An investigation on the sperm number and reproductive parameters of males in wild caught freshwater crayfish (Astacus leptodactylus, Eschscholtz)

Harlıoğlu, Muzaffer Mustafa; Kutluyer, Filiz; Gür, Seyfettin

doi:10.1163/157075612x638559

Cited by 24 publications

(21 citation statements)

References 26 publications

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“…The number of sperm cells observed for several decapod species is a proxy for sperm quality (Bugnot & López‐Greco, ; Claudet et al, ; Coman et al, ; Harlıoğlu, Kutluyer, & Gür, ). M. rosenbergii, with an average weight of 16 g produces 2.5 × 10 6 sperm cells per spermatophore (Revathi, Iyapparaj, Vasanthi, Munuswamy, & Krishnan, ); 5.2–5.8 × 10 8 sperm cells for males of 24 ± 10 g weight (Harris & Sandifer, ); and 6 ± 4 × 10 9 for males with an average body weight of 45 ± 4 g (Claudet et al, ).…”

Section: Discussionmentioning

confidence: 99%

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Spermatophore production and sperm quality of the river prawn Macrobrachium americanum Spence Bate, 1868 fed with different diets

et al. 2019

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The effects of different diets on spermatophore production and sperm quality were investigated in the river prawn Macrobrachium americanum. River prawns were cultured and fed with three diets for 244 days: fresh food (50% squid meat, Dosidicus gigas and 50% sardine muscle, Sardinops sagax); commercial pellets (35 Purina®); and a 50:50 mixture of both diets. Spermatophore production was recorded every 24 days on average as the percentage of spermatophores produced per extraction per diet, weight and biochemical composition. Sperm quality was measured as the total number of sperm, the proportion of live/dead sperm and normal/abnormal sperm morphology. There were no significant differences in the mean biochemical composition of M. americanum spermatophores for any of the diets. Biochemical composition was 36.3% protein, 25.8% carbohydrate and 4.6% lipids for all data pooled. The weight of spermatophores and sperm counts was not significantly different among diets, nor were there any differences as a function of the male initial total length (p > .05). Male river prawn reproductive exhaustion was observed as a decline in spermatophore production, weight of the spermatophores and the number of sperm cells per spermatophore, with an increasing proportion of dead and abnormal sperm seen throughout the experiment. The recommended period of maintenance in captivity for male broodstock is less than 115 days. It is recommended to feed broodstock males of M. americanum with commercial pellets because no significant differences were detected with the diets tested; pellets are easier to use, ensuring the same spermatophore production and sperm quality that was obtained with fresh food.

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Section: Discussionmentioning

confidence: 99%

“…The number of sperm cells observed for several decapod species is a proxy for sperm quality (Bugnot & López-Greco, 2009;Claudet et al, 2016;Coman et al, 2007;Harlıoğlu, Kutluyer, & Gür, 2012).…”

Section: Number Of Sperm Cells Per Spermatophorementioning

confidence: 99%

Spermatophore production and sperm quality of the river prawn Macrobrachium americanum Spence Bate, 1868 fed with different diets

et al. 2019

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“…Our study aimed to develop a reliable protocol to estimate male fertility through sperm counts of single Daphnia male individuals using flow cytometry. Considering the known relationship between sperm count and organism size in crustaceans (Bugnot & López Greco, ; Ceballos‐Vázquez, Rosas, & Racotta, ; Harlioğlu, Kutluyer, & Gür, ; Peixoto et al, ; Sato, Yoseda, Abe, & Shibuno, ), we report here sperm number as a function of male size. We provide the first comparative data of sperm counts from multiple clones of three species of Daphnia ( D. pulex , D. pulicaria , and D. magna ).…”

Section: Introductionmentioning

confidence: 90%

Sperm number assessed by flow cytometry in species of Daphnia (Crustacea, Cladocera)

Belzile

McKindsey

Beaudreau

2019

Invertebrate Biology

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Daphnia magna and Daphnia pulex are two important model species in ecotoxicology.In daphniids, studies of the effects of contaminants have mostly focused on female life history traits, yet it would also be important to examine male reproductive traits, particularly in relation to endocrine disruptors. In this study, we developed a protocol that uses flow cytometry to measure sperm number in individual males of different species of Daphnia. We tested our protocol on 114 males from several clones of three common species of Daphnia. Sperm count varied widely among individuals and reached high numbers (up to 1.45 × 10 5 ). Positive relationships between male length and sperm number were observed in D. pulex and Daphnia pulicaria, but not in D. magna. Important inter-clonal differences in sperm production were observed in all species, with some clones producing very little sperm. Duplicated sperm samples showed on average only 6% difference in sperm counts. Sperm counts were stable at least over a 2-hr period and up to 5 hr for most samples. This sperm isolation protocol and flow cytometric enumeration approach will be of major interest to ecotoxicologists. K E Y W O R D SDaphnia magna, Daphnia pulex, ecotoxicology, invertebrates, sperm quality

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“…At the end of the experiment, the GSI, VDI, TI, HSI and ovarian egg number was determined according to Harlıoğlu, Kutluyer, and Gür ():

GSI false(% false) = (\frac{Reproductive system wet weight}{Body wet weight}) \times 100

VDI false(% false) = (\frac{Vas deferens wet weight}{Body wet weight}) \times 100

TI false(% false) = (\frac{Testes wet weight}{Body wet weight}) \times 100

HSI false(% false) = (\frac{Hepatopancreas wet weight}{Body wet weight}) \times 100

…”

Section: Methodsmentioning

confidence: 99%

Effect of serotonin injection on the reproductive parameters and haemolymph methyl farnesoate level in the narrow‐clawed crayfishPontastacus leptodactylus(Eschscholtz, 1823)

2019

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The effects of serotonin injection (1, 5 and 10 µg/g body weight) on spermatozoal production, vas deferens index (VDI), hepatosomatic index (HSI), testicular index (TI), gonado‐somatic index (GSI), ovarian egg number, total protein, methyl farnesoate (MF) levels of haemolymph, amino acid and fatty acid profiles of hepatopancreas were evaluated in Pontastacus leptodactylus. In male P. leptodactylus, the highest spermatozoal number (9.3 × 106 ± 1.2) and MF level (0.66 ± 0.06 ng−1 ml) were observed after injection of 10 µg/g of serotonin (p < .05). Serotonin injection, however, did not significantly affect the reproductive parameters in female P. leptodactylus (p > .05). It was also found that serotonin injection caused an increase in the level of MF in male P. leptodactylus but a reduction in female P. leptodactylus (p < .05). Therefore, a sex‐dependent response to serotonin could exist for MF synthesis in P. leptodactylus. Serotonin injection did not affect the total protein, amino acid and fatty acid profiles in the hepatopancreas of male P. leptodactylus (p > .05). It can be concluded that serotonin injection affects the reproductive parameters such as spermatozoal production, VDI, TI, GSI, HSI and haemolymph MF levels in P. leptodactylus.

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An investigation on the sperm number and reproductive parameters of males in wild caught freshwater crayfish (Astacus leptodactylus, Eschscholtz)

Cited by 24 publications

References 26 publications

Spermatophore production and sperm quality of the river prawn Macrobrachium americanum Spence Bate, 1868 fed with different diets

Spermatophore production and sperm quality of the river prawn Macrobrachium americanum Spence Bate, 1868 fed with different diets

Sperm number assessed by flow cytometry in species of Daphnia (Crustacea, Cladocera)

Effect of serotonin injection on the reproductive parameters and haemolymph methyl farnesoate level in the narrow‐clawed crayfishPontastacus leptodactylus(Eschscholtz, 1823)

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