An Introduction to Forensic DNA Analysis, Second Edition

Rudin, Norah; Inman, Keith

doi:10.1201/9781420058505

Cited by 39 publications

(41 citation statements)

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“…To obtain the allelic frequency, one looks up the population frequency for each band in the Promega-supplied allelic frequency table (Technical Manual Number D004). Following calculation of probability at each locus, the product rule was applied to calculate the probability of a match across all three loci [9,11]. A detailed example calculation for individual number 1 from Fig.…”

Section: Table I Allelic Numbering For Each Individual's Banding Pattmentioning

confidence: 99%

DNA fingerprint analysis of three short tandem repeat (STR) loci for biochemistry and forensic science laboratory courses

McNamara-Schroeder

Olonan

Chu

et al. 2006

Biochem Molecular Bio Educ

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We have devised and implemented a DNA fingerprinting module for an upper division undergraduate laboratory based on the amplification and analysis of three of the 13 short tandem repeat loci that are required by the Federal Bureau of Investigation Combined DNA Index System (FBI CODIS) data base. Students first collect human epithelial (cheek) cells using sterile buccal swabs and then utilize commercially available kits and materials to extract genomic DNA. This is followed by the PCR amplification of three specific short tandem repeat loci (i.e. CSF1PO, TPOX, THO1). Polyacrylamide gel electrophoresis is used to resolve the allelic bands associated with the three short tandem repeat loci, and the results are statistically analyzed in the context of human population genetics. In addition, DNA was collected from a family, and the children's allele sets were compared with those of the parents to help illustrate paternal and maternal relatedness. This module enables students to use the materials and methods employed by actual law enforcement agencies and therefore can be used for laboratory exercises in traditional biochemistry curricula as well as for the growing field of forensic science and education.

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Section: Table I Allelic Numbering For Each Individual's Banding Pattmentioning

confidence: 99%

DNA fingerprint analysis of three short tandem repeat (STR) loci for biochemistry and forensic science laboratory courses

McNamara-Schroeder

Olonan

Chu

et al. 2006

Biochem Molecular Bio Educ

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“…Contamination is defined as the inadvertent addition of an individual's DNA during or after collection of the evidence sample [2] and may thus occur both at the crime scene and in the laboratory. Especially low template (LT) DNA analysis, i.e.…”

Section: Introductionmentioning

confidence: 99%

Sources of DNA Contamination and Decontamination Procedures in the Forensic Laboratory

Vandewoestyne¹

2011

J Forensic Res

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ForensicResearch Vandewoestyne et al. J Forensic Res 2011, S2 http://dx.doi. AbstractThe sensitivity of forensic DNA typing techniques can cause problems when evidence samples are inadvertently contaminated with DNA from another source. Therefore, precautions need to be taken to minimize the risk of contamination. In this study, laboratory air and surfaces, tools and equipment were evaluated as potential sources of contaminating DNA. Subsequently, two decontamination procedures, i.e. the conventionally used sodium hypochlorite and the commercially available DNA decontamination solution DNA ZAP TM (Applied Biosystems), were compared for their use in removing potentially contaminating DNA from the laboratory working environment.From our results, it can be concluded that air is unlikely to be the source of observed DNA contamination in the laboratory whereas DNA accumulating on surfaces, tools and equipment within the laboratory environment may potentially be transferred to evidence samples. DNA ZAP TM outperformed the conventionally used sodium hypochlorite decontamination procedure. Stringent preventive measures and decontamination of equipment and laboratory surfaces is important to avoid secondary transfer of this contaminating DNA to evidence samples.

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“…Since sample collection is typically done at the crime scene and is out of the control of the lab, we will not focus on this step. Clean-up and isolation is typically done using one of several methods: organic extraction, column extraction and Chelex extraction [6][7][8][9]. Once cleaned and isolated, analysis is typically performed by PCR.…”

Section: Introductionmentioning

confidence: 99%

“…PCR is an enzyme-driven process for exponentially amplifying specific DNA sequences of interest by repetitive temperature cycling. Forensics laboratories typically use PCR to look for unique identification signatures present in DNA sequences [7,9]. Recently, investigators have begun to look at sequences of mitochondrial DNA, which are more abundant in a given sample [11,12].…”

Section: Introductionmentioning

confidence: 99%

Comparison of Packed Beds and Qiagen Columns for Recovering Trace Amounts of B. anthracis DNA from Liquid Suspensions

Sorensen¹,

Arroyo²,

Erler³

et al. 2006

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Executive SummaryThe goal of this work was to optimize and evaluate LLNL's in-bed amplification technology to improve the level of detection for suspensions containing trace amounts of anthracis DNA. The binding/cleaning performance of the packed bed is compared to the conventional commercial approach; Qiagen column cleanup and elution, followed by detection through an ex-situ amplification process.Five liquid suspensions were spiked with B.anthracis DNA in concentration series. These suspensions were: 1) water, 2) water with EDTA, 3) dirty water from carpet extraction, 4) dirty carpet extraction with phosphate buffered saline (PBS) plus 0.1% Tween 20 plus 0.1% gelatin, and 5) a subway aerosol collected in water. Each suspension matrix was spiked with DNA and injected (in replicate) into either Qiagen Microcolumns (using the kit processing instructions) or LLNL's packed bed (using the LLNL in-bed purification and amplification protocol). The process output was assayed by quantitative polymerase chain reaction (QPCR). Table ES-1 shows the level of DNA (pg per 100 uL of input suspension) that resulted in successful amplification for all reactions (X=Y), and the level for which at least one of the reactions was successful (X>0). For each suspension and DNA concentration, there were Y QPCR assays of which X showed successful amplification.LLNL's packed bed technology outperformed Qiagen Microcolumns for all five suspensions, typically by one order of magnitude in both the limit of assured detection (all reactions positive), and the lower limit of detection (some reactions positive). Limit of assured detection (all PCR rxns successful)Lower limit of detection (X successful / Y rxns)

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An Introduction to Forensic DNA Analysis, Second Edition

Cited by 39 publications

References 0 publications

DNA fingerprint analysis of three short tandem repeat (STR) loci for biochemistry and forensic science laboratory courses

DNA fingerprint analysis of three short tandem repeat (STR) loci for biochemistry and forensic science laboratory courses

Sources of DNA Contamination and Decontamination Procedures in the Forensic Laboratory

Comparison of Packed Beds and Qiagen Columns for Recovering Trace Amounts of B. anthracis DNA from Liquid Suspensions

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