An Investigation of Fluorescence Resonance Energy Transfer between Tryptophan and Quinine Sulphate

Abstract

Fluorescence resonance energy transfer (FRET) between tryptophan (Trp) and quinine sulphate (QS) has been investigated in aqueous medium at room temperature by absorption, emission, and time resolved fluorescence spectroscopy. Absorbance spectral characteristics rule out the possibility of ground state complex formation between them. Time resolved decay indicates dynamic type of quenching. Saturation of efficiency of energy transfer reveals the existence of FRET. Efficiency of energy transfer and distance between donor and acceptor pair were determined according to Förster's non radiative energy transfer theory.