An Investigation of Two Kinds of Magnetic Chitosan Composite Microspheres

Zhang, Shengtang; Wu, None Guangpeng; Wang, Wenqiang; Gao, Sufang; Zhang, Ji; Wang, Yun‐Pu

doi:10.1177/0883911507079967

Cited by 3 publications

(1 citation statement)

References 22 publications

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“…Lactose was purchased from the local market. The magnetic beads were the products of our laboratory [12,13]. Glutaraldehyde 50% (w/v) used as a cross-linking reagent as well as other chemicals was of analytical grade.…”

Section: Methodsmentioning

confidence: 99%

Immobilization of β-Galactosidase onto Magnetic Beads

Zhang¹,

Gao

Gao³

2009

Appl Biochem Biotechnol

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A study of the cross-linking of beta-galactosidase on magnetic beads is reported here. The magnetic beads were prepared from artemisia seed gum, chitosan, and magnetic fluid in the presence of a cross-linking regent (i.e., glutaraldehyde). The reactive aldehyde groups of the magnetic beads allowed the reaction of the amino groups of the enzymes. The animated magnetic beads were used for the covalent immobilization of beta-galactosidase. The effect of various preparation conditions on the activity of the immobilized beta-galactosidase, such as immobilizing time, amount of enzyme, and the concentration of glutaraldehyde, were investigated. The influence of pH and temperature on the activity and the stability of the enzyme, both free and immobilized, have been studied. And o-nitrophenyl-beta-D: -galactopyranoside (ONPG) was chosen as a substrate. The beta-galactosidase immobilized on the magnetic beads resulted in an increase in enzyme stability. Optimum operational temperature for immobilized enzyme was 10 degrees Celsius higher than that of free enzyme and was significantly broader.

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Section: Methodsmentioning

confidence: 99%

Immobilization of β-Galactosidase onto Magnetic Beads

Zhang¹,

Gao

Gao³

2009

Appl Biochem Biotechnol

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Chitosan core-corona nanospheres: A convenient material to tailor pH and solvent responsive magnetic nanoparticles

Chatrabhuti

Chirachanchai

2013

Polymer

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Microwave-assisted Preparation of Magnetic Albumin Microspheres

Chen

et al. 2008

Journal of Bioactive and Compatible Polymers

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A new microwave-assisted method was used to prepare magnetic Fe3 O4 particles and magnetic bovine albumin microspheres. The microwave method produced smaller particles and is faster than traditional methods. The optimum conditions to prepare the Fe3O4 particles were three minutes at pH 13 and 80°C. Magnetic microspheres containing albumin were synthesized based on heating times and temperatures to form microspheres with different properties. For example, heating for 4min, at 160°C, yielded smaller sized microspheres (30 μm). Confirmed by XRD, SEM, and FT-IR that iron oxide particles were encapsulated in biocompatible proteins, The thermal stability of the microspheres were determined by DSC and TG. The magnetic properties were determined by UV—VIS spectraphotometry and a Guoy magnetic balance. This microwave process could become a preferred method for the synthesis of magnetized protein microspheres.

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An Investigation of Two Kinds of Magnetic Chitosan Composite Microspheres

Cited by 3 publications

References 22 publications

Immobilization of β-Galactosidase onto Magnetic Beads

Immobilization of β-Galactosidase onto Magnetic Beads

Chitosan core-corona nanospheres: A convenient material to tailor pH and solvent responsive magnetic nanoparticles

Microwave-assisted Preparation of Magnetic Albumin Microspheres

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