An Investigation into the Potential Use of Chelating Agents and Antibiotics as Synchrony Inducers in the Fission Yeast Schizosaccharomyces pombe

Walker, Graeme M.; Duffus, John H.

doi:10.1099/00221287-114-2-391

Cited by 10 publications

(3 citation statements)

References 17 publications

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“…pombe (1-3 x lo6 cells ml-') were estimated by comparing the increase in cell numbers in treated cultures with that in untreated controls. Minimum inhibitory drug concentrations were used to synchronize cell division using the following modification of the pulse-induction method employed by Walker & Duffus (1979) for synchronization of S . pombe using chelating agents.…”

Section: E T H O D Smentioning

confidence: 99%

“…This contrasted with untreated control cells which possessed normal asynchronous division morphology in which cells were of variable length and around 10% of the population had cell plates. Lomofungin, which quickly and intensely stains S. pombe nuclei (Kopecka, 1976;Walker & Duffus, 1979), revealed that drug-treated cells failed to continue nuclear division after residual cell division had ceased. This was apparent from the discrete, spherical morphology of the nuclei which were located centrally in the cytoplasm.…”

Section: R E S U L T Smentioning

confidence: 99%

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Cell Cycle Specificity of Certain Antimicrotubular Drugs in Schizosaccharomyces pombe

Walker

1982

Microbiology

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Of the seven antimicrotubular drugs tested, nocodazole, mebendazole and trifluralin at saturable concentrations failed to inhibit cell division in Schizosaccharomyces pombe, while carbendazim, thiabendazole and chloropropham each at 50 micrograms ml- and amiprophos methyl at 200 micrograms ml-1 completely arrested cell division. This inhibition was associated with striking morphological changes in which carbendazim- and thiabendazole-treated cells became elongated and pseudohyphal, whereas chloropropham- and amiprophos methyl-treated cells appeared small and rounded with occasional V-shaped pairs. Lomofungin staining revealed that nuclear division was also arrested by these drugs. Suspected blockage of defined cell cycle stages was confirmed by pulse-induction experiments which revealed that cells could be synchronized into division using exposure to a drug for one generation. Further experiments with synchronous cultures prepared by size selection showed that different drugs possessed different transition points; for example, carbendazim and thiabendazole were effective in blocking a late stage of the cell cycle just prior to division, whereas amiprophos methyl affected a very early stage. The results suggest that some of the drugs used exert cell cycle specificity in S. pombe either by impairing microtubule assembly mechanisms (as with carbendazim and thiabendazole) or by inhibiting synthesis of tubulin subunits (as with amiprophos methyl). These drugs could prove useful in studies of microtubule biogenesis during the cell cycle in yeast.

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Section: E T H O D Smentioning

confidence: 99%

Section: R E S U L T Smentioning

confidence: 99%

Cell Cycle Specificity of Certain Antimicrotubular Drugs in Schizosaccharomyces pombe

Walker

1982

Microbiology

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“…0022-1287/80/0000-9165 $02.00 @ 1980 SGM Cellnumber determination. Cell number and cell plate index (the percentage of the total cell population in which a complete cell plate is visible) were determined as described previously by Walker & Duffus (1979).…”

Section: E T H O D Smentioning

confidence: 99%

Perturbation of Enzyme Activity in Cells of the Fission Yeast Schizosaccharomyces pombe Subjected to Continuous-flow Centrifugation

Walker¹,

Thompson²,

Slaughter³

et al. 1980

Microbiology

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Fluctuations in glutamine synthetase activity were observed when exponentially dividing cells of Schizosaccharomyces pombe were synchronized by continuous-flow centrifugation. These fluctuations were not seen in a control culture in which cells were allowed to grow asynchronously following filtration. However, when an asynchronous control culture was prepared by allowing cells to pass quickly through the continuous-action rotor without size selection, marked fluctuations in glutamine synthetase activity occurred. It is concluded that when the continuous-flow size selection method of cell synchronization is used, suitable control experiments are essential before any conclusions can be drawn about how enzyme synthesis is regulated during the cell cycle.

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Antifungal agents as tools in expermental mycology

Betina¹

1985

Folia Microbiol

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An Investigation into the Potential Use of Chelating Agents and Antibiotics as Synchrony Inducers in the Fission Yeast Schizosaccharomyces pombe

Cited by 10 publications

References 17 publications

Cell Cycle Specificity of Certain Antimicrotubular Drugs in Schizosaccharomyces pombe

Cell Cycle Specificity of Certain Antimicrotubular Drugs in Schizosaccharomyces pombe

Perturbation of Enzyme Activity in Cells of the Fission Yeast Schizosaccharomyces pombe Subjected to Continuous-flow Centrifugation

Antifungal agents as tools in expermental mycology

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