An isoform of ferritin as a component of protein yolk platelets in <i>Schistosoma mansoni</i>

Schüssler, P; Pötters, Elke; Winnen, Ralf; Bottke, Werner; Kunz, Werner

doi:10.1002/mrd.1080410307

Cited by 53 publications

(42 citation statements)

References 34 publications

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“…The discrepancy to about 800 bases, as measured for Ferl mRNA on northern blots (SchiiBler et al, 1995b), presumably is explained by the poly(A) tail. (Rothenberger et al, 1990).…”

Section: Resultsmentioning

confidence: 75%

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Ferritin mRNAs in Schistosoma Mansoni do not have Iron‐Responsive Elements for Post‐Transcriptional Regulation

Schüssler

Pötters

Winnen

et al. 1996

European Journal of Biochemistry

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Schistosomu mansoni possesses two isoforms of ferritin, soma and yolk ferritin. The soma ferritin occurs at a low level in most cells of both genders, whereas the yolk ferritin is a female-specific gene product that is expressed at high level in the vitellarium. In higher animals, ferritin mRNA is regulated by iron via the interaction of cytoplasmic binding proteins (IRPs) with a specific sequence element in the 5' untranslated region (UTR) referred to as the iron-responsive element (IRE). Sequence studies of the 5' UTRs, gel retardation assays, and hybridization experiments show that neither ferritin mRNAs of S. rnansoni is regulated by an IRE/IRP mechanism. It is suggested that ferritins in schistosomes are controlled only at the transcriptional level.

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“…The discrepancy to about 800 bases, as measured for Ferl mRNA on northern blots (SchiiBler et al, 1995b), presumably is explained by the poly(A) tail. (Rothenberger et al, 1990).…”

Section: Resultsmentioning

confidence: 75%

“…The discrepancy to about 950 bases, as measured for Fer2 mRNA on Northern blots (SchulJler et al, 1995b), presumably is explained by the poly(A) tail. The different lengths between the Ferl and Fer2 transcripts of 192 bases is reflected by different band sizes on northern blots (SchiiBler et al, 1995b).…”

Section: Resultsmentioning

confidence: 99%

Ferritin mRNAs in Schistosoma Mansoni do not have Iron‐Responsive Elements for Post‐Transcriptional Regulation

Schüssler

Pötters

Winnen

et al. 1996

European Journal of Biochemistry

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“…Wolbachia influences iron metabolism and ferritin is up-regulated when Wolbachia is absent, which may lead to apoptosis during oogenesis (86). Of interest also is that in Schistosoma mansoni one ferritin isoform is present at about 15-fold excess in females compared with males (87). The observation that ferritin like protein has higher expression for both occurrences in eggs from Male-biased females is thus of general interest given the endosymbiotic origin of mitochondria.…”

Section: Discussionmentioning

confidence: 99%

Proteomic Analysis of Eggs from Mytilus edulis Females Differing in Mitochondrial DNA Transmission Mode

