An Intravenous Bolus of Epa

Burban, Mélanie; Meyer, Grégory; Olland, Anne; Séverac, François; Yver, Blandine; Toti, Florence; Schini‐Kerth, Valérie B.; Meziani, Ferhat; Boisramé-Helms, Julie

doi:10.1097/shk.0000000000000624

Cited by 11 publications

(4 citation statements)

References 35 publications

(29 reference statements)

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“…Supplementation with the DHA diet diminished the superoxide anion levels near to control levels, similar to those reported in the aorta of these animals [24]. In line with these results, is the ability of n-3 PUFAs to decrease superoxide anion production in mouse aorta [38] and human fibroblasts [39]. In addition, DHA has been reported to decrease oxidative stress in vascular [40], nervous [41] and immune [42] systems.…”

Section: Discussionsupporting

confidence: 75%

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

et al. 2017

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Over the past few decades, the cardiovascular benefits of a high dietary intake of long-chain polyunsaturated fatty acids (PUFAs), like docosahexaenoic acid (DHA), have been extensively studied. However, many of the molecular mechanisms and effects exerted by PUFAs have yet to be well explained. The lack of sex hormones alters vascular tone, and we have described that a DHA-supplemented diet to orchidectomized rats improve vascular function of the aorta. Based on these data and since the mesenteric artery importantly controls the systemic vascular resistance, the objective of this study was to analyze the effect of a DHA-supplemented diet on the mesenteric vascular function from orchidectomized rats. For this purpose mesenteric artery segments obtained from control, orchidectomized or orchidectomized plus DHA-supplemented diet were utilized to analyze: (1) the release of prostanoids, (2) formation of NO and ROS, (3) the vasodilator response to acetylcholine (ACh), as well as the involvement of prostanoids and NO in this response, and (4) the vasoconstrictor response to electrical field stimulation (EFS), analyzing also the effect of exogenous noradrenaline (NA), and the NO donor, sodium nitroprusside (SNP). The results demonstrate beneficial effects of DHA on the vascular function in orchidectomized rats, which include a decrease in the prostanoids release and superoxide formation that were previously augmented by orchidectomy. Additionally, there was an increase in endothelial NO formation and the response to ACh, in which NO involvement and the participation of vasodilator prostanoids were increased. DHA also reversed the decrease in EFS-induced response caused by orchidectomy. All of these findings suggest beneficial effects of DHA on vascular function by reversing the neurogenic response and the endothelial dysfunction caused by orchidectomy.

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Section: Discussionsupporting

confidence: 75%

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

et al. 2017

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“…These data suggest that DHA pretreatment has physiological consequences on the development of MI-induced LV dysfunction, preserving cardiac function in mice. The observed DHA-induced attenuation of MI-induced LV dysfunction adds to cardioprotective effects of other omega-3 FA in a Langendorff perfusion model reporting that an intravenous bolus of EPA: DHA 6: 1 protects against myocardial ischemia-reperfusion-induced injury [ 23 ]. Furthermore, preserved LV function in DHA-pretreated mice 14 days after MI is in line with the clinical data from the OMEGA-REMODEL trial, demonstrating that in humans, the combination of DHA and EPA ω -3 FA ethyl ester supplementation beginning after the onset of STEMI improved LV function after 6 months.…”

Section: Discussionmentioning

confidence: 99%

“…Consequently, supplementation is recommended for patients with prevalent CHD such as a recent MI [ 21 , 22 ]. DHA is either obtained from diet but can also be synthesized from EPA [ 23 , 24 ]. Thus, various investigations used combinations of DHA and EPA to investigate ω -3 FA effects on cardiac health.…”

Section: Introductionmentioning

confidence: 99%

DHA Supplementation Attenuates MI-Induced LV Matrix Remodeling and Dysfunction in Mice

