An Investigation of Lymphocyte Production in Guinea-Pig Bone Marrow

Harris, Paul J.; Kugler, J. H.

doi:10.1159/000209547

Cited by 18 publications

(3 citation statements)

References 11 publications

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“…These observations corroborate the findings of other reports (11,12,13) that lymphocytes in bone marrow synthesize DNA. These observations corroborate the findings of other reports (11,12,13) that lymphocytes in bone marrow synthesize DNA.…”

Section: Materials and Methods A Preparationsupporting

confidence: 93%

Bone Marrow Lymphocytes: Their Isolation, Colony-Forming Capacity and Graft-Versus-Host Potential I. The Isolation and Characterization of Subpopulations of Bone Marrow Lymphocytes

Johnson

1971

Experimental Biology and Medicine

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Section: Materials and Methods A Preparationsupporting

confidence: 93%

Bone Marrow Lymphocytes: Their Isolation, Colony-Forming Capacity and Graft-Versus-Host Potential I. The Isolation and Characterization of Subpopulations of Bone Marrow Lymphocytes

Johnson

1971

Experimental Biology and Medicine

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“…At the same time, the slowly-renewing subpopulation actually increases in size. Previous studies in guinea-pigs and rats (Osmond & Everett, 1964;Harris & Kugler, 1965;Everett & Caffrey, 1967;Osmond, 1967;Brahim & Osmond, 1970) confirmed recently in mice (Kaiserman, M. Z., Miller, S . C. & Osmond, D. G., unpublished), demonstrate that the rapidly renewing small lymphocytes are locally produced by the division of precursor cells within the marrow.…”

Section: Discussionmentioning

confidence: 75%

Lymphocyte Populations in Mouse Bone Marrow: Quantitative Kinetic Studies in Young, Pubertal and Adult C3h Mice

Miller

Osmond

1975

Cell Proliferation

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Continuous 3H‐thymidine infusion was used to characterize two kinetic subpopula‐tions of small lymphocytes in mouse bone marrow during normal growth and development. Young (4 wk), pubertal (8 wk) and mature (16 wk) C3H mice were infused subcutaneously with 3H‐thymidine for periods up to 10 days. Femoral marrow was then examined in radioautographic smears. During the first 3 days the proportion of marrow small lymphocytes labelled by 3H‐thymidine showed a rapid exponential increase to 93%, 81% and 72% in 4 wk, 8 wk and 16 wk mice respectively. The rate of appearance of labelled small lymphocytes then declined markedly but remained higher in younger than in older animals. The labelling curves were found to represent the summation of two exponential curves from which the proportions and renewal rate of corresponding cell populations were calculated. Most marrow small lymphocytes comprised a rapidly renewing population but in mice of increasing age the relative incidence of these cells fell (93‐3% at 4 wk; 88‐0% at 8 wk; 78‐5% at 16 wk) and their half‐renewal time (T½) lengthened (14 hr at 4 wk; 18 hr at 8 wk; 24 hr at 16 wk). The remaining small lymphocytes were slowly renewing with mean T½ of 4, 7 and 14 days in 4, 8 and 16 wk mice, respectively. Some heavily labelled small lymphocytes persisted in the marrow up to 10 wk after fourteen daily 3H‐thymidine injections in 10–12 wk mice. The numbers of rapidly renewing cells decreased from 604 times 103 to 228 times 103 per mm3 of marrow from 4 wk to 16 wk, respectively, while slowly renewing cells increased from 44 times 103 to 61 times 103 per mm3. The total number of nucleated marrow cells per femur increased from 4 wk to 16 wk but the rapidly renewing small lymphocytes per femur fell in numbers by 36% and in renewal rate by 63%. The results demonstrate a selective change in bone marrow small lymphocytes with age; rapidly renewing cells decline in number and renewal rate while the number of slowly renewing cells increases. The concept of bone marrow as a primary lymphoid organ is discussed.

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“…A close relationship between the thymus and bone marrow lymphocytes has been demonstrated (14,31,37,38,56,80). There is strong evidence for the opinion that at least a portion and perhaps even the majority of bone marrow lymphocytes are formed in situ (39,40,62). Indeed, some investigations point in the direction that the pool of marrow lymphocytes is self-sustaining (14).…”

Section: Discussionmentioning

confidence: 96%

Transport of Tritium-Labelled DNA from the Thymus to Other Lymphoid Organs in Rabbits under Normal Conditions and after Administration of Endotoxin

Linna¹

1967

Int Arch Allergy Immunol

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An Investigation of Lymphocyte Production in Guinea-Pig Bone Marrow

Cited by 18 publications

References 11 publications

Bone Marrow Lymphocytes: Their Isolation, Colony-Forming Capacity and Graft-Versus-Host Potential I. The Isolation and Characterization of Subpopulations of Bone Marrow Lymphocytes

Bone Marrow Lymphocytes: Their Isolation, Colony-Forming Capacity and Graft-Versus-Host Potential I. The Isolation and Characterization of Subpopulations of Bone Marrow Lymphocytes

Lymphocyte Populations in Mouse Bone Marrow: Quantitative Kinetic Studies in Young, Pubertal and Adult C3h Mice

Transport of Tritium-Labelled DNA from the Thymus to Other Lymphoid Organs in Rabbits under Normal Conditions and after Administration of Endotoxin

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