An ionic liquid supported CeO2 nanoshuttles–carbon nanotubes composite as a platform for impedance DNA hybridization sensing

Zhang, Wei; Yang, Tao; Zhuang, Xuming; Guo, Zhiyan; Jiao, Kui

doi:10.1016/j.bios.2008.12.024

Cited by 118 publications

(46 citation statements)

References 35 publications

(36 reference statements)

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“…27 Zhang et al used 1-butyl-3-methylimidazolium hexafluorophosphate (BMIMPF 6 ) supported CeO2 nanoshuttles-carbon nanotubes composite as a platform for impedance DNA hybridization sensing in hope of enhancing the sensitivity of the biosensor. 28 Despite some advantages of BMIMPF6, it can't be well dissolved in DMF. The hydrophobicity confined the joint use of BMIMPF6 and some water-solubility materials.…”

Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for Myeloperoxidase in Human Serum Based on a Multi-wall Carbon Nanotubes-Ionic Liquid-Cerium Dioxide Film-modified Electrode

Lu¹,

Liu²,

Liu³

2010

Bulletin of the Korean Chemical Society

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A label-free amperometric immunosensor has been proposed for the detection of myeloperoxidase (MPO) in human serum. To fabricate such an immunosensor, a composite film consisting of N,N-dimethylformamide (DMF), multiwall carbon nanotubes (MWCNTs) and 1-ethyl-3-methyl imidazolium tetrafluoroborate (EMIMBF4) suspension was initially formed on a glassy carbon electrode (GCE). Then cerium dioxide (CeO2) dispersed by chitosan was coated on the GCE. After that, MPO antibodies (anti-MPO) were attached onto the nanoCeO2 surface. With a noncompetitive immunoassay format, the antibody-antigen complex formed between the immobilized anti-MPO and MPO in sample solution. The immunosensor was characterized by cyclic voltammetry, transmission electron microscopy (TEM) and scanning electron microscopy (SEM). The factors influencing the performance of the immunosensor were studied in detail. Under optimal conditions, the current change before and after the immunoreaction was proportional to MPO concentration in the range of 5 to 300 ng mL -1 with a detection limit of 0.2 ng mL -1.

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Section: Introductionmentioning

confidence: 99%

Amperometric Immunosensor for Myeloperoxidase in Human Serum Based on a Multi-wall Carbon Nanotubes-Ionic Liquid-Cerium Dioxide Film-modified Electrode

Lu¹,

Liu²,

Liu³

2010

Bulletin of the Korean Chemical Society

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“…EIS is very sensitive to changes in interfacial impedance upon biorecognition events occurring at the surface-electrolyte interface. [40,41] The EIS measurements were carried out in PBS (pH 7.0) in the frequency range from 1 to 10 5 Hz, and the Bode plots are shown in Figure 5 B. Curve a is the Bode plot of PABSA/TiO 2 /CPE. When the pDNA was covalently bound onto PABSA/TiO 2 nanosheets, the impedance increased (curve b).…”

Section: Selection Of Pulse Timementioning

confidence: 99%

Highly Sensitive Indicator‐Free Impedance Sensing of DNA Hybridization Based on Poly(m‐aminobenzenesulfonic acid)/TiO₂ Nanosheet Membranes with Pulse Potentiostatic Method Preparation