Habicht

Mohsen

Eichhorn

et al. 2020

Oxidative Medicine and Cellular Longevity

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Objective. Myocardial ischemia and reperfusion (I/R) injury is associated with oxidative stress and inflammation, leading to scar development and malfunction. The marine omega-3 fatty acids (ω-3 FA), eicosapentaenoic acid (EPA), and docosahexaenoic acid (DHA) are mediating cardioprotection and improving clinical outcomes in patients with heart disease. Therefore, we tested the hypothesis that docosahexaenoic acid (DHA) supplementation prior to LAD occlusion-induced myocardial injury (MI) confers cardioprotection in mice. Methods. C57BL/6N mice were placed on DHA or control diets (CD) beginning 7 d prior to 60 min LAD occlusion-induced MI or sham surgery. The expression of inflammatory mediators was measured via RT-qPCR. Besides FACS analysis for macrophage quantification and subtype evaluation, macrophage accumulation as well as collagen deposition was quantified in histological sections. Cardiac function was assessed using a pressure-volume catheter for up to 14 d. Results. DHA supplementation significantly attenuated the induction of peroxisome proliferator-activated receptor-α (PPAR-α) (2.3±0.4 CD vs. 1.4±0.3 DHA) after LAD occlusion. Furthermore, TNF-α (4.0±0.6 CD vs. 1.5±0.2 DHA), IL-1β (60.7±7.0 CD vs. 11.6±1.9 DHA), and IL-10 (223.8±62.1 CD vs. 135.5±38.5 DHA) mRNA expression increase was diminished in DHA-supplemented mice after 72 h reperfusion. These changes were accompanied by a less prominent switch in α/β myosin heavy chain isoforms. Chemokine mRNA expression was stronger initiated (CCL2 6 h: 32.8±11.5 CD vs. 78.8±13.6 DHA) but terminated earlier (CCL2 72 h: 39.5±7.8 CD vs. 8.2±1.9 DHA; CCL3 72 h: 794.3±270.9 CD vs. 258.2±57.8 DHA) in DHA supplementation compared to CD mice after LAD occlusion. Correspondingly, DHA supplementation was associated with a stronger increase of predominantly alternatively activated Ly6C-positive macrophage phenotype, being associated with less collagen deposition and better LV function (EF 14 d: 17.6±2.6 CD vs. 31.4±1.5 DHA). Conclusion. Our data indicate that DHA supplementation mediates cardioprotection from MI via modulation of the inflammatory response with timely and attenuated remodeling. DHA seems to attenuate MI-induced cardiomyocyte injury partly by transient PPAR-α downregulation, diminishing the need for antioxidant mechanisms including mitochondrial function, or α- to β-MHC isoform switch.

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“…In the last few years, many animal studies have shown that n-3 PUFAs might have good effects on MI/R injury. In a rat model of acute MI/R, intravenous injection of n-3 PUFAs before reperfusion reduced vascular failure and shock caused by I/R (35). A diet rich in n-3 PUFA from fish oil affected heart function and improved cardiac responses to I/R via reducing oxygen consumption and increasing post-ischemic recovery (36).…”

Section: Discussionmentioning

confidence: 99%

Identification of potential molecular mechanisms and small molecule drugs in myocardial ischemia/reperfusion injury

Jiang

Liu

Chen

et al. 2020

Braz J Med Biol Res

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Myocardial ischemia/reperfusion (MI/R) injury is a complex phenomenon that causes severe damage to the myocardium. However, the potential molecular mechanisms of MI/R injury have not been fully clarified. We identified potential molecular mechanisms and therapeutic targets in MI/R injury through analysis of Gene Expression Omnibus (GEO) database. Differentially expressed genes (DEGs) were found between MI/R injury and normal samples, and overlapping DEGs were found between GSE61592 and GSE67308. Gene Ontology (GO) and pathway analysis were performed for overlapping DEGs by Database for Annotation, Visualization and Integration Discovery (DAVID). Then, a network of protein-protein interaction (PPI) was constructed through the Search Tool for the Retrieval of Interacting Genes (STRING) database. Potential microRNAs (miRNAs) and therapeutic small molecules were screened out using microRNA.org database and the Comparative Toxicogenomics database (CTD), respectively. Finally, we identified 21 overlapping DEGs related to MI/R injury. These DEGs were significantly enriched in IL-17 signaling pathway, cytosolic DNA-sensing pathway, chemokine signaling, and cytokine-cytokine receptor interaction pathway. According to the degree in the PPI network, CCL2, LCN2, HP, CCL7, HMOX1, CCL4, and S100A8 were found to be hub genes. Furthermore, we identified potential miRNAs (miR-24-3p, miR-26b-5p, miR-2861, miR-217, miR-4251, and miR-124-3p) and therapeutic small molecules like ozone, troglitazone, rosiglitazone, and n-3 polyunsaturated fatty acids for MI/R injury. These results identified hub genes and potential small molecule drugs, which could contribute to the understanding of molecular mechanisms and treatment for MI/R injury.

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An Intravenous Bolus of Epa

Abstract: In this rat model of myocardial IR, an intravenous Omega-3 bolus before reperfusion decreases IR-induced vascular failure and shock. These results open therapeutic perspectives as far as myocardial reperfusion process is concerned that deserve further explorations in humans.

Cited by 11 publications

References 35 publications

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

Docosahexaenoic Acid Supplemented Diet Influences the Orchidectomy-Induced Vascular Dysfunction in Rat Mesenteric Arteries

DHA Supplementation Attenuates MI-Induced LV Matrix Remodeling and Dysfunction in Mice

Identification of potential molecular mechanisms and small molecule drugs in myocardial ischemia/reperfusion injury

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