“…Consequently, to facilitate biological treatment, this type of wastewater is supplemented with chemical fertilizer (nitrogen and phosphorus) in order to achieve the recommended C/N/P ratio of 100:5:1 (COD/N/P of 100:3.5:0.8), required for an efficient biological treatment of the organic load (Dennis et al, 2004;Gray, 2004;Pratt et al, 2007;. But nutrient supplementation is expensive, difficult to control, requires extensive monitoring, and sometimes causes an excess of nutrient in the effluent; alternatively, the nitrogen necessary for bacterial growth could be obtained through BNF or nutrient recycling from benthal deposits (Hubbe et al, 2016;Wiegand et al, 2014). Some studies have determined the conditions required to convert and/or operate wastewater treatment facilities under nitrogen fixation mode or nutrient recycling from benthal deposits, adding limited or no nutrients to the system (Pratt et al, 2007;Wiegand et al, 2014).…”