Yang

Wang

et al. 2010

Chemistry A European J

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A direct electrochemical detection procedure for DNA hybridization by using the electrochemical signal changes of conductive poly(m-aminobenzenesulfonic) acid (PABSA)/TiO(2) nanosheet membranes, which were electropolymerized by using the pulse potentiostatic method, is reported. Due to the unique properties of TiO(2) nanoparticles, m-aminobenzenesulfonic acid monomers tend to be adsorbed around the particles, and the electropolymerization efficiency is greatly improved. The combination of TiO(2) nanoparticles and PABSA resulted in a nanocomposite membrane with unique and novel nanosheet morphology that provides more activation sites and enhances the surface electron-transfer rate. These characteristics were propitious for the magnification of PABSA electrochemical signals and the direct detection of DNA hybridization. Owing to the presence of abundant sulfonic acid groups, PABSA could overcome the drawbacks of polyaniline and be used to detect bioanalytes at physiological pH. DNA probes could be covalently attached to the sulfonic groups through the amines of DNA sequences by using an acyl chloride cross-linking reaction. After immobilization of probe DNA, the electrochemical impedance value increased significantly compared to that of PABSA/TiO(2) nanosheet membranes, and then decreased dramatically after the hybridization reaction of the probe DNA with the complementary DNA sequence compared to that of the probe-immobilized electrode. Electrochemical impedance spectroscopy was adopted for indicator-free DNA biosensing, which had an eminent ability for the recognition between double-base mismatched sequences or non-complementary DNA sequences and complementary DNA sequences. A gene fragment, which is related to one of the screening genes for the transgenically modified plants, the cauliflower mosaic virus 35S gene was satisfactorily detected. This is the first report for the indicator-free impedance DNA hybridization detection by using PABSA/TiO(2) membranes under neutral conditions.

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“…There are two aspects about effects of ILs on these supermolecules, which influence the system individually or jointly. One is that ILs affect the supermolecules because of the anions and cations of ILs and aromatic structures, which can result in electrostatic interactions, cation-π and/or π-π stacking interactions (Gopalan et al, 2009;Park et al, 2009a;Park et al, 2009b;Wu et al, 2009a;Zhang et al, 2009). The other is that self-assemby of ILs influence the supermolecules (Gutel et al, 2007;Ma et al, 2009;).…”

Section: Effects Of Ionic Liquids On Other Supermoleculesmentioning

confidence: 99%

Supramolecular Structures in the Presence of Ionic Liquids

Shen¹,

Chen²,

Zhang³

et al. 2011

Ionic Liquids: Theory, Properties, New Approaches

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An ionic liquid supported CeO2 nanoshuttles–carbon nanotubes composite as a platform for impedance DNA hybridization sensing

Cited by 118 publications

References 35 publications

Amperometric Immunosensor for Myeloperoxidase in Human Serum Based on a Multi-wall Carbon Nanotubes-Ionic Liquid-Cerium Dioxide Film-modified Electrode

Amperometric Immunosensor for Myeloperoxidase in Human Serum Based on a Multi-wall Carbon Nanotubes-Ionic Liquid-Cerium Dioxide Film-modified Electrode

Highly Sensitive Indicator‐Free Impedance Sensing of DNA Hybridization Based on Poly(m‐aminobenzenesulfonic acid)/TiO₂ Nanosheet Membranes with Pulse Potentiostatic Method Preparation

Supramolecular Structures in the Presence of Ionic Liquids

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An ionic liquid supported CeO2 nanoshuttles–carbon nanotubes composite as a platform for impedance DNA hybridization sensing

Cited by 118 publications

References 35 publications

Amperometric Immunosensor for Myeloperoxidase in Human Serum Based on a Multi-wall Carbon Nanotubes-Ionic Liquid-Cerium Dioxide Film-modified Electrode

Amperometric Immunosensor for Myeloperoxidase in Human Serum Based on a Multi-wall Carbon Nanotubes-Ionic Liquid-Cerium Dioxide Film-modified Electrode

Highly Sensitive Indicator‐Free Impedance Sensing of DNA Hybridization Based on Poly(m‐aminobenzenesulfonic acid)/TiO2 Nanosheet Membranes with Pulse Potentiostatic Method Preparation

Supramolecular Structures in the Presence of Ionic Liquids

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Product

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Highly Sensitive Indicator‐Free Impedance Sensing of DNA Hybridization Based on Poly(m‐aminobenzenesulfonic acid)/TiO₂ Nanosheet Membranes with Pulse Potentiostatic Method Preparation