An Isocratic HPLC Method for the Determination of Sorbitol and Glycerol in Pharmaceutical Formulations

Abstract

Sorbitol and glycerol, along with other inactive ingredients such as preservatives and dyes, are commonly used in various pharmaceutical and personal care products. To accurately assess the effectiveness of various formulations containing sorbitol and/or glycerol, their quantitative determination is essential. In the current study, two types of detectors (a Varian evaporative light scattering detector and an Agilent ultraviolet-visible detector) are evaluated for the assay of working sample solutions. The two detection techniques are complimentary, and a comparison of the results obtained using the two detectors is presented here. The current method is shown to be stability-indicating and free from interference from any of the formulation excipients and potential degradation products. The method is reproducible, accurate, sensitive and selective. It provides enhanced detection sensitivity for sorbitol and comparable sensitivity for glycerol versus similar methods reported in the literature that utilize a refractive index detector for the analysis of either of the two polyols.

Synthesis of Bio-hexane and Bio-Hexene from Sorbitol Using Formic Acid as Reducing Agent

“…These peaks confirm the presence of alkenes with terminal and non-terminal double bonds as well as alkanes. These results are basically consistent with the results reported by other researchers [10][11][12][13]. The FTIR spectrum of the bio-hydrocarbons produced by deiodizing 2-iodohexane by gas phase pyrolysis at 260 °C for 45 minutes are shown in Figure 8.…”

“…Sulfuric acid (H2SO4) solution 0.005 N was used as the mobile phase. The flow rate of the mobile phase was maintained at 0.6 mL/min by the column temperature at 65 °C [13]. A relative index detector was attached to measure what amount of each compound passed the detector during a certain retention time.…”

Hydroxyapatite delivery to dentine tubules using carboxymethyl cellulose dental hydrogel for treatment of dentine hypersensitivity

“…The ingredients of the hydrogel were adjusted to have the viscosity and feel of toothpaste. CMC, numerous hydroxyl and carboxylic groups and sorbitol and glycerol, which are all known humectants, all have the ability to retain water and so affect viscosity [29,30]. Presently, the concentrations of these materials were fixed and that it was observed that the increase in HAp concentration progressively increased caused the viscosity of the fabricated hydrogels.…”

Study on detection methods for sorbitol

“…In recent years, HPLC has been combined with many sensitive detection techniques and has experienced continuous improvement of stationary phases, which have improved its sensitivity and specificity. HPLC is currently widely used for the analysis of drugs and dosage forms with respect to quality control, quantitative determination of active ingredients and impurities, monitoring drug blood concentration in patients, and bioequivalence assessment [10][11][12].…”