An interplay between cellular growth and atypical fusion defines morphogenesis of a modular glial niche in Drosophila

Rujano, Maria A.; Delic, Bojana; Marc, Julie; Spéder, Pauline

doi:10.1038/s41467-022-32685-3

Cited by 13 publications

(24 citation statements)

References 100 publications

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“…1D). CG network is progressively built around NSC during larval development in a process which parallels NSC behaviour [21,24,25] (Fig. 1E).…”

Section: Introductionmentioning

confidence: 78%

“…For the Nrx-IV antibody, CNS were fixed for 3 minutes in Bouin’s fixative solution (Sigma Aldrich, HT10132), and the rest of the protocol was identical. Primary antibodies used were: guinea pig anti-Dpn (1:5000,[25]), chicken anti-GFP (1:2000, Abcam ab13970), rat anti-ELAV (1:100, 7E8A10-c, DSHB), mouse anti-ELAV (1:100, 9F8A9-c, DSHB), rat anti-dE-cadherin (1:50, DCAD2, DSHB), mouse anti-Armadillo (1:50, N2 7A1, DSHB), rabbit anti-Repo (1:10000, kind gift from B. Altenheim), mouse anti-Repo 1:100 (DSHB, 8D12-c), mouse anti-Prospero (1:100, MR1A, DSHB), rabbit anti-Asense (1:3000, kind gift from the Yan lab). rabbit anti-Phospho-histone H3 (1:100, Millipore 06-570), rabbit anti-Nrx-IV (1:1000, [46]) mouse anti-wrapper (1:20, DSHB 10D3, supernatant), mouse anti-Nrg 180 (1:50, DSHB BP104, supernatant).…”

Section: Methodsmentioning

confidence: 99%

“…For the Nrx-IV antibody, CNS were fixed for 3 minutes in Bouin's fixative solution (Sigma Aldrich, HT10132), and the rest of the protocol was identical. Primary antibodies used were: guinea pig anti-Dpn (1:5000, [25]), chicken anti-GFP (1:2000, Abcam ab13970), rat anti-ELAV…”

Section: Fixed Tissue Immunohistochemistry and Imagingmentioning

confidence: 99%

“…1D). CG network is progressively built around NSC during larval development in a process which parallels NSC behaviour [21,24,25] (Fig.1E). CG cells, born during embryogenesis, do not form a continuous meshwork nor encase quiescent NSCs at larval hatching.…”

Section: Introductionmentioning

confidence: 99%

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A corset of adhesions during development establishes individual neural stem cell niches and controls adult behaviour

Banach-Latapy

Rincheval

Guénal

et al. 2022

Preprint

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Neural stem cells (NSCs) reside in a defined cellular microenvironment, the niche, which supports the generation and integration of neuronal lineages. The mechanisms building a sophisticated niche structure around NSCs, and their functional relevance for neurogenesis are yet to be understood. In the Drosophila larval brain, the cortex glia (CG) encase individual NSC lineages, organizing the stem cell population and newborn neurons into a stereotypic structure. We first found that lineage information is dominant over stem cell fate. We then discovered that, in addition to timing, the balance between multiple adhesion complexes supports the individual encasing of NSC lineages. An intra-lineage adhesion through homophilic Neuroglian interactions provides strong binding between cells of a same lineage, while a weaker interaction through Neurexin-IV exists between CG to NSC lineages. Their loss leads to random, aberrant grouping of several NSC lineages together, and to altered axonal projection of newborn neurons. Further, we link the loss of these two adhesion complexes during development to locomotor hyperactivity in the resulting adults. Altogether, our findings identify a corset of adhesions building a neurogenic niche at the scale of individual stem cell and provide the proof-of-principle that mechanisms supporting niche formation during development define adult behaviour.

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“…1D). CG network is progressively built around NSC during larval development in a process which parallels NSC behaviour [21,24,25] (Fig. 1E).…”

Section: Introductionmentioning

confidence: 78%

Section: Methodsmentioning

confidence: 99%

Section: Fixed Tissue Immunohistochemistry and Imagingmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

A corset of adhesions during development establishes individual neural stem cell niches and controls adult behaviour

Banach-Latapy

Rincheval

Guénal

et al. 2022

Preprint

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“…3). Recently, it has been shown that cortical glia increases their nuclei number and extend membrane network through endomitosis (Rujano et al, 2022).…”

Section: Cut Defective Cortical Glial Cells Are Unable To Increase Th...mentioning

confidence: 99%

Cut homeodomain transcription factor is a novel regulator of cortex glia morphogenesis and maintenance of neural niche

Yadav¹,

Das²,

Mishra³

et al. 2022

Preprint

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Cortical glia in Drosophila central nervous system forms a niche around neural stem cells (NSCs) and their progeny for necessary signals to establish cross-talk with their surroundings. These cells grow and expand their thin cytoplasmic extensions around neural cell bodies in the nervous system. Although essential for the development and function of the nervous system, how these cells make extensive and intricate membrane networks remain largely unknown. Here we show that Cut, a homeodomain transcription factor, directly regulates the fate of the cortical glial cells impacting NSC homeostasis. Focusing on the thoracic ventral nerve cord (tVNC), we found that Cut is required for normal growth and development of cortical glial cells. We also highlight that levels of Cut expression play an essential role in the cytoplasmic membrane network growth around the neural cells. Loss of Cut in cortical glia results in a substantial reduction in their cytoplasmic extensions and network around cell bodies of NSCs and their progeny, whereas its overexpression induces the overall growth of cortical glia main branches at the expense of side ones. We also note a striking gain in the nuclear size and volume of cortical glial cells upon Cut overexpression. Furthermore, constitutively high Cut levels increase DNA content in these cells more than threefold, indicating an interference with the splitting of nuclei during endomitosis. Since cortical glia makes syncytial membrane networks around neural cells, the finding identifies Cut as a regulator of glial growth and endomitosis to support a functional nervous system. It is the first report that highlights a novel function of Cut in regulating the growth and branching of cortical glial cells and control over endomitosis.

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Drosophila glia take shape to sculpt the nervous system

Corty

Coutinho-Budd

2023

Current Opinion in Neurobiology

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An interplay between cellular growth and atypical fusion defines morphogenesis of a modular glial niche in Drosophila

Cited by 13 publications

References 100 publications

A corset of adhesions during development establishes individual neural stem cell niches and controls adult behaviour

A corset of adhesions during development establishes individual neural stem cell niches and controls adult behaviour

Cut homeodomain transcription factor is a novel regulator of cortex glia morphogenesis and maintenance of neural niche

Drosophila glia take shape to sculpt the nervous system

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