An investigation of the effects of TETRA RF fields on intracellular calcium in neurones and cardiac myocytes

Green, A C; Scott, I.R.; Gwyther, Robert J; Peyman, Azadeh; Chadwick, P. M. E.; Chen, X; Alfadhl, Yasir; Tattersall, J.E.H.

doi:10.1080/09553000600555389

Cited by 14 publications

(12 citation statements)

References 39 publications

(48 reference statements)

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“…Schwartz et al [1990] reported an increased calcium efflux in whole frog hearts. Other studies, however, have not reported altered calcium physiology in neurons or cardiac tissue due to EMF [Schwartz and Mealing, 1993;Wolke et al, 1996;Green et al, 2005]. Some studies have focused on the direct effects on electrophysiological properties during the course of exposure [Caddemi et al, 1986].…”

Section: Discussionmentioning

confidence: 94%

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

Daus

Goldhammer

Layer

et al. 2011

Bioelectromagnetics

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In recent years, a number of in vitro studies have reported on the possible athermal effects of electromagnetic exposure on biological tissue. Typically, this kind of study is performed on monolayers of primary cells or cell lines. However, two-dimensional cell layer systems lack physiological relevance since cells in vivo are organized in a three-dimensional (3D) architecture. In monolayer studies, cell-cell and cell-ECM interactions obviously differ from live tissue and scale-ups of experimental results to in vivo systems should be considered carefully. To overcome this problem, we used a scaffold-free 3D cell culture system, suitable for the exploration of electrophysiological effects due to electromagnetic fields (EMF) at 900 MHz. Dissociated cardiac myocytes were reaggregated into cellular spheres by constant rotation, and non-invasive extracellular recordings of these so-called spheroids were performed with microelectrode arrays (MEA). In this study, 3D cell culture systems were exposed to pulsed EMFs in a stripline setup. We found that inhomogeneities in the EMF due to electrodes and conducting lines of the MEA chip had only a minor influence on the field distribution in the spheroid if the exposure parameters were chosen carefully.

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Section: Discussionmentioning

confidence: 94%

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

Daus

Goldhammer

Layer

et al. 2011

Bioelectromagnetics

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“…Using a SAR of 2 W/kg and a GSM signal (915 MHz), Cranfield et al [2001] found that calcium concentration in Jurkat cells did not change during 10 min of exposure. In a recent study, Green et al [2005] did not find any effect on [Ca 2þ ] i in rat cerebellar neurons and cardiac myocytes, using a TETRA signal (0.4 W/kg) and a longer time (20 min) of exposure. A very recent study carried out in ratcultured hippocampal neurons has shown that chronic exposure (15 min/day for 8 days) to GSM signal (1800 MHz, 2.4 W/kg) decreased both amplitude of aamino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA) miniature excitatory post-synaptic currents, and the expression of post-synaptic density 95 [Xu et al, 2006].…”

Section: Discussionmentioning

confidence: 94%

“…More recent studies using direct measurements of [Ca 2þ ] i on different cells did not show significant changes following RF exposure. In particular, no effects were observed in guinea pig ventricular myocytes [Wolke et al, 1996], Jurkat leukemic T-cells [Cranfield et al, 2001], rat cerebellar granule cells, and cardiac myocytes [Green et al, 2005].…”

Section: Introductionmentioning

confidence: 96%

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

Platano

Mesirca

Paffi

et al. 2007

Bioelectromagnetics

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We have studied the non-thermal effects of radiofrequency (RF) electromagnetic fields (EMFs) on Ba(2+) currents (I Ba 2+) through voltage-gated calcium channels (VGCC), recorded in primary cultures of rat cortical neurons using the patch-clamp technique. To assess whether low-level acute RF field exposure could modify the amplitude and/or the voltage-dependence of I Ba 2+, Petri dishes containing cultured neurons were exposed for 1-3 periods of 90 s to 900 MHz RF-EMF continuous wave (CW) or amplitude-modulated according to global system mobile communication standard (GSM) during whole-cell recording. The specific absorption rates (SARs) were 2 W/kg for CW and 2 W/kg (time average value) for GSM-modulated signals, respectively. The results obtained indicate that single or multiple acute exposures to either CW or GSM-modulated 900 MHz RF-EMFs do not significantly alter the current amplitude or the current-voltage relationship of I Ba 2+, through VGCC.

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“…which are also eigenfunctions of the Hamiltonian, degenerated with (5). Once more, we refer to [1] for further discussion.…”

Section: Formalism and Plasmamentioning

confidence: 99%

“…There is, of course, a vast amount of literature on biological effects of electromagnetic radiation. Some more recent examples are [3][4][5][6][7]. It is not the purpose of this study to discuss these papers; a recent review, including many references, is given in [8].…”

Section: Introductionmentioning

confidence: 99%

Ion Plasma Responses to External Electromagnetic Fields

Naus

2010

SRX Physics

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The response of ion plasmas to external radiation fields is investigated in a quantum mechanical formalism. We focus on the total electric field within the plasma. For general bandpass signals three frequency regions can be distinguished in terms of the plasma frequency. For low frequencies, the external field is shielded. For high frequencies, the field is not modified. Resonant behavior of the plasma appears for frequencies near the plasma frequency: large internal electric fields and induced currents are present. These effects may be relevant for biological systems. The model is therefore extended to a two-species plasma and additional interactions are studied. The response is not essentially altered. To make the models more realistic, a so-called bath is included. In the weak coupling approximation the resonance frequency is shifted and some damping occurs. Finite temperature effects on the electric field are absent. The energy of the system, however, depends on temperature.

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An investigation of the effects of TETRA RF fields on intracellular calcium in neurones and cardiac myocytes

Abstract: Overall, these results showed no evidence of any consistent or biologically relevant effect of TETRA fields on [Ca(2 + )](i) in granule cells and cardiac myocytes at any of the SAR tested.

Cited by 14 publications

References 39 publications

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons

Ion Plasma Responses to External Electromagnetic Fields

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An investigation of the effects of TETRA RF fields on intracellular calcium in neurones and cardiac myocytes

Abstract: Overall, these results showed no evidence of any consistent or biologically relevant effect of TETRA fields on [Ca(2 + )](i) in granule cells and cardiac myocytes at any of the SAR tested.

Cited by 14 publications

References 39 publications

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

Electromagnetic exposure of scaffold-free three-dimensional cell culture systems

Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba2+ currents through voltage‐gated calcium channels in rat cortical neurons

Ion Plasma Responses to External Electromagnetic Fields

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Acute exposure to low‐level CW and GSM‐modulated 900 MHz radiofrequency does not affect Ba²⁺ currents through voltage‐gated calcium channels in rat cortical neurons