An isolated<i>Amycolatopsis</i>sp. GDS for cellulase and xylanase production using agricultural waste biomass

Kshirsagar, Siddheshwar D.; Saratale, Ganesh Dattatraya; Saratale, Rijuta Ganesh; Govindwar, Sanjay P.; Oh, Min Kyu

doi:10.1111/jam.12988

Cited by 36 publications

(13 citation statements)

References 42 publications

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“…After treatment with Y7, the length and width of the switchgrass were substantially decreased. Similar structural changes were also observed in the hydrolyzed wheat straw (Kshirsagar et al, 2016) and switchgrass (Wang et al, 2017). The SEM investigations observed a good growth and attachment of G. thermodenitrificans Y7 on the surface of the switchgrass.…”

Section: Discussionsupporting

confidence: 75%

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Zhao

Meng

et al. 2020

Front. Microbiol.

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The composting ecosystem provides a potential resource for finding new microorganisms with the capability for cellulose degradation. In the present study, Congo red method was used for the isolating of thermostable lignocellulose-degrading bacteria from chicken manure compost. A thermophilic strain named as Geobacillus thermodenitrificans Y7 with acid-resident property was successfully isolated and employed to degrade raw switchgrass at 60 • C for 5 days, which resulted in the final degradation rates of cellulose, xylan, and acid-insoluble lignin as 18.64, 12.96, and 17.21%, respectively. In addition, GC-MS analysis about aromatic degradation affirm the degradation of lignin by G. thermodenitrificans Y7. Moreover, an endocellulase gene belong to M42 family was successfully cloned from G. thermodenitrificans Y7 and expressed in Escherichia coli BL21. Recombinant enzyme Cel-9 was purified by Ni-NTA column based the His-tag, and the molecular weight determined as 40.4 kDa by SDA-PAGE. The characterization of the enzyme Cel-9 indicated that the maximum enzyme activity was realized at 50 • C and pH 8.6 and, Mn 2+ could greatly improve the CMCase enzyme activity of Cel-9 at 10 mM, which was followed by Fe 2+ and Co 2+. Besides, it also found that the β-1,3-1,4, β-1,3, β-1,4, and β-1,6 glucan linkages all could be hydrolyzed by enzyme Cel-9. Finally, during the application of enzyme Cel-9 to switchgrass, the saccharification rates achieved to 1.81 ± 0.04% and 2.65 ± 0.03% for 50 and 100% crude enzyme, respectively. All these results indicated that both the strain G. thermodenitrificans Y7 and the recombinant endocellulase Cel-9 have the potential to be applied to the biomass industry.

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Section: Discussionsupporting

confidence: 75%

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Zhao

Meng

et al. 2020

Front. Microbiol.

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“…Thus, the hydrolysate produced using cellulase dosage of 5 FPU/g was studied further for cost effective fermentation. Kshirsagar et al (2016) reported 60% hydrolysis yield from enzymatic hydrolysis of wheat straw using crude enzyme from Amycolatopsis sp. GDS.…”

Section: Enzymatic Saccharification Of Sh and Bpshmentioning

confidence: 99%

Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production

et al. 2018

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HIGHLIGHTS Biological pretreatment of sorghum husk using Phanerochaete chrysosporium was investigated. Activities of different ligninolytic and hydrolytic enzymes during biological pretreatment were evaluated. Significant increase in reducing sugar production as a result of biological pretreatment of sorghum husk vs. untreated biomass. Biological pretreatment led to substantial surface morphological changes.

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“…Therefore, the complete genome of A. orientalis was sequenced, annotated and comparisons at the inter-and intra-generic level with different phylogenetic relatives within actinomycetes was done that led to the characterization of species specific and genus common features of the genome [3]. The genome of A. orientalis HCCB10007 comprises of two replicons, a large circular chromosome (8,948,591 bp) and a small dissociated circular plasmid pXL100 (33,499 bp). With the availability of complete genome sequences of other Amycolatopsis species, an intra-generic analysis was done using more rigorous statistical methods.…”

Section: Amycolatopsis Orientalis Hccb10007mentioning

confidence: 99%

“…Among these genera, the genus Amycolatopsis is of special importance for its capacity to produce several commercially and medicinally important antibiotics as balhimycin, vancomycin and rifamycin [2][3][4], other secondary metabolites as immuno-suppressants, anti-cancer agents etc. [5] and other applications [6][7][8]. These secondary metabolites often have diverse, unusual and complex structures and they are not essential for the growth of these organisms.…”

Section: Introductionmentioning

confidence: 99%

Genetics and Genomics of the Genus Amycolatopsis

2016

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Actinobacteria are gram-positive filamentous bacteria which contains some of the most deadly human pathogens (Mycobacterium tuberculosis, M. leprae, Corynebacterium diphtheriae, Nocardia farcinica), plant pathogens (Streptomyces scabies, Leifsonia xyli) along with organisms that produces antibiotic (Streptomycetes, Amycolatopsis, Salinospora). Interestingly, these bacteria are equipped with an extraordinary capability of producing antibiotics and other metabolites which have medicinal properties. With the advent of inexpensive genome sequencing techniques and their clinical importance, many genomes of Actinobacteria have been successfully sequenced. These days, with the constant increasing number of drug-resistant bacteria, the urgent need for discovering new antibiotics has emerged as a major scientific challenge. And, unfortunately the traditional method of screening bacterial strains for the production of antibiotics has decreased leading to a paradigm shift in the planning and execution of discovery of novel biosynthetic gene clusters via genome mining process. The entire focus has shifted to the evaluation of genetic capacity of organisms for metabolite production and activation of cryptic gene clusters. This has been made possible only due to the availability of genome sequencing and has been augmented by genomic studies and new biotechnological approaches. Through this article, we present the analysis of the genomes of species belonging to the genus Amycolatopsis, sequenced till date with a focus on completely sequenced genomes and their application for further studies.

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An isolatedAmycolatopsissp. GDS for cellulase and xylanase production using agricultural waste biomass

Abstract: This study demonstrates the importance of the isolate Amycolatopsis sp. GDS that secretes high levels of cellulase and hemicellulase by utilizing agricultural waste biomass and its application in the preparation of biomass feedstock and sequential ethanol fermentation.

Cited by 36 publications

References 42 publications

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Isolation of Thermostable Lignocellulosic Bacteria From Chicken Manure Compost and a M42 Family Endocellulase Cloning From Geobacillus thermodenitrificans Y7

Enzymatic hydrolysis of biologically pretreated sorghum husk for bioethanol production

Genetics and Genomics of the Genus Amycolatopsis

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