An isolated skeletal muscle model suitable for acute ischemia studies

Kuzon, William M.; Walker, Paul; Mickle, Donald A.G.; Harris, Kristin; Pynn, Bruce R.; McKee, Nancy H.

doi:10.1016/0022-4804(86)90004-1

Cited by 54 publications

(20 citation statements)

References 16 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…The extent of skeletal muscle necrosis in complete ischaemia increases with the length of ischaemic period [21]. Using an isolated muscle model, Kuzon et al [22] concluded that 7 h of ischaemia caused irreversible muscle damage. Harris et al [14], using a gracilis model, reported that muscle necrosis occurred after 4 h and became extensive by 7 h. Consistently, in our model, oedema of ischaemic muscle fibres occurred almost immediately after complete ischaemia and progressed over time.…”

Section: Discussionmentioning

confidence: 99%

Skeletal muscle evaluation by MRI in a rabbit model of acute ischaemia

Zhang¹,

Wang²,

Guan³

et al. 2013

BJR

View full text Add to dashboard Cite

Objective: To assess rhabdomyolysisassociated skeletal muscle changes induced by complete ischaemia in rabbits using MRI.Methods: Acute ischaemia was induced in the right hind limb of 34 New Zealand white rabbits by arterial ligation. MRI of vastus lateralis was carried out pre-operatively and every hour post-operatively up to 7 h. T 1 weighted images, T 2 weighted images with fat suppression, T 2 maps and diffusion tensor scans were obtained. The correlation of MRI findings with histopathological changes in biopsies of vastus lateralis was examined.Results: Histopathology demonstrated early cellular oedema 1 h post ischaemia and irreversible injuries by 7 h, including loss of striation and broken muscle fibres. T 2 weighted images with fat suppression showed inhomogeneous high signal intensity of vastus lateralis, which progressively increased from 2 h following ischaemia. The T 2 relaxation rate of ischaemic vastus lateralis was significantly greater than normal muscle (p,0.001) and demonstrated a linear increase with time following ischaemia. A similar linear increase was also found in the ischaemic vastus lateralis apparent diffusion coefficient (ADC) 1-5 h post ischaemia (p50.006). Both the T 2 ADC and fractional anisotropy (FA) were significantly higher on the ischaemic side 7 h post ischaemia (for T 2 , p50.02; for ADC, p50.004). Conclusion:Muscle oedema is detectable on MR images and is reflected well by T 2 , ADC and FA values. MRI may have value in clinical evaluation of rhabdomyolysis. Advances in knowledge:Ischaemic changes detected by MRI may have value in the diagnosis of rhabdomyolysis.

show abstract

Section: Discussionmentioning

confidence: 99%

Skeletal muscle evaluation by MRI in a rabbit model of acute ischaemia

Zhang¹,

Wang²,

Guan³

et al. 2013

BJR

View full text Add to dashboard Cite

show abstract

“…Isolated paired nonheparinized in vivo gracilis muscle flaps were prepared as described. 22,23 Briefly, a linear incision was made in the skin over the gracilis muscle. The muscle was dissected from its proximal attachment to the pubis and its distal insertion on the medial aspect of the tibia.…”

Section: Methodsmentioning

confidence: 99%

Detection of free radicals in ischemic and reperfused canine gracilis muscle flaps by use of spin-trapping electron paramagnetic resonance spectroscopy

Ba¹,

Cw²,

Chen³

et al. 2001

ajvr

View full text Add to dashboard Cite

Free radicals may be an important component of injury induced by ischemia and reperfusion of canine skeletal muscle. Spin-trap adducts of free radicals can be detected in effluent blood of canine muscle flaps by use of spin-trapping EPR spectroscopy. Spin-trapping EPR spectroscopy may be useful for the study of antioxidants and free radical scavengers in attenuating ischemia and reperfusion-mediated skeletal muscle damage.

show abstract

“…Clinically, embolic disease, operating tourniquet use, were performed in adult rat hindlimbs. MHC expresor microneurovascular muscle transplantation can resion during skeletal muscle regeneration was detersult in prolonged muscle ischemia with muscle cell nemined sequentially using immunocytochemistry.crosis and subsequent muscle fiber regeneration [1][2][3][4]. …”

mentioning

confidence: 99%