Diz

Dudley

Cogswell

et al. 2013

Molecular & Cellular Proteomics

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Many bivalves have an unusual mechanism of mitochondrial DNA (mtDNA) inheritance called doubly uniparental inheritance (DUI) in which distinctly different genomes are inherited through the female (F genome) and male (M genome) lineages. In fertilized eggs that will develop into male embryos, the sperm mitochondria remain in an aggregation, which is believed to be delivered to the primordial germ cells and passed to the next generation through the sperm. In fertilized eggs that will develop into female embryos, the sperm mitochondria are dispersed throughout the developing embryo and make little if any contribution to the next generation. The frequency of embryos with the aggregated or dispersed mitochondrial type varies among females. Previous models of DUI have predicted that maternal nuclear factors cause molecular differences among unfertilized eggs from females producing embryos with predominantly dispersed or aggregated mitochondria. We test this hypothesis using females of each of the two types from a natural population. We have found small, yet detectable, differences of the predicted type at the proteome level. We also provide evidence that eggs of females giving the dispersed pattern have consistently lower expression for different proteasome subunits than eggs of females giving the aggregated pattern. These results, combined with those of an earlier study in which we used hatchery lines of Mytilus, and with a transcriptomic study in a clam that has the DUI system of mtDNA transmission, reinforce the hypothesis that the ubiquitinproteasome system plays a key role in the mechanism of DUI and sex determination in bivalves. We also report that eggs of females giving the dispersed pattern have higher expression for arginine kinase and enolase, enzymes involved in energy production, whereas ferritin, which is involved in iron homeostasis, has lower expression. We discuss these results in the context of genetic models for DUI and suggest experimental methods for further understanding the role of these proteins in DUI. Proteomics has made a rapid progress when applied to model species where genomic databases are well developed, but recent reviews have pointed to the growing interest in applying proteomics to nonmodel species in areas such as evolutionary ecology (1), aquatic toxicology (2), aquatic pollution (3), aquaculture (4), and marine biology (5). In the marine mussel genus Mytilus, an unusual system of mtDNA inheritance occurs characterized by the presence of two mitochondrial genomes (called F and M) one of which is inherited maternally and the other paternally (6 -9). This phenomenon, called doubly uniparental inheritance of mtDNA (DUI), 1 suggests a connection between mtDNA inheritance and sex determination. DUI also occurs in other species belonging to three bivalve orders (10, 11). Since the 1990s, DUI has been under intensive investigation, but the molecular mechanism for DUI and how it relates to sex determination, is still to be elucidated (for review, see 12-15).Cytological studies of fertilize...

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“…For genes expressed in the reproductive organs of paired females such as the eggshell precursor protein genes p14 (Koster et al 1988) and p19 (Michel et al 2003), the iron storage protein gene ferritin I (Schussler et al 1995), and a mucin-like protein gene (Menrath et al 1995) an influence of the male on transcription was documented. After separation from the male partner, the transcriptional activity of these genes declined, but was restored after re-pairing (Chen et al 1992;Grevelding et al 1997;Michel et al 2003).…”

Section: Biology Of Male-female Interactionmentioning

confidence: 99%

Sex in Schistosomes – Signaling Mechanisms in the Female Gonads

Beckmann

Quack

Burmeister

et al. 2011

Progress in Parasitology

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Besides their great medical importance as causative agents of schistosomiasis, an infectious disease affecting humans and animals in tropical and subtropical regions worldwide, schistosomes exhibit distinctive biological features. Living in the blood vessels of infected hosts, these blood flukes survive permanent attacks of the immune system over many years. Furthermore, schistosomes represent the only genus of the class trematoda which live doeciously. Their most remarkable attribute, however, is the continuous pairing-contact which is both obligatory for the development of the female reproductive organs and prerequisite for egg production Over decades great efforts have been undertaken to develop an effective vaccine against schistosomes, but without fundamental success. In addition, due to the upcoming fear for resistance against the commonly used drug praziquantel, there is a pressing need to find new drugs to fight this parasite. In this respect, the understanding of basic processes of schistosome biology, especially its reproductive development, is fundamental. Since egg production is closely associated with the clinical progression of schistosomiasis, elucidating the molecular principles of the male-induced sexual maturation of the female may lead to new strategies intervening in these processes to control parasite spread on the one hand and to limit egg-induced pathology on the other.

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An isoform of ferritin as a component of protein yolk platelets in Schistosoma mansoni

Cited by 53 publications

References 34 publications

Ferritin mRNAs in Schistosoma Mansoni do not have Iron‐Responsive Elements for Post‐Transcriptional Regulation

Ferritin mRNAs in Schistosoma Mansoni do not have Iron‐Responsive Elements for Post‐Transcriptional Regulation

Proteomic Analysis of Eggs from Mytilus edulis Females Differing in Mitochondrial DNA Transmission Mode

Sex in Schistosomes – Signaling Mechanisms in the Female Gonads

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