An Isolated Working Heart System for Large Animal Models

Schechter, Matthew A.; Southerland, Kevin W.; Feger, Bryan J.; Linder, Dean; Ali, Ayyaz; Njoroge, Linda; Milano, Carmelo A.; Bowles, Dawn E.

doi:10.3791/51671-v

Cited by 6 publications

(1 citation statement)

References 17 publications

(38 reference statements)

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“…The RA was sutured closed so that the venous return from the coronary sinus was ejected through the pulmonary artery only, which was cannulated to monitor coronary blood flow (CBF). The entire heart was then quickly removed from the storage container, pouring out any storage solution still in the ventricles, and weighed (19). The heart was oriented in the ESHP system so that aorta was facing upward with the posterior aspect of the heart resting on a plastic sheet, which was part of our custom blood collection system.…”

Section: Heart Harvesting and Preparationmentioning

confidence: 99%

A Multi-Mode System for Myocardial Functional and Physiological Assessment during Ex Situ Heart Perfusion

Duignan,

Guariento,

Doulamis

et al. 2020

J Extra Corpor Technol

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Ex situ heart perfusion (ESHP) has proven to be an important and valuable step toward better preservation of donor hearts for heart transplantation. Currently, few ESHP systems allow for a convenient functional and physiological evaluation of the heart. We sought to establish a simple system that provides functional and physiological assessment of the heart during ESHP. The ESHP circuit consists of an oxygenator, a heart–lung machine, a heater–cooler unit, an anesthesia gas blender, and a collection funnel. Female Yorkshire pig hearts (n = 10) had del Nido cardioplegia (4°C) administered, excised, and attached to the perfusion system. Hearts were perfused retrogradely into the aortic root for 2 hours before converting the system to an isovolumic mode or a working mode for further 2 hours. Blood samples were analyzed to measure metabolic parameters. During the isovolumic mode (n = 5), a balloon inserted in the left ventricular (LV) cavity was inflated so that an end-diastolic pressure of 6–8 mmHg was reached. During the working mode (n = 5), perfusion in the aortic root was redirected into left atrium (LA) using a compliance chamber which maintained an LA pressure of 6–8 mmHg. Another compliance chamber was used to provide an afterload of 40–50 mmHg. Hemodynamic and metabolic conditions remained stable and consistent for a period of 4 hours of ESHP in both isovolumic mode (LV developed pressure: 101.0 ± 3.5 vs. 99.7 ± 6.8 mmHg, p = .979, at 2 and 4 hours, respectively) and working mode (LV developed pressure: 91.0 ± 2.6 vs. 90.7 ± 2.5 mmHg, p = .942, at 2 and 4 hours, respectively). The present study proposed a novel ESHP system that enables comprehensive functional and metabolic assessment of large mammalian hearts. This system allowed for stable myocardial function for up to 4 hours of perfusion, which would offer great potential for the development of translational therapeutic protocols to improve dysfunctional donated hearts.

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Section: Heart Harvesting and Preparationmentioning

confidence: 99%

A Multi-Mode System for Myocardial Functional and Physiological Assessment during Ex Situ Heart Perfusion

Duignan,

Guariento,

Doulamis

et al. 2020

J Extra Corpor Technol

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Ex Vivo Working Porcine Heart Model

Pigot,

Soltesz,

Steen

2024

Methods in Molecular Biology

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Ex vivo working porcine heart models allow for the study of a heart’s function and physiology outside the living organism. These models are particularly useful due to the anatomical and physiological similarities between porcine and human hearts, providing an experimental platform to investigate cardiac disease or assess donor heart viability for transplantation. This chapter presents an in-depth discussion of the model’s components, including the perfusate, preload, and afterload. We explore the challenges of emulating cardiac afterload and present a historical perspective on afterload modeling, discussing various methodologies and their respective limitations. An actively controlled afterload device is introduced to enhance the model’s ability to rapidly adjust pressure in the large arteries, thereby providing a more accurate and dynamic experimental model. Finally, we provide a comprehensive experimental protocol for the ex vivo working porcine heart model.

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