). Moreover, due to the excellent biocompatibility and flexibility of the Au−Pt/Ce-UiO-66-BPDC/CFC (APCC) bioplatform, lung cancer cells (A549) can be directly cultured on the electrodes to achieve real-time monitoring of the H 2 O 2 released by living cells (2.43 × 10 −14 mol cell −1 ). More importantly, the practical application of APCC fiber microelectrodes explored the real-time monitoring of the release of H 2 O 2 from fresh tissues surgically excised from breast, breast cancer, and lung cancer, which provided an advanced tool for further research on reactive oxygen species biology, such as oxidative stress and subsequent pathology.

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An L012@PAni-PAAm hydrogel composite based-electrochemiluminescence biosensor for in situ detection of H2O2 released from cardiomyocytes

Cited by 28 publications

References 63 publications

In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Au and Pt Nanoparticles Grown on Flexible Carbon Fiber Cloth Supports Decorated with Cerium Metal Organic Frameworks for the Real-Time Detection of H₂O₂ in Live Cancer Tissue

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An L012@PAni-PAAm hydrogel composite based-electrochemiluminescence biosensor for in situ detection of H2O2 released from cardiomyocytes

Cited by 28 publications

References 63 publications

In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

In Situ and Quantitative Monitoring of Cardiac Tissues Using Programmable Scanning Electrochemical Microscopy

Advances in the Translation of Electrochemical Hydrogel‐Based Sensors

Au and Pt Nanoparticles Grown on Flexible Carbon Fiber Cloth Supports Decorated with Cerium Metal Organic Frameworks for the Real-Time Detection of H2O2 in Live Cancer Tissue

Contact Info

Product

Resources

About

Au and Pt Nanoparticles Grown on Flexible Carbon Fiber Cloth Supports Decorated with Cerium Metal Organic Frameworks for the Real-Time Detection of H₂O₂ in Live Cancer Tissue