An investigation on allele frequency at the &lt;i&gt;CSN1S2&lt;/i&gt; locus and its relationship with milk parameters in the Sarda goat

Vacca, Giuseppe Massimo; Ali, Haider; Pazzola, Michele; Sanna, Maria Lina; Dettori, Maria Luisa; Carcangiu, Vincenzo

doi:10.22358/jafs/66437/2009

Cited by 8 publications

(10 citation statements)

References 23 publications

(27 reference statements)

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“…In this study, A, B, C, and F alleles were observed in the South African dairy goat populations, with the A allele occurring at the highest frequency, followed by the F allele. Similar frequencies for allele A and F were reported in Italian (Vacca et al, 2009), Sicilian (Gigli et al, 2008;Palmeri et al, 2014), and West African goat breeds (Caroli et al, 2006;Caroli et al, 2007). The high occurrence of allele A in the South African goat population may be attributed to the A allele being considered almost fixed in the majority of Western breeds (Selvaggi & Tufarelli, 2011).…”

Section: Discussionsupporting

confidence: 63%

“…The Toggenburg population had a frequency of 0.225 for CSN1S2 B, which was higher than the low frequencies of between 0.005 and 0.051 reported by Caroli et al (2006;, Gigli et al (2008) and Vacca et al (2009;2014). Furthermore, the AB genotype was observed in all the populations except for the Saanen population.…”

Section: Discussionmentioning

confidence: 58%

“…Although six of the ten genotypes observed in the total population were heterozygous, most of them occurred in the Toggenburg population with very low frequencies (0.050). These genotypes contain rare alleles in which the effect on milk production traits is largely unknown and requires further investigation, especially since these genotypes were not reported in any other goat breeds that have been studied (Palmeri et al, 2014;Vacca et al, 2009;2014).…”

Section: Discussionmentioning

confidence: 99%

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Genetic polymorphism of CSN1S2 in South African dairy goat populations

Grobler¹,

Visser²,

Chessa³

et al. 2017

SA J. An. Sci.

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Alpha-s 2 casein has a significant influence on protein content in goat milk, and the technological properties important for cheese processing. Specific alleles (A, B, C, E and F) of the alpha (α) s2 -casein gene (CSN1S2) result in higher protein, casein and fat content, and improved coagulation properties, which are useful for improved cheese making. The aim of this study was to investigate the polymorphism and genetic variation of CSN1S2 in South African dairy goats, using DNA sequencing technology. Sixty dairy goats (20 Saanes, 20 British Alpine, and 20 Toggenburg) and 20 meat-type goats were sequenced with four primers to distinguish among the seven known alleles for α s2 -casein. A total of four alleles (A, B, C and F) for CSN1S2 were observed among the dairy-and meat-type populations with ten genotypes across the populations. The A allele and the AA genotype were the most frequent across the populations, with the favourable AC genotype being the most frequent (0.300) in the Saanen population. Two unique genotypes were detected in the Toggenburg (BB and BF) and one in the meat-type goats (CF). The results indicate moderate genetic variation for α s2 -casein in the South African goat populations (42.3-63.6%). Low positive F ST values suggest limited inbreeding. This study confirmed the presence of favourable alleles in the South African goat populations, indicating room for genetic improvement using directional selection for favourable genotypes.

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Section: Discussionsupporting

confidence: 63%

Section: Discussionmentioning

confidence: 58%

Section: Discussionmentioning

confidence: 99%

See 1 more Smart Citation

Genetic polymorphism of CSN1S2 in South African dairy goat populations

Grobler¹,

Visser²,

Chessa³

et al. 2017

SA J. An. Sci.

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“…The CSN1S2 B and C alleles were characterized by Allele Specific-PCR (Vacca et al, 2009b). Since primer pair used in Allele Specific-PCR did not discriminate C and E alleles, C allele was assigned after CSN1S2 E allele identification which was obtained using primer pair by Chessa et al (2008) and restriction enzyme by Lagonigro et al (2001).…”

Section: Methodsmentioning

confidence: 99%

Genetic Variability atαs₂-caseinGene inGirgentanaDairy Goat Breed

Palmeri

Mastrangelo

Sardina

et al. 2014

Italian Journal of Animal Science

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Casein genes are highly polymorphic and the high degree of variability has qualitative and quantitative effects on milk composition thereby affecting chemical, physical and technological properties of goat milk. The aim of this work was to evaluate the genetic polymorphisms of the as2-casein (CSN1S2) gene in the endangered Girgentana dairy goat breed in order to assess the genotypes distribution, as it is known genotype influences technological and nutritional milk properties. The study was performed on 207 sample of Girgentana goat breed, analysed with different PCR protocols. The most frequent alleles was A (0.722), followed by F (0.225), C (0.051) and E (0.002) while B, D and 0 alleles were not found. Genotypes detected were AA (0.512), AF (0.338), AC (0.082), FF (0.043), CF (0.020) and EF (0.005). Our results suggested that Girgentana goat breed could be used for the production of milk with high fat and protein content and with optimal technological ability, suitable for cheese making.

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“…At CSN1S2 locus alleles B and C/E were detected by multiplex AS-PCR (Vacca et al, 2009). The allele E was identified by PCR-RFLP protocol of Lagonigro et al (2001) using primers by Chessa, Rignanese, Chiatti, et al (2008).…”

Section: Reagents Standards and Samplesmentioning

confidence: 99%