An inverted microcontact printing method on topographically structured polystyrene chips for arrayed micro-3-D culturing of single cells

Dusseiller, Marc R.; Schlaepfer, Dominik; Koch, Mirabai C.; Kroschewski, Ruth; Textor, Marcus

doi:10.1016/j.biomaterials.2005.02.032

Cited by 150 publications

(126 citation statements)

References 34 publications

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“…33) but having a Ϸ1,000-fold larger Young's modulus. The stiffness of the corresponding microposts was then typically Ϸ20,000 nN/ m. The posts were so stiff that the cells could not discriminate between the two directions.…”

Section: Resultsmentioning

confidence: 99%

Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates

Saez

Ghibaudo

Buguin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

349

306

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The physical properties of the cellular environment are involved in regulating the formation and maintenance of tissues. In particular, substrate rigidity appears to be a key factor dictating cell response on culture surfaces. Here we study the behavior of epithelial cells cultured on microfabricated substrates engineered to exhibit an anisotropic stiffness. The substrate consists of a dense array of micropillars of oval cross-section, so that one direction is made stiffer than the other. We demonstrate how such an anisotropic rigidity can induce directional epithelial growth and guide cell migration along the direction of greatest rigidity. Regions of high tractional stress and large cellular deformations within the sheets of cells are concentrated at the edges, in particular at the two poles of the islands along their long axis, in correlation with the orientation of actin stress fibers and focal adhesions. By inducing scattering activity of epithelial cells, we show that isolated cells also migrate along the direction of greatest stiffness. Taken together, these findings show that the mechanical interactions of cells with their microenvironment can be tuned to engineer particular tissue properties.cell migration ͉ cytoskeleton ͉ mecanotransduction ͉ microfabrication ͉ pattern formation

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Section: Resultsmentioning

confidence: 99%

Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates

Saez

Ghibaudo

Buguin

et al. 2007

Proc. Natl. Acad. Sci. U.S.A.

349

306

View full text Add to dashboard Cite

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“…Microwells can be obtained in PDMS, agarose or gelled collagen replicates of microfabricated templates (Dusseiller et al, 2005;Nelson and Chen, 2002;Nelson et al, 2006). Pulsed laser can be used to design holes in collagen gels (Liu et al, 2005b) or to photo-polymerise hydrogel for the purpose of guiding cells in 3D (Hahn et al, 2006;Lee et al, 2008;Liu Tsang et al, 2007).…”

mentioning

confidence: 99%

Micropatterning as a tool to decipher cell morphogenesis and functions

Théry

2010

Journal of Cell Science

644

560

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Cell architectureThe physiological cell microenvironment consists of extracellular matrix (ECM) fibres, adjacent cells and extracellular fluids, and the cell adhesion machinery is the first cellular component to encounter it. Upon binding of extracellular ligands, localised signalling is induced with subsequent assembly of the cytoskeleton. These localised events will affect the entire cell architecture, because the intracellular space is physically connected and SummaryIn situ, cells are highly sensitive to geometrical and mechanical constraints from their microenvironment. These parameters are, however, uncontrolled under classic culture conditions, which are thus highly artefactual. Micro-engineering techniques provide tools to modify the chemical properties of cell culture substrates at sub-cellular scales. These can be used to restrict the location and shape of the substrate regions, in which cells can attach, so-called micropatterns. Recent progress in micropatterning techniques has enabled the control of most of the crucial parameters of the cell microenvironment. Engineered micropatterns can provide a micrometer-scale, soft, 3-dimensional, complex and dynamic microenvironment for individual cells or for multi-cellular arrangements. Although artificial, micropatterned substrates allow the reconstitution of physiological in situ conditions for controlled in vitro cell culture and have been used to reveal fundamental cell morphogenetic processes as highlighted in this review. By manipulating micropattern shapes, cells were shown to precisely adapt their cytoskeleton architecture to the geometry of their microenvironment. Remodelling of actin and microtubule networks participates in the adaptation of the entire cell polarity with respect to external constraints. These modifications further impact cell migration, growth and differentiation. Journal of Cell Sciencemechanically supported by a dynamic equilibrium (Ingber, 2003; Ingber, 2006). Integrin-based cell adhesionIntegrins are transmembrane receptors that bind to ECM proteins and to intracellular actin filaments. When cells contact the ECM, they change their shape and spread in a multi-step process that includes cell attachment, formation of membrane protrusion, extension of cell membrane, and formation and contraction of stress fibers, which further stimulate cell attachment, membrane protrusion and cell shape extension.The attachment of the actin cytoskeleton to cell adhesions requires integrin clustering. Nanopatterning methods have led to determine the maximum distance of 60 nm between integrin molecules -a distance that still allows intracellular recruitment of actin filaments and signalling molecules (Arnold et al., 2004). Arrays of adhesive dots have been used to study the formation of filopodia and subsequent spreading steps. Depending on the cell type and the level of Rac activation, cells need a minimal distance between adhesion sites, so that filopodia can bridge them and promote cell spreading (Guillou et al., 2008;Lehnert et al., 2004). Th...

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“…One attractive aspect of micro/nano printing of polymers is that of introducing both topographical and surface chemistry changes into the material. This is evidenced by Ruiz et al [116] and Dusseiller et al [120] who showed that neural cell growth and epithelial cell growth can be manipulated with this technique, respectively. This technique has been advanced further by using proteins in the micro/nano contact printing [117][118][119] which allows for a more bio-functionalized surface to be developed.…”

Section: Micro/nano Contact Printingmentioning

confidence: 81%

“…This is on account of the fact that polymeric materials generally tend to be cheaper than metals and ceramics and can be seen in many instances to be easier to machine and manipulate to adapt the polymer for use in specific environments, especially biological environments. To this end, micro-and nano-printing has been developed and applied to polymeric materials [116][117][118][119][120][121]. A typical surface resulting from micro-contact printing can be seen in Figure 6.…”

Section: Micro/nano Contact Printingmentioning

confidence: 99%

Surface Treatments to Modulate Bioadhesion: A Critical Review

Waugh¹,

Toccaceli²,

Gillett³

et al. 2016

rev adhes adhesives

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On account of the recent increase in importance of biological and microbiological adhesion in industries such as healthcare and food manufacturing many researchers are now turning to the study of materials, wettability and adhesion to develop the technology within these industries further. This is highly significant as the stem cell industry alone, for example, is currently worth £3.5 million in the United Kingdom (UK) alone. This paper reviews the current state-of-the-art techniques used for surface treatment with regards to modulating biological adhesion including laser surface treatment, plasma treatment, micro/nano printing and lithography, specifically highlighting areas of interest for further consideration by the scientific community. What is more, this review discusses the advantages and disadvantages of the current techniques enabling the assessment of the most attractive means for modulating biological adhesion, taking in to account cost effectiveness, complexity of equipment and capabilities for processing and analysis.

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An inverted microcontact printing method on topographically structured polystyrene chips for arrayed micro-3-D culturing of single cells

Cited by 150 publications

References 34 publications

Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates

Rigidity-driven growth and migration of epithelial cells on microstructured anisotropic substrates

Micropatterning as a tool to decipher cell morphogenesis and functions

Surface Treatments to Modulate Bioadhesion: A Critical Review

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