An inverse-breathing encapsulation system for cell delivery

Wang, Long Hai; Ernst, Alexander U.; Flanders, James A.; Liu, Wanjun; Wang, Xi; Datta, Ashim K.; Epel, Boris; Kotecha, Mrignayani; Papas, Klearchos K.; Ma, Minglin

doi:10.1126/sciadv.abd5835

Cited by 44 publications

(47 citation statements)

References 61 publications

(94 reference statements)

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“…Inadequate oxygenation at subcutaneous space is another factor limit the viability of transplanted islets. Recently, Wang et al demonstrated that Inverse breathing Encapsulation Devise (iBED), a silicon-based gas exchangeable materials improves oxygen (O 2 ) delivery is encapsulated islets and shows prolonged survival in multiple xenograft models (88). Challenge of transplantation of these islets with macro-and micro-encapsulation methods at subcutaneous sites still remain present as more islets (>2-5 times) are required to achieve normal glycemia with devices compared to naked transplantation.…”

Section: Subcutaneous Space Transplantation Sitementioning

confidence: 99%

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

2021

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Diabetes is a complex disease that affects over 400 million people worldwide. The life-long insulin injections and continuous blood glucose monitoring required in type 1 diabetes (T1D) represent a tremendous clinical and economic burdens that urges the need for a medical solution. Pancreatic islet transplantation holds great promise in the treatment of T1D; however, the difficulty in regulating post-transplantation immune reactions to avoid both allogenic and autoimmune graft rejection represent a bottleneck in the field of islet transplantation. Cell replacement strategies have been performed in hepatic, intramuscular, omentum, and subcutaneous sites, and have been performed in both animal models and human patients. However more optimal transplantation sites and methods of improving islet graft survival are needed to successfully translate these studies to a clinical relevant therapy. In this review, we summarize the current progress in the field as well as methods and sites of islet transplantation, including stem cell-derived functional human islets. We also discuss the contribution of immune cells, vessel formation, extracellular matrix, and nutritional supply on islet graft survival. Developing new transplantation sites with emerging technologies to improve islet graft survival and simplify immune regulation will greatly benefit the future success of islet cell therapy in the treatment of diabetes.

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Section: Subcutaneous Space Transplantation Sitementioning

confidence: 99%

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

2021

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“…The reaction of CO 2 -lithium peroxide from an aqueous cellular environment by a gas-permeable membrane yielded a device that supported functional islets in retrieved mice for more than 2 months (L.H. Wang et al, 2021 ).…”

Section: Hydrogel-based Encapsulation Devicesmentioning

confidence: 99%

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Ghasemi

Akbari

Imani

2021

Front. Bioeng. Biotechnol.

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Islet transplantation provides a promising strategy in treating type 1 diabetes as an autoimmune disease, in which damaged β-cells are replaced with new islets in a minimally invasive procedure. Although islet transplantation avoids the complications associated with whole pancreas transplantations, its clinical applications maintain significant drawbacks, including long-term immunosuppression, a lack of compatible donors, and blood-mediated inflammatory responses. Biomaterial-assisted islet transplantation is an emerging technology that embeds desired cells into biomaterials, which are then directly transplanted into the patient, overcoming the aforementioned challenges. Among various biomaterials, hydrogels are the preferred biomaterial of choice in these transplants due to their ECM-like structure and tunable properties. This review aims to present a comprehensive overview of hydrogel-based biomaterials that are engineered for encapsulation of insulin-secreting cells, focusing on new hydrogel design and modification strategies to improve β-cell viability, decrease inflammatory responses, and enhance insulin secretion. We will discuss the current status of clinical studies using therapeutic bioengineering hydrogels in insulin release and prospective approaches.

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“…Encapsulation systems such as the TheraCyte macroencapsulation device system, enable protection of the grafts from host immune cell infiltration via a physical barrier (112)(113)(114)(115)(116). A gas exchangeable enhanced O2 supply device has been developed to aim for enhancing the graft survival (117)(118)(119). This macroencapsulation device system is being utilized in an ongoing clinical trial of stem cell-derived pancreatic progenitor cells or primary human islets in T1D patients (116,118).…”

Section: Immune Protection By Immunosuppressants and Macro/ Micro Encapsulation Devicementioning

confidence: 99%

Immune Protection of Stem Cell-Derived Islet Cell Therapy for Treating Diabetes

Tahbaz

Yoshihara

2021

Front. Endocrinol.

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Insulin injection is currently the main therapy for type 1 diabetes (T1D) or late stage of severe type 2 diabetes (T2D). Human pancreatic islet transplantation confers a significant improvement in glycemic control and prevents life-threatening severe hypoglycemia in T1D patients. However, the shortage of cadaveric human islets limits their therapeutic potential. In addition, chronic immunosuppression, which is required to avoid rejection of transplanted islets, is associated with severe complications, such as an increased risk of malignancies and infections. Thus, there is a significant need for novel approaches to the large-scale generation of functional human islets protected from autoimmune rejection in order to ensure durable graft acceptance without immunosuppression. An important step in addressing this need is to strengthen our understanding of transplant immune tolerance mechanisms for both graft rejection and autoimmune rejection. Engineering of functional human pancreatic islets that can avoid attacks from host immune cells would provide an alternative safe resource for transplantation therapy. Human pluripotent stem cells (hPSCs) offer a potentially limitless supply of cells because of their self-renewal ability and pluripotency. Therefore, studying immune tolerance induction in hPSC-derived human pancreatic islets will directly contribute toward the goal of generating a functional cure for insulin-dependent diabetes. In this review, we will discuss the current progress in the immune protection of stem cell-derived islet cell therapy for treating diabetes.

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An inverse-breathing encapsulation system for cell delivery

Cited by 44 publications

References 61 publications

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

Advances in Pancreatic Islet Transplantation Sites for the Treatment of Diabetes

An Overview of Engineered Hydrogel-Based Biomaterials for Improved β-Cell Survival and Insulin Secretion

Immune Protection of Stem Cell-Derived Islet Cell Therapy for Treating Diabetes

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